isolation
Post on 31-Dec-2015
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DESCRIPTION
MATERIAL. Primary tumor. Cell line. Mouse xenograft. CD44 + /CD24 -/low /lin - ALDEFLUOR assay Side population. CD133? CD61? CD49f/CD29?. R1. R1. R2. R2. ISOLATION. FACS analysis. in vitro assay. in vivo assay. PROPERTIES. Tumorigenicity assay - PowerPoint PPT Presentation
TRANSCRIPT
ISOLATION
R2R1
FACS analysis
CD44+/CD24-/low/lin-
ALDEFLUOR assay
Side population
CD133?
CD61?
CD49f/CD29?
MATERIAL
Primary tumor Cell line Mouse xenograft
PROPERTIES
in vitro assay
Tumorsphere formation
Serial passages (self-renewal)
in vivo assay
R2R1
Tumorigenicity assay
Recapitulation of the initial phenotype
Serial passages (self-renewal)
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