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EFFICACY OF SUISENG® AND RHINISENG® WHEN COMBINED IN A SINGLE INJECTION IN GILTS
Camprodon A., Gibert X., Montané J., Perozo E., Bech G., March R., Maldonado J.
HIPRA, Avda La Selva, 135 - 17170 Amer (Girona) – SpainCorresponding author: agusti.camprodon@hipra.com
INTRODUCTION
aim of facilitating the management of a farm and reducing the number of injections applied to pigs.SUISENG® is a vaccine against neonatal diarrhoea in piglets and sudden death in sows, and RHINISENG® is a vaccine for preventing progressive and non-progressive atrophic rhinitis of swine. Both vaccines contain a similar adjuvant, Hipramune-G, and are administered, following the same vaccination schedule, to pregnant sows and gilts to passively protect their offspring:
SUISENG®
the heat- labile toxin (LT) of Escherichia coli, the β toxin of Clostridium perfringens type C and the α toxin of Clostridium novyi. RHINISENG® contains the recombined toxin of Pasteurella multocida type D and inactivated Bordetella bronchiseptica. The aim of this study is to compare serological responses of SUISENG® and RHINISENG® when they are mixed and injected in gilts, in comparison with the injection of these two vaccines separately.
MATERIALS AND METHODS
Twenty-four eight-month-old gilts, clinically healthy and free from antibodies against P. multocida toxin (PMT), B. bronchiseptica (Bb) and the main pathogenicity factors of E. coli were randomly assigned to 4 groups of 6 animals each.
The groups were vaccinated twice intramuscularly 3 weeks apart with SUISENG®+RHINISENG® mixed (4ml), or with SUISENG® alone (2ml) or with RHINISENG® alone (2ml). The control group received a 2ml dose of PBS twice as a placebo.
applied), 3 weeks later (when the second dose was applied) and 3 weeks afterwards. Serum samples were stored at -20ºC until testing. The serological methods used were: i) a commercially available ELISA assay for the detection of antibodies against P. multocida dermonecrotic toxin (Oxoid); ii) the micro-agglutination test for B. bronchiseptica; iii) and in-house ELISA assays for the detection of antibodies against relevant E. coli
RESULTS
Figure 1. Mean log3 (±SEM) anti-PMT antibody titre
Figure 2. Mean log2 (±SEM) micro-agglutinating titres for B. bronchiseptica
Figure 3.
and Mann.Whitney for two-by-two comparisons)
The rest of E. coli valences included in the vaccine followed a similar
CONCLUSIONS AND DISCUSSION
when comparing the combination of SUISENG® and RHINISENG® to the group of RHINISENG®
observed in the seroconversion against E. colithe combination of SUISENG® and RHINISENG® to the group of SUISENG® alone. These results indicate that SUISENG® and RHINISENG® can be combined and administered in a single injection to gilts and sows, obtaining a comparable serological response against all the antigens contained in the two vaccines.
Published in the 5 European Symposium of Porcine Health Management 2013th
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