laser-assisted thermal-expansion microinjector

Unable to use fine tips under 0.5 μm for injection

Unable to inject into the cell having rigid cell wall such as plant cells

Complicated flow path brings on a time-consuming preparation

Conventional Microinjector

Inject by oil pressure or gas pressure If the capillary with less than 0.5 μm tip is used, the capillary itself will be moved forward due to the pressure. With complicated flow path

LTM-1000 : Structure

Feature

Extremely-high pressure (approx. 1MPa per 1Extremely-high pressure (approx. 1MPa per 1℃℃) )

makes it possible to use capillaries with the tip makes it possible to use capillaries with the tip

around 0.1-0.5μm without clogging.around 0.1-0.5μm without clogging. Simple flow path allows to start experiments Simple flow path allows to start experiments

right away saving a lot of labor.right away saving a lot of labor.

Heart-induced expansion of liquid in a sealed capillary generates high pressure.

LTM-1000

Applications

Injection of antibody into plant cells

Injection into chloroplasts of Cyanidioschyzon merolae

Chloroplast

Alexa 488

Lucifer Yellow

20µm

5µm

Alexa 546-dextran 10KAlexa 546-dextran 10K

50µm

50µm

ConventionalMethod LTM-1000

*Whereas the damage and sample escape appear by the conventional method, LTM-1000 ensures no damage and continuous injections without sample escape by using a fine tip capillary.

Injection into the female gametophyte of plant