identifying drug resistant bacteria from a nasal swab 1.label the worksheet with the number on your...

Identifying drug resistant bacteria from a nasal swab

1. Label the worksheet with the number on your plate (1 - 5).2. Label each of the circles with the initials on the antibiotic

disk.3. Draw a picture of the zone of inhibition around each circle4. Measure the diameter of the zone of inhibition in mm and

then record it on in the table.

Antimicrobial agent

DoseDiameter of zone of inhibition

Ampicillin (AM) 10µg

Clindamycin (CM) 2µg

Erythromycin (E) 15µg

Gentamicin (GM) 10µg

Cefoxitin (FOX) 30µg

Vancomycin (VA) 30µg

Identifying drug resistant bacteria from a nasal swab

1. Find the other people that worked on the same plate as you (1-5)

2. Enter all your values for the diameter of the zone of inhibition.

3. Find the average diameter.

4. Enter it on the table

Identifying drug resistant bacteria from a nasal swabPlate 1

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Identifying drug resistant bacteria from a nasal swabPlate 2

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Identifying drug resistant bacteria from a nasal swabPlate 3

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Identifying drug resistant bacteria from a nasal swabPlate 4

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Identifying drug resistant bacteria from a nasal swabPlate 5

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Is your bacteria drug resistant? (Plate1 - Staph Aureus)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Is your bacteria drug resistant? (Plate 2 - Staph Aureus)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Is your bacteria drug resistant? (Plate 3 - Staph. Aureus)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Is your bacteria drug resistant? (Plate 4 - Staph. epididimis)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Is your bacteria drug resistant? (Plate 5 - staph epididimis)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Is Staph Aureus drug resistant?

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Is Staph Epididimis drug resistant?

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12