gregory schneider graphene bionanoscience:...

Gregory SCHNEIDER Graphene bionanoscience: from hydrophobicity to DNA sequencing

PhD at the University of Strasbourg,with Prof. Gero Decher, on nanoparticle

functionalisation using the Layer-by-layer technique with applications as drug

delivery vehicles for cancer therapy.

From 2006-2009, postdoc with Prof. George Whitesides at the Dept of

Chemistry and Chemical Biology at Harvard University on protein biophysics

and microfluidic devices

Currently senior postdoc with Prof. Cees Dekker at the Kavli Institute of

Nanoscience at TU Delft on graphene self-assembly and graphene nanopore

devices for genomic DNA sequencing.

He is looking forward to starting his own group at the frontier of rational

bioorganic self-assembly, microfluidics and graphene bionanoscience.

Graphene Bionanoscience From Hydrophobicity to Genomic DNA Sequencing

G.F. Schneider, g.f.schneider@tudelft.nl Cees Dekker group, Kavli Institute of Nanoscience, TU Delft, NL

Solid state nanopores

-Principle and drawbacks

Hydrophobic on hydrophilic

-Graphene wedging

Graphene nanopores

-Towards DNA Sequencing

Outline of this talk

DNA translocation through a nanopore Idea of the experiment:

For a review see: C. Dekker, Nature Nanotech. 2, 209 (2007)

Dreams of nanopore-based DNA sequencing

Advantages of nanopore sequencing:

•  single molecule •  no amplification •  label-free •  long read lengths •  electrical detection

Solid-state nanopores from silicon processing

5 nm

A. Storm et al, Nature Mater. 2, 537 (2003)

ssDNA/RNA through α-hemolysin membrane pores (Kasianowicz, Branton, Akeson, Bayley, Meller, …)

Blockades of polyU through α-hemolysin

A major drawback for sequencing: the thickness

30 nm thick SiN 0.3 nm/base

� Average of 100 bases

Genomic screening beyond mere DNA sequencing Scanning the position of proteins along DNA

Screening of local proteins along DNA, e.g., transcription factors, nucleosomes..

Use SINGLE-ATOM-LAYER-THIN graphene as the membrane for a nanopore

Postma, Nanoletters 2010

Graphene by mechanical exfoliation

Blake et al, APL, (2007)

Hydrophobic polymer

Hydrophilic substrate

‘Wedging transfer’

Schneider et al, Nano Letters 10, 1912, April 19, 2010

A few flakes The target

After transfer Polymer dissolved

Using wedging to transfer graphene

10 μ

Schneider et al, Nano Letters 10, 1912, April 19, 2010

10 μ 10 μ

Transfer of a graphene monolayer by wedging

Schneider et al, Nano Letters 10, 3163, July 7, 2010

Drill a nanopore with a focussed TEM beam

Schneider et al, Nano Letters 10, 3163, July 7, 2010

Translocation of dsDNA through graphene nanopores

Schneider et al, Nano Letters 10, 3163, July 7, 2010

Scatter diagram conductance blockade versus translocation time

Schneider et al, Nano Letters 10, 3163, July 7, 2010

Very recent work: Towards atomically precise graphene nanopores, using self repair at high temperature

Song, Schneider et al, submitted

Summing-up:

�  Solid-state and biological nanopores are thick

�  Wedging allows minute-graphene transfer

�  DNA translocate through pores in graphene

�  Self-repair permits graphene with crystaline edges

�  Current tunneling is next

Our group:

Stefan Kowalczyk Vlad Karas Koen Schuurbiers Kasper van Schie Okke Groen Stephanie Luik Xuyi Wang Cees Dekker

Collaborations:

Henny Zandbergen Bo Song Qiang Xu Gregory Pandraud Meng Yue Wu

Lieven Vandersypen Victor Calado Gilles Buchs