measuring retinal function with erg in rodents

Measuring Retinal Function with ERG in Rodents

Sponsored by:

Christine van Hover, PhD.

Field Application Specialist,Phoenix Research Labs

Peter Lundh von Leithner, PhD.

Research Scientist,University College London

InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in

the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools

and laboratory services.

Thank you to our event sponsor

Copyright 2016 Phoenix Research Labs and InsideScientific. All Rights Reserved.

Christine van Hover, PhD.Field Application Specialist,Phoenix Research Labs

Sponsored by:

Measuring Retinal Function with ERG in Rodents

Phoenix Research Labs Electroretinography

What is ERG?

Measuring the function of retinal cells by detecting their electrical activity in response to flashes of light

Phoenix has developed technology to enable a four step process

1. Prepare animal

2. Establish corneal contact

3. Acquire data

4. Analyze and present data

Ganzfeld ERG Focal ERG

• Dark lab technology enables easy set up and dark adaptation

• Needle electrodes are easy to place and secure

• ERG systems specifically designed for rodent eyes

1. Prepare Animal

• Near infrared light allows image-guided alignment with solid contact electrode

• Unique Maxwellian view allows uniform illumination of the eye

• Live chart mode shows real time coupling with the cornea

2. Establish corneal contact

2. Establish corneal contact

• Near infrared light allows image-guided alignment with solid contact electrode

• Unique Maxwellian view allows uniform illumination of the eye

• Live chart mode shows real time coupling with the cornea

3. Acquire Data

Before

After

• Simple interface to select protocol and collect data

• Clean algorithm removes 50 Hz or 60 Hz noise

• Allows detection of small differences

• Fewer light flashes

• No Faraday cage needed

• LabScribe calculates A and B waves

• Export graph and table of results

• Oscillatory potentials can be examined separately

• Automatic waterfall provides effective review

4. Analyze and present data

4. Analyze and present data

• LabScribe calculates A and B waves

• Export graph and table of results

• Oscillatory potentials can be examined separately

• Automatic waterfall provides effective review

Copyright 2016 P. Lundh von Leithner, Phoenix Research Labs and InsideScientific. All Rights Reserved.

Peter Lundh von Leithner, PhDResearch Scientist,University College London

Measuring Retinal Function with ERG in Rodents

Measuring Retinal Function using

focal ERG in Rodents

1. Who we are and what we do

2. How we generate Electroretinography (ERG) data

3. How we evaluate ERG data sets in the context ofpharmacological assays

4. Study examples

Institute of Ophthalmology UCLgoo.gl/q5zEbw

Moorfields Eye Hospitaltinyurl.com/hmz7um9

University College Londongoo.gl/gy5f

Translational Vision Research (TVR) Lab

• Professor Dave Shima

• Identify novel functions of vascular

growth factors (VGF and their

potential efficacy as therapeutic

targets

• VEGF role in neuron migration in

development and as a

neuroprotectant in the adult retina

• Present efforts are focused on

determine the neurorotectiove roles of

VGF in models of DR and Glaucoma

TVR In Vivo

• Fluorophotometry

• Perfusion assays

• Electroretinography

• Fluorescence funduscopy

• Scanning Laser Ophthalmoscopy

• Optical Coherence Tomography

Electroretinography (ERG) is a physiological assay

used to monitor retinal function of the retina. Specifically,

the function of the light-sensitive cells of the eye, the rods

and cones, and their connecting ganglion cells in the

retina are examined.

Preclinical electroretinography

• Developing physiological assays to assess the degree of retinal

function in models of vision

• Phenotyping of rodent models with including visual signal processing

• Monitoring sensory performance during and after therapeutic

interventions.

Amplification of signal

Animal Handling

Ketamine+Xylazine

Hypnovel+Domitor+Sublimaze

Isoflurane

Tropicamide 1%

Phenelypherine 1%

Photopic

Photopic

25

Stimulator settings

Scotopic

27

Scotopic

Micron Discover settings

28

31

33

fERGFFA

References

• Doyle, S. L., López, F. J., Celkova, L., Brennan, K., Mulfaul, K., Ozaki, E., et al. (2015).

IL-18 Immunotherapy for Neovascular AMD: Tolerability and Efficacy in Nonhuman Primates.

Investigative Ophthalmology & Visual Science, 56(9), 5424–7.

• Nagai, N., Lundh von Leithner, P., Izumi-Nagai, K., Hosking, B., Chang, B., Hurd, R., et al. (2014).

Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis

and late-stage focal edema, neural cell loss, and dysfunction. Investigative Ophthalmology & Visual

Science, 55(6), 3709–3719.

• Tanimoto, N., Sothilingam, V., & Seeliger, M. W. (2012). Functional Phenotyping of Mouse Models

with ERG. (Vol. 935, pp. 69–78). Totowa, NJ: Humana Press.

• Nusinowitz, Steven, et al. "Electrophysiological testing of the mouse visual system." Systematic

Evaluation of the Mouse Eye: Anatomy, Pathology, and Biomethods (2002): 320-344.

Thank you!

• Prof. Dave Shima

• Peter Lundh von Leithner

• Dr Vivian Lee

• Dr Brett Hoskins

• Dr Ewa Kubala

• Joanna Holeniewska

• Shannon Conder

Peter Lundh von Leithner, PhD

Peter.lundh@ucl.ac.uk

Christine van Hover, PhD

cvanhover@phoenixreslabs.com

Thank You!If you have questions for the presenters please contact them by email.

For additional information on the solutions presented in this webinar please visit:

http://phoenixreslabs.com