g.007 hardware open-cad

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G.007 Hardware open-CAD 30 msec move/settle 42 kbp/sec 10-60 Gbp/run • $124,000 EM-CCD Camera Xenon Light Fluidics Autofocus Objective Stage 3U Imaging 2U Sequencing

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G.007 Hardware open-CAD. Xenon Light. EM-CCD Camera. Fluidics. Autofocus. Objective. Stage. 3U Imaging 2U Sequencing. 30 msec move/settle 42 kbp/sec 10-60 Gbp/run $124,000. Hardware: Polonator G.007. Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics - PowerPoint PPT Presentation

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Page 1: G.007 Hardware open-CAD

G.007 Hardware open-CAD

• 30 msec move/settle• 42 kbp/sec• 10-60 Gbp/run• $124,000

EM-CCD Camera

Xenon Light

Fluidics

Autofocus

Objective

Stage

3U Imaging2U Sequencing

Page 2: G.007 Hardware open-CAD

Hardware: Polonator G.007Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics

Shendure, Porreca et al. 2005

Danaher, Hamamatsu, Leica $124K with computer & 1 yr serviceContact: [email protected]

Rich Terry et al. 2007

DIY

PersonalGenome.org

Page 3: G.007 Hardware open-CAD

36 Flowcells G.007 60M beads per flowcell

PersonalGenome.orgFilling with beads

Page 4: G.007 Hardware open-CAD

Gel array, 12M beadsShendure, Porreca et al. 2005

Glass array, 60M beadsKim, Porreca et al. 2007

1.6M reads 12M reads(M. Umbarger)

Higher density & accuracy