let your lc inlet do the work - waters … your lc inlet do the work for your nano- to microscale...
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[ ACQUITY UPLC M-CLASS SYSTEM ]
LET YOUR LC INLET DO THE WORK FOR YOUR NANO- TO MICROSCALE SEPARATIONS
Analytical scientists are constantly
challenged to increase the sensitivity
of their LC/MS analyses to meet the
demanding goals of their laboratories.
In order to achieve this, focus is typically
directed toward improving the sensitivity
of the mass spectrometer or decreasing
the diameter of the LC column. Adapting
conventional separations instruments can
cause problems with reproducibility and
reliability. These questions of usability
prevent many scientists from taking
full advantage of nano- to microscale
separations in their methods.
Over the past 10 years UPLC® Technology
has continued to provide improved
chromatographic performance.
Now, with Waters’ newest addition, the
holistically designed ACQUITY UPLC®
M-Class System, nano- to microscale
separations will never be the same.
The ACQUITY UPLC M-Class System is
the most flexible nano- to microscale
instrument available to deliver UPLC-
quality separations across a wide range
of column dimensions, providing the
sensitivity required for an array of
applications. The system provides excellent
performance over the range of column
sizes, from 75 μm to 1.0 mm, delivering
outstanding results over a 170-fold
range of column volumes.
IF YOU’RE NOT T RYING TO INC REASE SENSIT IV IT Y BY UPGRADING YOUR LC INLET, YOU HAV E NO IDEA W HAT YOU’V E BEEN MISSING
desiredthe analyses you always
As a member of the ACQUITY UPLC
family, holistic design and intuitive
diagnostics make the ACQUITY UPLC
M-Class System the most robust,
reliable, and flexible UPLC system for
nano- to microscale separations.
With unprecedented usability, the
ACQUITY UPLC M-Class System
enables laboratories to analyze
compounds in low concentrations
amid complex matrices.
Confidently achieve your laboratory
goals with high-efficiency ACQUITY UPLC
M-Class Columns ranging from 75 µm
to 1.0 mm I.D. Use complementary mass
spectrometry to support our full range of
column I.D.’s, or run 300 µm to 1.0 mm
columns with optical detection.
[ ACQUITY UPLC M-CLASS SYSTEM ]
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ESI+ MSMicro-probe9 μL/min
CaffeineLidocaineSulfadimethoxineTerfenadineDanazol
BEH, 300 µm x 150 mm 5% to 95%B14 μL/min
14,000 psiACQUITY UPLC M-Class
8,000 psinanoACQUITY UPLC
Achieve faster separations using higher flow rates.
In this small molecule analysis the ACQUITY UPLC M-Class System provides a faster separation by approximately 30% when compared to the nanoACQUITY UPLC System.
T HE MOST ROBUST, RELIABLE, HIGHEST PERFORMING UPLC SYST EM FOR NANO- TO MIC ROSCALE SEPARAT IONS
■■ Higher sensitivity for any MS
■■ Maximum amount of data
from any sample
■■ Potential for less sample
preparation volume and cost
■■ Reduced solvent consumption
■■ Measure small amounts of analyte
in complex matrices with 2D
■■ Flexible configurations,
scalability, and confidence
for your laboratory
Run a wider range of column dimensions
for wide-ranging applications
desired
The ACQUITY UPLC M-Class System is
the product of years of Waters’ resolute
commitment to providing a reliable
nano- to microscale system. The low
system volume and newly redesigned
fluidics minimize dispersive and
adsorptive losses, ensuring superior
chromatographic resolution through
efficient use of sub-2-µm particles.
The system features improved robust
fittings, columns, and valves to ensure
that your analyte is resolved and
delivered to your mass spectrometer
or optical detector for characterization
and quantification experiments.
The ACQUITY UPLC M-Class System
features a microscale ESI probe in
the ZSpray™ API interface to deliver
superior sensitivity. As column I.D.
is decreased, this micro-MS probe
reduces band-broadening to maintain
chromatographic integrity.
T HE ACQUIT Y UPLC M-CLASS SYST EM DELIV ERS:
confidentlysee morewith less
■■ Highest sensitivity
UPLC separations yield increased
analyte concentration for higher
UV and MS signal responses
■■ Maximum chromatographic
resolution
New 15k psi-capable
ACQUITY UPLC M-Class
Columns deliver sub-2-µm
particle performance
■■ Faster nano- and
microscale separations
Increased operating pressures
yield maximum flexibility for
higher throughput analyses
■■ Higher resolution with
longer columns
Increased pressure enables
more separation power
■■ Confidence in sample analysis
New non-reactive wetted surfaces
throughout the fluidic path minimize
absorptive losses
■■ Increased solvent compatibility
Improved range of solvent
blends precisely metered
for gradient fidelity
■■ Increased sample capacity
Sample organizer compatibility
allows you to store a wide variety
of plates and vials in the system
for longer, unattended operation
■■ UV compatibility
UV flow cells compatible with
300 µm to 1.0 mm I.D. columns to
augment your UPLC/MS analysis
■■ Scalability
With flow rates from 200 nL/min
to 100 μL/min, achieve the best
chromatographic performance,
from 75 µm to 1.0 mm I.D. columns
covering a 170-fold range of
column volumes
■■ Increased flexibility
More valve configurations
enable a variety of applications,
increased throughput, and
maximized peak capacity
[ ACQUITY UPLC M-CLASS SYSTEM ]
The most reliable and
highest performing
nano- to microscale
instrument available
to deliver true UPLC
separations across the
widest range of sample
and column sizes.
