impact of water deficit and nematode infection on growth...

Impact of Water Deficit and Nematode infection on Growth

and Physiological Responses of Mungbean (Vigna radiata L.)

Areej A. Alzarqaa, Shahira S. Roushdy, Ali A. Alderfasi,

Fahad A. AL-Yahya and Ahmed A. Dawabah

Sustainable Irrigation 2014

Thursday 19 June

Ali Alderfasi

King Saud University

Introduction

Water scarcity is being increasingly accepted as a major limitation for increased agricultural production and food security in the 21 st

century.

Water on a global scale is plentiful but 97 % is saline and 2.25 % is trapped in glaciers and ice, leaving only 0.75 % available in fresh water .

Drought and Disease(abiotic & biotic) stresses are global problem reducing plant growth and productivity worldwide.

Sustainable agricultural development, in arid and semi-arid regions is influenced to a great extent by available water and free of attacked of disease that might help effectively in developing agriculture.

Mungbean is a summer legume crop that has short- growth season and nitrogen fixation capability, also it was found as a drought resistance (Subtitute to Alfaalfa and barely froge).

Ali Alderfasi

King Saud University

To solve problem of drought , some strategies have been used:

1. Selecting drought-tolerant genotypes

2. Improving water use efficiency in plants.

3. Using suitable irrigation scheduling methods providing plants with enough water requirements.

4. Using municipal wastewater treatment plant.

5. Using some alternative crops such as a triticale complement of wheat.

6. Imported new genotypes have short growing season and drought resistance.(my presentation today will be on this topic).

Ali AlderfasiKing Saud University

Research Group Goals

Thus, the present study was proposed

to clarification of the strategy #6 and

to study the Physiological responses

of mungbean (Vigna radiate) to water

deficit and Nematode infection stress (This work is a part of master thesis of graduate student Areej Alzarqaa).

Ali AlderfasiKing Saud University

The Main Objective

How To Handel The Problem

Ali AlderfasiKing Saud University

● Plant Materials

Two genotypes of mungbean; Kawmay -1 and VC-2010 were tested in pots experiment.

● Drought Stress Induction

Three irrigation levels (20%, 40% and 80%) based on field capacity were used.

● Root-Knot Nematode Inoculum

After four weeks from sowing, half of the pots were inoculated with root-knot nematode @15000 egg/pot while other half was kept non- inoculated as control.

Ali Alderfasi King Saud University

● Experimental Design

The experiment has 12 treatments with 3 replicates (2x3x2x3), 36 experimental units

● Statistical analysis

Data were statistically analyzed according to the methods

described by Gomez and Gomez [1984]. The means were

compared using the least significant difference LSD.

RESULTS

Ali Alderfasi King Saud University

Ali Alderfasi King Saud University

0

5

10

15

20

25

30

35

40

45

50

80%

40%

20%

Kamway-1 VC-2010

Plant leaf area (cm2/plant) as affected by water deficit and Nematode infection

Noninfected Infected Noninfected Infected

Ali Alderfasi King Saud University

0

10

20

30

40

50

60

70

80

90

80%

40%

20%

Kamway-1 VC-2010

Stomata conductance (µmol/m2/s) as affected by water deficit and Nematode infection

Shoot water content (%) as affected by water deficit and nematode infection

69

70

71

72

73

74

75

76

77

80%

40%

20%

Kawmay-1 VC-2010

Shoot dry weight (g) as affected by water deficit and nematode infection

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

80%

40%

20%

VC-2010Kawmay-1

Leaf chlorophyll (a) content as affected by water deficit and Nematode infection

100

400

700

1000

1300

1600

1900

2200

2500

Chlorophyll a (µg/g)

80%

40%

20%

Noninfected Infected Noninfected Infected

Kawmay-1 VC2010

Ali Alderfasi King Saud University

20

120

220

320

420

520

Chlorophyll b (µg/g)

80%

40%

20%

VC-2010

Noninfected Infected Noninfected Infected

Kawmay-1

Leaf chlorophyll (b) content as affected by water deficit and Nematode infection

100

150

200

250

300

350

400

450

80 40 2080 40 20

Water capacity %

Non-infected

Infected

Vc-2010Kawmy1

Leaf proline content (mg/100g) as affected by water deficit and Nematode infection

Leaf protein content as affected by water deficit and Nematode

infection

ACKNOWLEDGMENT

THE CONFERENCE SECRETARIAT

Belinda Lopez

SPONSORSWIT Transactions on Ecology and the Environment and International Journal of Sustainable Development and

Planning

ORGANIZERS

Wessex Institute of Technology - UK , University of Lethbridge, Canada and University of South Australlia, Australlia

I would like to thank king Saud University for given me this opportunity to attend this conference and my thanks extend to:

Ali Alderfasi King Saud University

My pleasure to answer your questions