identifying drug resistant bacteria from a nasal swab 1.label the worksheet with the number on your...

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Identifying drug resistant bacteria from a nasal swab 1. Label the worksheet with the number on your plate (1 - 5). 2. Label each of the circles with the initials on the antibiotic disk. 3. Draw a picture of the zone of inhibition around each circle 4. Measure the diameter of the zone of inhibition in mm and then record it on in the table. Antimicrobial agent Dose Diameter of zone of inhibition Ampicillin (AM) 10µg Clindamycin (CM) 2µg Erythromycin (E) 15µg Gentamicin (GM) 10µg Cefoxitin (FOX) 30µg Vancomycin (VA) 30µg

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Page 1: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swab

1. Label the worksheet with the number on your plate (1 - 5).2. Label each of the circles with the initials on the antibiotic

disk.3. Draw a picture of the zone of inhibition around each circle4. Measure the diameter of the zone of inhibition in mm and

then record it on in the table.

Antimicrobial agent

DoseDiameter of zone of inhibition

Ampicillin (AM) 10µg

Clindamycin (CM) 2µg

Erythromycin (E) 15µg

Gentamicin (GM) 10µg

Cefoxitin (FOX) 30µg

Vancomycin (VA) 30µg

Page 2: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swab

1. Find the other people that worked on the same plate as you (1-5)

2. Enter all your values for the diameter of the zone of inhibition.

3. Find the average diameter.

4. Enter it on the table

Page 3: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swabPlate 1

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Page 4: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swabPlate 2

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Page 5: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swabPlate 3

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Page 6: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swabPlate 4

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Page 7: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Identifying drug resistant bacteria from a nasal swabPlate 5

Antimicrobial agent

DoseDiameter of zone of inhibition

Average diameter

Ampicillin (AM) 10µg

1.

2.

3.

4.

5.

Clindamycin (CM) 2µg

1.

2.

3.

4.

5.

Erythromycin (E) 15µg

1.

2.

3.

4.

5.

Gentamicin (GM) 10µg

1.

2.

3.

4.

5.

Cefoxitin (FOX) 30µg

1.

2.

3.

4.

5.

Vancomycin (VA) 30µg

1.

2.

3.

4.

5.

Page 8: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is your bacteria drug resistant? (Plate1 - Staph Aureus)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Page 9: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is your bacteria drug resistant? (Plate 2 - Staph Aureus)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Page 10: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is your bacteria drug resistant? (Plate 3 - Staph. Aureus)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Page 11: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is your bacteria drug resistant? (Plate 4 - Staph. epididimis)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Page 12: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is your bacteria drug resistant? (Plate 5 - staph epididimis)

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Page 13: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is Staph Aureus drug resistant?

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12

Page 14: Identifying drug resistant bacteria from a nasal swab 1.Label the worksheet with the number on your plate (1 - 5). 2.Label each of the circles with the

Is Staph Epididimis drug resistant?

Antimicrobial agent

Diameter of zone of inhibition

Resistant Intermediate Susceptible R, I or S

Ampicillin (AM)

10µg ≤ 13 N/a ≥ 17

Clindamycin (CM)

2µg ≤ 14 15-20 ≥ 21

Erythromycin (E)

15µg ≤ 13 14-22 ≥ 23

Gentamicin (GM)

10µg ≤ 12 13-14 ≥ 15

Cefoxitin (FOX)

30µg ≤ 21 N/a ≥ 22

Vancomycin (VA)

30µg ≤ 9 10-11 ≥ 12