■■ Maximum reproducibility
Direct flow control
■■ Interactive on-board
diagnostics
Effectively monitor
system performance
■■ MassLynx® Software
Fully compatible with
the system and the
suite of data processing
Application Managers
ACQUITY UPLC M-Class System with ACQUITY TUV Detector.
innovations that provide flexibility
Engineered for the lowest dispersion –
including an improved ESI micro-probe
design – and with an extended pressure
envelope, complex separations can
be accelerated without compromising
chromatographic fidelity.
The ACQUITY UPLC M-Class System is the
result of years of Waters’ experience and
research and delivers the promise of
robust nano- to microscale separations.
It was designed to meet the expanding
needs of researchers – every aspect
of the system is directed toward
increasing information.
Optical and MS detection
Optical detection grants an additional
identification and quantification tool
orthogonal to mass spectrometry.
The ACQUITY UPLC M-Class System
maximizes peak capacity, extending
the dynamic range of your analysis.
The ACQUITY UPLC M-Class System can
be easily combined with all types of mass
spectrometers, whether analyzing complex
samples in wide-ranging matrices with
a high resolution MS, or quantifying
peptides, metabolites, or other targeted
experiments with tandem MS.
Separation of small molecule standards on 1.0 mm I.D. (left) and 300 µm I.D. (right) columns with the ACQUITY TUV Detector, 100 nL flow cell. The 1.0 mm and the 300 µm I.D. columns provide comparable chromatographic resolution with a scaled sample load.
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1.0e-1
1.2e-1
1.4e-1
1.6e-1
1.8e-1
2.0e-1
2.2e-1
2.4e-1
2.6e-1
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3.0e-1
3.2e-1
3.4e-1
Gradient slope: 10% per CV5-95%B linear A= water/0.1% formic acidB= acetonitrile/0.1% formic acidλ 254ACQUITY UPLC M-Class BEH C18, 1.7 µm, 1.0 mm x 150 mm Column
4.0 ng of each injected:2-AcetylfuranAcetanilideAcetophenonePropylphenoneButyl ParabenBenzophenoneValerophenone
Time-0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00
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4.0e-2
6.0e-2
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1.6e-1
1.8e-1
2.0e-1
2.2e-1
Gradient slope: 10% per CV5-95%B linear A= water/0.1% formic acidB= acetonitrile/0.1% formic acid λ 254ACQUITY UPLC M-Class BEH C18, 1.7 µm, 300 µm x 150 mm Column
0.4 ng of each injected:2-Acetylfuran AcetanilideAcetophenonePropylphenoneButyl ParabenBenzophenoneValerophenone
[ ACQUITY UPLC M-CLASS SYSTEM ]
Because of its advanced fluidic pathways,
the ACQUITY UPLC M-Class System
delivers highly concentrated analyte
to the MS.
Maximize recovery
The non-reactive flowpath, unique
to the ACQUITY UPLC M-Class System,
ensures recovery of biologically
important molecules.
[ ACQUITY UPLC M-CLASS SYSTEM ]
Sample integrity.
Changes in phosphorylation can correlate to physiological changes and disease states. The recovery of multiple phosphorylated peptides, demonstrated here, addresses concerns of researchers who fear missed data could lead to poor quality decisions.
Sample: The MassPREP™ Enolase Digestion (p/n 186002325) with four added yeast enolase phosphopeptides (three are singly phosphorylated and one is doubly phosphorylated) – T18p, T19p, T43p, T43pp. The system design is verified with the appropriate standard to demonstrate successful resolution.Time
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100 13.25
14.40
18.1316.32 18.82
18.24
17.9215.32
20.09 35.3122.6521.42 24.9123.33 25.91 31.3428.3626.61 30.51 31.9932.90 37.26
19.17
18.79
18.2414.6313.2511.57 17.0616.30
21.4320.09
19.0824.86
22.6537.95
37.7625.38 36.9435.2733.9138.94
6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00
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T19 1P
T18 1P
T43 2P
T43 1P
COMP REHENSIV E 2D – EX PANDING T HE IMPACT
T HE FLEXIBLE DESIGN OF THE ACQUIT Y UPLC M-CLASS SYST EM OFFERS:
Until recently, 2D-LC was considered
both time-consuming and difficult to
accomplish. The ACQUITY UPLC M-Class
System has streamlined the 2D-LC
separation process with highly intuitive
menu-driven method setup, standardized
separation chemistries, and intelligent
valve operation.
Expanding on the use of a novel 2D
reversed phase/reversed phase (RP/RP)
approach, where the 1st dimension is
run at pH 10 and the 2nd at pH 2,
grants the ability to robustly increase
sample throughput.
