final stuff: n lab practical: apr 29 n final exam: due fri may 7 @ 2:15
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Final stuff:Final stuff: Lab practical: Apr 29Lab practical: Apr 29
Final exam: due Fri May 7 @ Final exam: due Fri May 7 @ 2:152:15
TodayToday
IDEM samplingIDEM sampling
Assessment exerciseAssessment exercise
MACROINVERTEBRATE COMMUNITY
MONITORING EXAMPLE
INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
STAGES OF BIOLOGICAL COMMUNITY ASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING LABORATORY PROCESSING
SAMPLE COLLECTION
EcologicalIntegrity
What are We Trying to Measure ??
Water Quality
Habitat, Flow regime
Biological Interactions
“The ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of
organisms having a species composition, diversity, and functional organization
comparable to the best natural habitats within a region.”
BIOLOGICAL INTEGRITY
STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
Aquatic Larvae
Aquatic Adults
Terrestrial Adults
Adult and Larval Caddisflies
(Trichoptera)
USEPA Rapid Bioassessment Protocols
Riffle Habitats
Two Composite
KICK Screen Samples
STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
Sample Elutriation
Remove Gravel and Sand
Remove Excess CPOM
The Final Sample Ready for
Preservation
Field Preservation of Samples
GPS for Spatial Reference
PAPERWORK…PAPERWORK…. TRIPLE REDUNDANCY
Water Chemistry
Habitat Assessments
Field Observations
STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
All Samples are Returned to Laboratory for Processing
Squares (25 cmSquares (25 cm22))
Removed by Suction to Removed by Suction to Petri DishPetri Dish
100 Organism Sub-Sample
15 Minute Sort Sub-Sample
100 Organism Sub-Sample
Selected at Random
Second Sub-Sample 15 Minutes
Entire Pan “Randomly” Sorted
Using Forceps
Final Sort Under Microscope
Sub-Samples Processed to Sub-Samples Processed to Family LevelFamily Level
All Samples Preserved for Permanent Museum Storage
STAGES OF ASTAGES OF A BIOLOGICAL COMMUNITY BIOLOGICAL COMMUNITY
ASSESSMENTASSESSMENT
DATA REDUCTION AND ANALYSIS
FIELD PROCESSING
LABORATORY PROCESSING
SAMPLE COLLECTION
TEN SUB-METRICS
SITE INFORMATIO
NTAXONOMIC
INFORMATION
AND
TAXA COUNTS
LABORATORY BENCHSHEET
Example of 2 Sub-Metrics
E
P
T
EPT Index = 6 Families
EPT Count = 7 Individuals
Metric Scores = 0, 2, 4, 6, 8
SCORING CRITERIA FOR THE FAMILY LEVELMACROINVERTEBRATE INDEX OF BIOTIC INTEGRITY
(mIBI)USING PENTASECTION AND CENTRAL TENDENCY ON THE
LOGARITHMIC TRANSFORMED DATA DISTRIBUTIONS OF THE 1990-1994 RIFFLE KICK SAMPLES
CLASSIFICATION SCORE
8 6 4 2 0
Family LevelHBI
4.08 4.09 - 4.54 4.55- 5.05 5.06 - 5.62 5.63
Number ofTaxa
18 17-15 14-11 10-8 7
Number ofIndividuals
350 349-213 212-130 129-80 79
PercentDominant
Taxa 22.1 22.2-31.1 31.2-43.8 43.9-61.5 61.6
EPTIndex
8 7-6 5-4 3 2
EPTCount
195 194-92 91-43 42-20 19
EPTCount To
TotalNumber ofIndividuals
0.69 0.68-0.47 0.46-0.30 0.29-0.14 0.13
EPT CountTo
ChironomidCount
11.66 11.65-5.71 5.70-2.56 2.55-0.89 0.88
ChironomidCount
6 7-19 20-54 55-146 147
TotalNumber ofIndividualsTo Numberof Squares
Sorted
410 409-172 171-72 71-30 29
Scoring Criteria
for Riffle KICK
Samples
Not All Rivers and Streams Have Riffle Habitats
Multi-Plate Samples
are Collected
IndividuallyMulti-Plate
Samples are Preserved in the Field and
Scraped in the Laboratory
Systematic Community Assessments of Benthic
Macroinvertebrate Communities are Effective
SUMMARY
IBI -- Family Level Multi-Metric Indices Provide:
Cost Effective Data of Ecological Health of Rivers and Streams
Assessment Information to Make
Management Decisions
Lammert & Allen 1999Lammert & Allen 1999Scoring Classification
Metric 1 3 5
Total Taxa < 22 23 - 30 > 30
Mayfly Taxa 0 - 3 4 - 6 > 6
Caddisfly Taxa 2 - 3 4 - 5 > 5
Dipteran Taxa 3 - 4 5 - 6 > 6
Relative Abundance of
Omnivores
70 – 100 % 57 – 69 % 43 – 56 %
Relative Abundance of
Filterers
51 – 69 % 50 – 31 % 9 – 30 %
Relative Abundance of
Grazers
0 – 19 % 20 – 32 % > 33 %
Relative Abundance of
Predators
24 – 33 % 14 – 23 % 2 – 13 %
Dominance 68 – 88 % 47 – 67 % 24 – 46 %
Actual Observation
IBI Score
(no.) (no.)
Total Taxa 19 1Mayfly Taxa 2 1Caddisfly Taxa 2 1Dipteran Taxa 3 1Relative Abundance of Omnivores 0.42 5Relative Abundance of Filterers 0.02 5Relative Abundance of Grazers 0.01 1Relative Abundance of Predators 0.43 1Dominance 0.30 5
B-IBI score Lammert & Allan (1999) Total IBI 21
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