production of highly purified bovine transferrin group 5b arjun goel, connie marcelli, lianne kark,...

PRODUCTION OF HIGHLY PURIFIED BOVINE

TRANSFERRIN

Group 5BArjun Goel, Connie Marcelli,

Lianne Kark, Sara Dang, Vivien Tai

Faculty Advisor Prof C.A. Mims

TimetableTask

3 10 17 24 1 8 15 22 29 5 12 19 26Obtain Chemical & Physical Info and Thermodynamic & Kinetic DataAlternatives Available within design briefFirst ReportScalingProject Background

24-Sep

Second Report--Scope of Design; Timetable of Design; Allocation of Jobs within Team

9-OctProcess Flow Diagram NO MASS & ENERGY BALANCES

22-Oct Basis of Design Note

26-Oct

Frozen PFD w/ Mass & Energy Balances; Major Process Streams; Major Process EquipmentSpecification of Process Equipment--Sizing Based on Mass Balance; Auxiliary Equipment Specification; Material Selection; Waste recycle/treatment/disposalKEY UNIT DESIGNP&ID SafetyEnvironmental ImpactControlControl & Operating Philosophy--Normal OperationControl & Operating Philosophy--Startup/Shutdown OperationPlant Layout--General; Safety & Fire; Spacing RecommendationsCost Estimates--Capital Costs; Operating Costs; Selling Price/Unit Price; IRR/NPV analysisEconomic FeasibilityEnergy Efficiency

1-Nov Scheme of Final Report9-Nov Present Preliminary Layout

26-Nov Final Report & DrawingsProcess UtilitiesRecommendationsSocio Economic ImpactEditorExecutive Summary

September October November

Agenda

Mass Balances & Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Process Overview

Crude Paste

Filtration

Chromatography

Dry Transferrin Powder

Agenda

Mass Balances & Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Mass Balance — FeedsStarting Paste

18 kg

D.I. Water 571 L

Celite 6 kg

FeCl3, NaHCO3, Na-Citrate for iron Binding

Diafiltration BuffersWash for

Column

EDTA for Iron Chelating

Process

Distilled Water 160 L

Mass Balance — Products

Water 740 L

Celite 6 kg

Inorganics FeCl3, NaHCO3,

Na-Citrate, EDTA, NaOH

Purified Apotransferrin 350g / Batch

Process

Trace Proteins

Ethanol 4.5 kg

Solids Liquids

Immunoglobins for recovery

Waste Treatment--LiquidsOption 1 Option 2

NeutralizeAcid/Base Addition

To Sewer

Waste Water

Solid Waste

Waste Water

Process

Reverse Osmosis

Ion Exchange

Waste Treatment — Solids

1. Reverse Osmosis cartridges

2. Filter press cakes

Disposal via contractor

• Mainly salts and celite trapped in filters

Agenda

Mass Balances & Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Key Unit: R-101

Wet Paste

2

4

5

R-101

Celite Filter Aid

3

Deionized water

NaOH

P-101

1

Key Unit: R-101

• Stirred Tank• Cylindrical• Conical Bottom • Tank Material: SS316• In Cold Room 0-5 oC

Key Unit: R-101

• Volume of solution in tank: 400 L

• Unit Selected:– Capacity: 757 L ( 200 Gal )– Depth: 1.46 m– Diameter: 0.91m

Key Unit Accessories

• Level Indicator

• pH Monitor

• Temperature Indicator

• SS316 piping

• Valve

• Air Diaphragm Pump

• Mixer

Agenda

Mass Balances &Waste Treatment

Key Unit Discussion

Unit Specifications

Current Challenges

Filter Press: FP-101

• 12” in diameter• Stainless Steel

plates and frames• Cellulose Pads• Four 1” sludge

frames• 40 psi• Cost: $6 703

Filter Press: FP-1013 Applications:

1) 4 Plates: removes 12 000 g paste @ 19 L/min

2) 2 Plates: removes 1 320 g denatured protein @ 9 L/min

3) 2 Plates: collects 3 600 g precipitated protein @ 9 L/min

PrefiltrationSF-101

• Before ultrafiltration

• Millipore low protein binding cartridge filter

• Filtration Area: 0.7m2

• 80 psi

• Entrains particles > 0.5 m

• US$208 for 2

Ultrafiltration

Spiral Wound FilterUF-101

• Concentration: 450 L to 90 L

• Polysulfone

• Flow: 100 L/hr

• Flux: 22 L/m2 hr

• Pressure: 60 PSI

• Cost: $8 212

Spiral Wound FilterUF-102

• Concentrate from 30L to 15L

• Regenerated cellulose Membrane

• Pressure: 60 PSI

• Flow: 10L/hr

• Flux: 11 L/m2hr

• Cost: $2 154

Hollow FiberUF-201

• Concentrate from 12 L to 6 L

• Polysulfone• 25 PSI• 6 L/hr• 30 L/ m2hr• Peristaltic Pump

DEAE Column

1

C-201

To Waste

To Waste5

DEAE Sepharose Fast Flow Column: C-201

• Automated

• Includes: The Column

The Bioprocess Skid

The computer

DEAE Resin

• Total Cost: CND$211, 000

Water Distillation ColumnT-204

• Rate: 190 L/24 hrs• Storage capacity:

280 L• Size:

2m x 1m x 0.6m

• Cost : US$ 3 945

Deionized Water UnitI-101

• Flow range:

15 – 23 L/min• Size:

1.5m x 0.6m x 0.4m• Cost:

US$ 4 142

LyophilizerL-101

• Similar unit used in

plant

• Capacity: 8-12 L• ~6 L of water to

condense per batch

Future Directions

• Key unit development– P&ID– Design equations– Control Philosophy

• Economic Evaluation

• Plant Layout

• Appendices