hpp – shotgun status

HPP – Shotgun Status

• A.M.• 30/11/2012

Project 22/8

FDR 1%: Protein level

Peptides & proteins by labs

Proteins by experiment

Exclusive Proteins (including secretome)

Chr16 - distribution

Prot: 537Genes: 385

Exclusive proteins Chr16

FDR 1% at peptide level from 24 experiments

FDR 1% at peptide level from 24 experiments

Exclusive Proteins

Exclusive Proteins of chromosome 16

Protein increase with experiment number

Quantitative information?

Q = # peptides/kDa

Only for chromosome 16 proteins

Quantitative information?

5 Less abundant proteins of chromosome 16• CHD9_HUMAN Chromodomain-helicase-DNA-binding protein• CBP_HUMAN CREB-binding protein• Isoform 3 of Dynein heavy chain 3, axonemal• RBBP6_HUMAN Isoform 3 of E3 ubiquitin-protein ligase RBBP6• ZN646_HUMAN Zinc finger protein 646

5 Most abundant proteins of chromosome 16• RS2_HUMAN 40S ribosomal protein S2• RS15A_HUMAN 40S ribosomal protein S15a• COTL1_HUMAN Coactosin-like protein• ELOB_HUMAN Transcription elongation factor B polypeptide 2• ALDOA_HUMAN Fructose-bisphosphate aldolase A