measuring retinal function with erg in rodents
TRANSCRIPT
Measuring Retinal Function with ERG in Rodents
Sponsored by:
Christine van Hover, PhD.
Field Application Specialist,Phoenix Research Labs
Peter Lundh von Leithner, PhD.
Research Scientist,University College London
InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in
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Thank you to our event sponsor
Copyright 2016 Phoenix Research Labs and InsideScientific. All Rights Reserved.
Christine van Hover, PhD.Field Application Specialist,Phoenix Research Labs
Sponsored by:
Measuring Retinal Function with ERG in Rodents
Phoenix Research Labs Electroretinography
What is ERG?
Measuring the function of retinal cells by detecting their electrical activity in response to flashes of light
Phoenix has developed technology to enable a four step process
1. Prepare animal
2. Establish corneal contact
3. Acquire data
4. Analyze and present data
Ganzfeld ERG Focal ERG
• Dark lab technology enables easy set up and dark adaptation
• Needle electrodes are easy to place and secure
• ERG systems specifically designed for rodent eyes
1. Prepare Animal
• Near infrared light allows image-guided alignment with solid contact electrode
• Unique Maxwellian view allows uniform illumination of the eye
• Live chart mode shows real time coupling with the cornea
2. Establish corneal contact
2. Establish corneal contact
• Near infrared light allows image-guided alignment with solid contact electrode
• Unique Maxwellian view allows uniform illumination of the eye
• Live chart mode shows real time coupling with the cornea
3. Acquire Data
Before
After
• Simple interface to select protocol and collect data
• Clean algorithm removes 50 Hz or 60 Hz noise
• Allows detection of small differences
• Fewer light flashes
• No Faraday cage needed
• LabScribe calculates A and B waves
• Export graph and table of results
• Oscillatory potentials can be examined separately
• Automatic waterfall provides effective review
4. Analyze and present data
4. Analyze and present data
• LabScribe calculates A and B waves
• Export graph and table of results
• Oscillatory potentials can be examined separately
• Automatic waterfall provides effective review
Copyright 2016 P. Lundh von Leithner, Phoenix Research Labs and InsideScientific. All Rights Reserved.
Peter Lundh von Leithner, PhDResearch Scientist,University College London
Measuring Retinal Function with ERG in Rodents
Measuring Retinal Function using
focal ERG in Rodents
1. Who we are and what we do
2. How we generate Electroretinography (ERG) data
3. How we evaluate ERG data sets in the context ofpharmacological assays
4. Study examples
Institute of Ophthalmology UCLgoo.gl/q5zEbw
Moorfields Eye Hospitaltinyurl.com/hmz7um9
University College Londongoo.gl/gy5f
Translational Vision Research (TVR) Lab
• Professor Dave Shima
• Identify novel functions of vascular
growth factors (VGF and their
potential efficacy as therapeutic
targets
• VEGF role in neuron migration in
development and as a
neuroprotectant in the adult retina
• Present efforts are focused on
determine the neurorotectiove roles of
VGF in models of DR and Glaucoma
TVR In Vivo
• Fluorophotometry
• Perfusion assays
• Electroretinography
• Fluorescence funduscopy
• Scanning Laser Ophthalmoscopy
• Optical Coherence Tomography
Electroretinography (ERG) is a physiological assay
used to monitor retinal function of the retina. Specifically,
the function of the light-sensitive cells of the eye, the rods
and cones, and their connecting ganglion cells in the
retina are examined.
Preclinical electroretinography
• Developing physiological assays to assess the degree of retinal
function in models of vision
• Phenotyping of rodent models with including visual signal processing
• Monitoring sensory performance during and after therapeutic
interventions.
Amplification of signal
Animal Handling
Ketamine+Xylazine
Hypnovel+Domitor+Sublimaze
Isoflurane
Tropicamide 1%
Phenelypherine 1%
Photopic
Photopic
25
Stimulator settings
Scotopic
27
Scotopic
Micron Discover settings
28
31
33
fERGFFA
References
• Doyle, S. L., López, F. J., Celkova, L., Brennan, K., Mulfaul, K., Ozaki, E., et al. (2015).
IL-18 Immunotherapy for Neovascular AMD: Tolerability and Efficacy in Nonhuman Primates.
Investigative Ophthalmology & Visual Science, 56(9), 5424–7.
• Nagai, N., Lundh von Leithner, P., Izumi-Nagai, K., Hosking, B., Chang, B., Hurd, R., et al. (2014).
Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis
and late-stage focal edema, neural cell loss, and dysfunction. Investigative Ophthalmology & Visual
Science, 55(6), 3709–3719.
• Tanimoto, N., Sothilingam, V., & Seeliger, M. W. (2012). Functional Phenotyping of Mouse Models
with ERG. (Vol. 935, pp. 69–78). Totowa, NJ: Humana Press.
• Nusinowitz, Steven, et al. "Electrophysiological testing of the mouse visual system." Systematic
Evaluation of the Mouse Eye: Anatomy, Pathology, and Biomethods (2002): 320-344.
Thank you!
• Prof. Dave Shima
• Peter Lundh von Leithner
• Dr Vivian Lee
• Dr Brett Hoskins
• Dr Ewa Kubala
• Joanna Holeniewska
• Shannon Conder
Peter Lundh von Leithner, PhD
Christine van Hover, PhD
Thank You!If you have questions for the presenters please contact them by email.
For additional information on the solutions presented in this webinar please visit:
http://phoenixreslabs.com