comparison of some replication components

Enzyme Function

Prokaryotes Eukaryotes

Helicase

Primase

Polymerase

Proofreading

Sliding clamp

Prevent ss closure

DnaB

DnaG

Pol III

Pol III

sliding clamp

SSBPs

MCM

Primase s.u. of -polymerase

Pol and

Pol and

PCNA (Proliferating Cell Nuclear Antigen)

RPA (Replication Protein A)

Comparison of some replication components

A Problem and a Solution

Telomere Formation

A model for t loop formation

Regulation of Gene Expression in Regulation of Gene Expression in EukaryotesEukaryotes

(A)(A)nn

(A)(A)nn

(A)(A)nn

(A)(A)nn

Nucleus

mRNA

Protein

TranscriptiTranscriptionon

TranslatiTranslationonGenome Genome

OrganizatiOrganizationon

StabilityStability Post-Post-TranslatiTranslati

onon

7m7mGG7m7mGG7m7mGG7m7mGG

Reproductive cloning

Biotechnology/Cloning

Reproductive cloning

Therapeutic cloning

http://www.rndsystems.com/dam_public/6515.jpg

Therapeutic cloning

First Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”

Cloning Vectors: pBR322 plasmid

Cloning into pBR322

RT-PCR and Cloning

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Golden Rice

Weeding Out Land MinesFirst Glowing Pet