hpp – shotgun status
DESCRIPTION
A.M. 30/11/2012. HPP – Shotgun Status. Project 22/8. FDR 1%: Protein level. Peptides & proteins by labs. Proteins by experiment. Exclusive Proteins ( including secretome ). Chr16 - distribution. Prot : 537 Genes: 385. Exclusive proteins Chr16. - PowerPoint PPT PresentationTRANSCRIPT
HPP – Shotgun Status
• A.M.• 30/11/2012
Project 22/8
FDR 1%: Protein level
Peptides & proteins by labs
Proteins by experiment
Exclusive Proteins (including secretome)
Chr16 - distribution
Prot: 537Genes: 385
Exclusive proteins Chr16
FDR 1% at peptide level from 24 experiments
FDR 1% at peptide level from 24 experiments
Exclusive Proteins
Exclusive Proteins of chromosome 16
Protein increase with experiment number
Quantitative information?
Q = # peptides/kDa
Only for chromosome 16 proteins
Quantitative information?
5 Less abundant proteins of chromosome 16• CHD9_HUMAN Chromodomain-helicase-DNA-binding protein• CBP_HUMAN CREB-binding protein• Isoform 3 of Dynein heavy chain 3, axonemal• RBBP6_HUMAN Isoform 3 of E3 ubiquitin-protein ligase RBBP6• ZN646_HUMAN Zinc finger protein 646
5 Most abundant proteins of chromosome 16• RS2_HUMAN 40S ribosomal protein S2• RS15A_HUMAN 40S ribosomal protein S15a• COTL1_HUMAN Coactosin-like protein• ELOB_HUMAN Transcription elongation factor B polypeptide 2• ALDOA_HUMAN Fructose-bisphosphate aldolase A