The 2D configuration extends the dynamic
range of the analysis and yields itself to
host cell protein (HCP) analysis – removing
the need for cumbersome ELISA analysis.
0.0E+01.6E+6
0.0E+08.0E+5
0.0E+09.0E+5
0.0E+0
0.0E+0
5.0E+5
4.0E+5
Inte
nsity
0 5 10 15 20 25 30 min
Fraction 1
Fraction 2
Fraction 3
Fraction 4
Fraction 5
Separation of a MassPREP Digest Standard Mix 1 using a five step elution profile.
■■ Smaller I.D. columns for
increased sensitivity with
minimal sample use
■■ Optical detection with
ACQUITY® TUV and
PDA Detectors
■■ Optional non-reactive
flow paths
■■ Microscale electrospray
(ESI) probe for a ZSpray
API interface
■■ Comprehensive 2D analysis
■■ HDX capability
The ACQUITY UPLC M-Class System for 2D analysis.
[ ACQUITY UPLC M-CLASS SYSTEM ][ ACQUITY UPLC M-CLASS SYSTEM ]
With the increase in data quantity and quality along with improvements in DynamX HDX Data Analysis Software, researchers are in the best position to unlock the meaning of their data and improve decisions.
Butterfly plot comparing the deuterium uptake between two different states: bound (bottom) vs. unbound (top).
Expanding on the initial collaboration with
thought leaders worldwide, Waters has
extended the HDX capabilities of the
ACQUITY UPLC M-Class System. The higher
pressure envelope enables even faster
chromatographic resolution of peptic peptides.
The system integrates all the steps of
HDX from sample prep to actual sequence
determination, deuterium uptake curves, and
heat maps with the innovative DynamX™ HDX
Data Analysis Software.
Maximum chromatographic resolution
The ACQUITY UPLC M-Class System
enables columns packed with sub-2-µm
particles to improve nano- to microscale
separations. The Enzymate™ BEH Pepsin
Column adds a new level of control to
the entire HDX experiment.
MONITOR P ROT EIN CONFORMATION WIT H HDX T ECHNOLOGY
Be Assured. Choose Waters Global Services.
Waters Global Services helps
customers optimize laboratory
operations by providing superior
service, support, upgrades,
training, and Waters Quality Parts.®
For more information, go to
www.waters.com/services.
The ACQUITY UPLC M-Class System is designed for any nano- to
microscale separation you need to run. In addition Waters offers 75 µm
fused silica capillary columns, 150 µm iKeys, 300 µm stainless steel
columns, and access to traditional 1.0 mm I.D. columns. Depending on
your laboratory needs, we have an option for you.
ACQUITY UPLC M-CLASS COLUMNS
ACQUITY UPLC M-Class Columns are specifically
engineered for use with the ACQUITY UPLC M-Class
System and are designed for the high peak capacity
required for sample-limited applications. With 10 years
of UPLC sub-2-µm column design, these new columns
continue a tradition of reliability and performance.
of nano- to microscale chemistries
full range
iK EY SEPARAT ION DEV IC E
Ongoing collaboration with customers
uncovered a need for simplified microscale analysis.
The iKey™ Separation Device, with its ionKey™ Source,
is specifically designed to address this need, providing high
sensitivity separations to users doing routine analysis.
Its 150 μm I.D. provides optimum sensitivity and instrument
duty cycle.
[ ACQUITY UPLC M-CLASS SYSTEM ]
The ACQUITY UPLC M-Class System and SYNAPT G2-Si delivers high peak capacity separations with exceptional LC/MS data quality.
Time (min)
Peak capacity = 578 1 µg of a tryptic-digested50 kDa protein
75 µm x 150 mm CSH130 C18, 1.7 µm
55 min
The ACQUITY UPLC M-Class System
expands the performance of any nano-
to microscale MS analysis. It increases
the linear dynamic range of your analysis,
resulting in better MS analyses by
DDA or MS.E
The direct (non split) automatic solvent flow
control algorithms of the ACQUITY UPLC
M-Class System provide pulse-less flow for
increased retention time reproducibility.
Sample integrity is maintained due to
the ACQUITY UPLC M-Class System’s
non-reactive surfaces. New drivers for
MassLynx Software integrate the system
seamlessly into your existing workflows.
yourinlet to MS
Intuitive system-level diagnostics
Enables interactive display of system fluidic status to ensure system integrity and maximum performance – especially when changing columns, tubings, and fittings.
ACQUITY UPLC M-Class System with SYNAPT® G2-Si.
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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 508 478 2000 F: 508 872 1990 www.waters.com
www.waters.com/mclass
www.waters.com/mclasscolumns
Waters, The Science of What’s Possible, ACQUITY, ACQUITY UPLC, UPLC, MassLynx, Symmetry, SYNAPT, and Waters Quality Parts are registered trademarks of Waters Corporation. DynamX, Enzymate, ionKey, iKey, MassPREP, and ZSpray are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.
©2014 Waters Corporation. Printed in the U.S.A.March 2014 720004841EN LM-SIG
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