g.007 hardware open-cad

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G.007 Hardware open-CAD. Xenon Light. EM-CCD Camera. Fluidics. Autofocus. Objective. Stage. 3U Imaging 2U Sequencing. 30 msec move/settle 42 kbp/sec 10-60 Gbp/run $124,000. Hardware: Polonator G.007. Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics - PowerPoint PPT Presentation

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G.007 Hardware open-CAD

• 30 msec move/settle• 42 kbp/sec• 10-60 Gbp/run• $124,000

EM-CCD Camera

Xenon Light

Fluidics

Autofocus

Objective

Stage

3U Imaging2U Sequencing

Hardware: Polonator G.007Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics

Shendure, Porreca et al. 2005

Danaher, Hamamatsu, Leica $124K with computer & 1 yr serviceContact: kevin.mccarthy@danahermotion.com

Rich Terry et al. 2007

DIY

PersonalGenome.org

36 Flowcells G.007 60M beads per flowcell

PersonalGenome.orgFilling with beads

Gel array, 12M beadsShendure, Porreca et al. 2005

Glass array, 60M beadsKim, Porreca et al. 2007

1.6M reads 12M reads(M. Umbarger)

Higher density & accuracy

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