forensic science. serology blood typing abo blood typing

FORENSIC SCIENCE

Serology

Blood Typing ABO Blood Typing

Serology

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Serology

Presumptive vs. Confirmatory Testing Blood:

TMB : Tetramethylbenzidine + H2O2 -> blue/green

HemaTrace LMG Luminol BlueStar

Serology Gatekeeper of DNA analysis

Extraction Quantification Amplification Capillary Electrophoresis Data Analysis

Amplification

PCR = Polymerase Chain Reaction

1. Denature 2. Anneal3. Extend

Data Analysis

Single Source:

Data Analysis

Mixture:

Example

Hair

Cuticle Scales Cortex Medulla

Hair

Stages of hair growth:1. Anagen2. Catagen3. Telogen

Hair

Hair

Hair

Hair

Fingerprint Analysis

Fingerprint Analysis

Class characteristics

Fingerprint Analysis

Fingerprint Analysis

Individual Characteristics

Fingerprint Analysis

Collecting prints: Porous surfaces (anything that can absorb):

Ninhydrin : causes purple reaction DFO : glows when illuminated by light

Nonporous surfaces (things that don’t absorb): Powder (black and fluorescent) Pictures Cyanoacrylate (superglue fuming)

Example

1. Look at unknown1. Shape2. Minutia

2. Compare

Blood Spatter Analysis

Methods:-Angle of Impact-Direction of Travel-Stringing

Blood Spatter Analysis

Blood Spatter Analysis

Blood Spatter Analysis

Blood Spatter Analysis

Blood Spatter Analysis

Glass Analysis

-Concentric Cracks-Radial Fractures

Which came first? A or B?