mesenchymal stem cell concentration and bone repair: potential pitfalls from bench to bedside by...

Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside

by Anna V. Cuomo, Mandeep Virk, Frank Petrigliano, Elise F. Morgan, and Jay R. Lieberman

J Bone Joint Surg AmVolume 91(5):1073-1083

May 1, 2009

©2009 by The Journal of Bone and Joint Surgery, Inc.

CFU-Fs were counted to estimate the mesenchymal stem cell concentrations.

Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083

©2009 by The Journal of Bone and Joint Surgery, Inc.

The nucleated cell count of the bone marrow aspirate (BMA) was closely correlated to the mesenchymal stem cell (MSC) concentration of the bone marrow aspirate.

Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083

©2009 by The Journal of Bone and Joint Surgery, Inc.

Radiographs of femoral defects in Groups I through VI at twelve weeks.

Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083

©2009 by The Journal of Bone and Joint Surgery, Inc.

Radiographic scores were determined by the percentage of bridging bone at the defect site (1 = 0% to 25%, 2 = 26% to 50%, 3 = 51% to 75%, 4 = 76% to 99% or unicortical bridging of the defect,

and 5 = 100% or bicortical bridging of the defect).

Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083

©2009 by The Journal of Bone and Joint Surgery, Inc.

Micro-computed tomography scans showing the bone formation within the femoral defects in Groups III, IV, and V at twelve weeks.

Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083

©2009 by The Journal of Bone and Joint Surgery, Inc.

Histologic cross sections at the midpoint of the femoral defect.

Anna V. Cuomo et al. J Bone Joint Surg Am 2009;91:1073-1083

©2009 by The Journal of Bone and Joint Surgery, Inc.