Enzyme Function

Prokaryotes Eukaryotes

Helicase

Primase

Polymerase

Proofreading

Sliding clamp

Prevent ss closure

DnaB

DnaG

Pol III

Pol III

sliding clamp

SSBPs

MCM

Primase s.u. of -polymerase

Pol and

Pol and

PCNA (Proliferating Cell Nuclear Antigen)

RPA (Replication Protein A)

Comparison of some replication components

A Problem and a Solution

Telomere Formation

A model for t loop formation

Regulation of Gene Expression in Regulation of Gene Expression in EukaryotesEukaryotes

(A)(A)nn

(A)(A)nn

(A)(A)nn

(A)(A)nn

Nucleus

mRNA

Protein

TranscriptiTranscriptionon

TranslatiTranslationonGenome Genome

OrganizatiOrganizationon

StabilityStability Post-Post-TranslatiTranslati

onon

7m7mGG7m7mGG7m7mGG7m7mGG

Reproductive cloning

Biotechnology/Cloning

Reproductive cloning

Therapeutic cloning

http://www.rndsystems.com/dam_public/6515.jpg

Therapeutic cloning

First Cloning Experiment

Cohen et al., 1973

“Recombinant DNA”

Cloning Vectors: pBR322 plasmid

Cloning into pBR322

RT-PCR and Cloning

Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615

“Supermice”

Golden Rice

Weeding Out Land MinesFirst Glowing Pet