life analytical chemistry-molecular imaging (mi): optical imaging
Post on 02-Jan-2016
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Life Analytical Chemistry-Molecular Imaging (MI): Optical Imaging
Gaolin Liang (梁高林) , Ph. D.Professor, Ph. D. AdvisorDeptartment of Chemistry
University of Science and Technology of China
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Optical Imaging
Fluorescence imaging
Non-Fluorescence-based optical imaging
Microscopic fluorescence imaging
Macroscopic fluorescence imaging
bioluminescence imaging
optical coherence tomography
photoacoustic microscopy
tissue spectroscopy
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Fluorescence imaging: Microscopic fluorescence imaging
History: In 1839, Rudolph Wagner visualized leukocytes rolling inblood vessels within membranous translucent tissues by using brightfieldTransillumination.
Nowadays: Several imaging approaches based on fluorescence microscopy that were established for visualizing cells in vitro have recently been adapted for in vivo imaging: multiphoton microscopy, laser-scanning confocal microscopy, fibre-optic approaches and spectrally encoded endoscopy.
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Fluorescence imaging: Macroscopic fluorescence imaging
There are two main types of imaging approach: fluorescence reflectance and tomographic fluorescence.
FMT-CT/FMT-CT fusion
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Optical Imaging
Fluorescence imaging
Non-Fluorescence-based optical imaging
Microscopic fluorescence imaging
Macroscopic fluorescence imaging
bioluminescence imaging
optical coherence tomography
photoacoustic microscopy
tissue spectroscopy
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Non-Fluorescence-based optical imaging: bioluminescence imaging
luciferase–luciferin pairs
firefly (Photinus pyralis) luciferase–luciferin
Renilla reniformis luciferase–coelenterazine
Gaussia princeps luciferase–coelenterazine
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Non-Fluorescence-based optical imaging: bioluminescence imaging
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Non-Fluorescence-based optical imaging: optical coherence tomography
Optical coherence tomography is based on light scattering and can beused to image microscopic structures in vivo (at a resolution of 2–15 μmand to a depth of 1–3 mm)
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Non-Fluorescence-based optical imaging: photoacoustic microscopy
Photoacoustic microscopy uses short laser pulses to irradiate tissueand temporarily raise its temperature (by millikelvins). Thermo-elasticexpansion then causes the emission of photoacoustic waves that canbe measured by wide-band ultrasonic transducers, offering improveddepth resolution in the 3–20 mm range
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Non-Fluorescence-based optical imaging: photoacoustic microscopy
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Non-Fluorescence-based optical imaging: photoacoustic microscopy
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Non-Fluorescence-based optical imaging: photoacoustic microscopy
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Non-Fluorescence-based optical imaging: photoacoustic microscopy
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Non-Fluorescence-based optical imaging: photoacoustic microscopy
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Non-Fluorescence-based optical imaging: tissue spectroscopy
tissue spectroscopy detects relative changes in the way in whichlight interacts with tissue and has been used extensively to improve earlydetection of gastrointestinal malignancies
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