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EFFICACY OF SUISENG ® AND RHINISENG ® WHEN COMBINED IN A SINGLE INJECTION IN GILTS Camprodon A., Gibert X., Montané J., Perozo E., Bech G., March R., Maldonado J. HIPRA, Avda La Selva, 135 - 17170 Amer (Girona) – Spain Corresponding author: [email protected] INTRODUCTION aim of facilitating the management of a farm and reducing the number of injections applied to pigs. SUISENG ® is a vaccine against neonatal diarrhoea in piglets and sudden death in sows, and RHINISENG ® is a vaccine for preventing progressive and non-progressive atrophic rhinitis of swine. Both vaccines contain a similar adjuvant, Hipramune-G, and are administered, following the same vaccination schedule, to pregnant sows and gilts to passively protect their offspring: SUISENG ® the heat- labile toxin (LT) of Escherichia coli , the β toxin of Clostridium perfringens type C and the α toxin of Clostridium novyi . RHINISENG ® contains the recombined toxin of Pasteurella multocida type D and inactivated Bordetella bronchiseptica . The aim of this study is to compare serological responses of SUISENG ® and RHINISENG ® when they are mixed and injected in gilts, in comparison with the injection of these two vaccines separately. MATERIALS AND METHODS Twenty-four eight-month-old gilts, clinically healthy and free from antibodies against P. multocida toxin (PMT), B. bronchiseptica (Bb) and the main pathogenicity factors of E. coli were randomly assigned to 4 groups of 6 animals each. The groups were vaccinated twice intramuscularly 3 weeks apart with SUISENG ® +RHINISENG ® mixed (4ml), or with SUISENG ® alone (2ml) or with RHINISENG ® alone (2ml). The control group received a 2ml dose of PBS twice as a placebo. applied), 3 weeks later (when the second dose was applied) and 3 weeks afterwards. Serum samples were stored at -20ºC until testing. The serological methods used were: i) a commercially available ELISA assay for the detection of antibodies against P. multocida dermonecrotic toxin (Oxoid); ii) the micro-agglutination test for B. bronchiseptica ; iii) and in- house ELISA assays for the detection of antibodies against relevant E. coli RESULTS Figure 1. Mean log3 (±SEM) anti-PMT antibody titre Figure 2. Mean log2 (±SEM) micro-agglutinating titres for B. bronchiseptica Figure 3. and Mann.Whitney for two-by-two comparisons) The rest of E. coli valences included in the vaccine followed a similar CONCLUSIONS AND DISCUSSION when comparing the combination of SUISENG ® and RHINISENG ® to the group of RHINISENG ® observed in the seroconversion against E. coli the combination of SUISENG ® and RHINISENG ® to the group of SUISENG ® alone. These results indicate that SUISENG ® and RHINISENG ® can be combined and administered in a single injection to gilts and sows, obtaining a comparable serological response against all the antigens contained in the two vaccines. Published in the 5 European Symposium of Porcine Health Management 2013 th

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EFFICACY OF SUISENG® AND RHINISENG® WHEN COMBINED IN A SINGLE INJECTION IN GILTS

Camprodon A., Gibert X., Montané J., Perozo E., Bech G., March R., Maldonado J.

HIPRA, Avda La Selva, 135 - 17170 Amer (Girona) – SpainCorresponding author: [email protected]

INTRODUCTION

aim of facilitating the management of a farm and reducing the number of injections applied to pigs.SUISENG® is a vaccine against neonatal diarrhoea in piglets and sudden death in sows, and RHINISENG® is a vaccine for preventing progressive and non-progressive atrophic rhinitis of swine. Both vaccines contain a similar adjuvant, Hipramune-G, and are administered, following the same vaccination schedule, to pregnant sows and gilts to passively protect their offspring:

SUISENG®

the heat- labile toxin (LT) of Escherichia coli, the β toxin of Clostridium perfringens type C and the α toxin of Clostridium novyi. RHINISENG® contains the recombined toxin of Pasteurella multocida type D and inactivated Bordetella bronchiseptica. The aim of this study is to compare serological responses of SUISENG® and RHINISENG® when they are mixed and injected in gilts, in comparison with the injection of these two vaccines separately.

MATERIALS AND METHODS

Twenty-four eight-month-old gilts, clinically healthy and free from antibodies against P. multocida toxin (PMT), B. bronchiseptica (Bb) and the main pathogenicity factors of E. coli were randomly assigned to 4 groups of 6 animals each.

The groups were vaccinated twice intramuscularly 3 weeks apart with SUISENG®+RHINISENG® mixed (4ml), or with SUISENG® alone (2ml) or with RHINISENG® alone (2ml). The control group received a 2ml dose of PBS twice as a placebo.

applied), 3 weeks later (when the second dose was applied) and 3 weeks afterwards. Serum samples were stored at -20ºC until testing. The serological methods used were: i) a commercially available ELISA assay for the detection of antibodies against P. multocida dermonecrotic toxin (Oxoid); ii) the micro-agglutination test for B. bronchiseptica; iii) and in-house ELISA assays for the detection of antibodies against relevant E. coli

RESULTS

Figure 1. Mean log3 (±SEM) anti-PMT antibody titre

Figure 2. Mean log2 (±SEM) micro-agglutinating titres for B. bronchiseptica

Figure 3.

and Mann.Whitney for two-by-two comparisons)

The rest of E. coli valences included in the vaccine followed a similar

CONCLUSIONS AND DISCUSSION

when comparing the combination of SUISENG® and RHINISENG® to the group of RHINISENG®

observed in the seroconversion against E. colithe combination of SUISENG® and RHINISENG® to the group of SUISENG® alone. These results indicate that SUISENG® and RHINISENG® can be combined and administered in a single injection to gilts and sows, obtaining a comparable serological response against all the antigens contained in the two vaccines.

Published in the 5 European Symposium of Porcine Health Management 2013th