chlorophyll a and phycocyanin from hyperspectral airborne...

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Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

Integra7veHAB

Approach

WaterIntakeLoca7ons

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

( " ( ( ( (( ( ( ( ( (

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Airborne(Resonon(Pika(II(

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( ( ( ( (

( ( ( ( ( ( ( (

  ( ( ( (  ( (

( ( ( (

  ( ( (  ( ( (  ( ( ( ( (

( ( ( (  ( ( ( ( ( (

(  ( ( ( (

( ( ( ( (

Hyperspectral(ocean(color(sensor(that(measures(water7leaving(spectral(radiance((and((sky(downwelling(irradiance.((

( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

( ( (( ( ( ( ( (

( ( ((( ( ( ( (

( ( (

( ( ( ( ((

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( ( ( (( ( ( ( (

(( ( ( ( (

( ( ( ( (( ( ( (

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

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( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

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( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

Airborne(Resonon(Pika(II(Sensor(0(Results(

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

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((

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Gilerson(27band(((((((Curve(Fi[ng(((((((((((((((((Mishra(PC((((((((((((((((((CI(

( ( ((

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August(17,(2015( August((24,(2015(

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September(28,(2015(Chlorophyll(a((((((((((Chlorophyll(a((((((Phycocyanin(

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Gilerson(20band(Chlorophyll((μg/L)(Chl(=(61.324*Rrs(708)/Rrs(665)(–(37.94(

(Moore(Curve(FiUng(Chlorophyll((μg/L)(X(=(Rrs(681)2/(Rrs(664)(*(Rrs(709))(Chl(=(10(71.1280*log10(x)^272.7796*log10(x)+1.0422(

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( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

( ( ( ( ( ( (

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

(

( ( ( ( ((

(( ( (

(

(

(

(

(

(

(

(

( ( ( ( ( ( ( ((

((

((

( ( (

( ( ((

(

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

(((

(

( ( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

( (

( ( ( (( ( (

( ( (

( ( ( ( (

( ( (

(

(

(

(

(

(

(

( ( ( ( ( ( ( ( (

( ((

( ( ( ( (

( ( (

(

(

(

(

(

(

(

(

(

( ( ( ( ( ( (

((

(

( ( ( (

( ( ( ( ((

( ( ( ( (

( ( (

(

(

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

(

( ( (

( ( (

( ( ( (

( (( (

( ( ( ( (

Cloud(detec7on(&(MODTRAN(Atmospheric(Correc7on((NOAA(ESRL)(

•  L(550)(<(4000(uflick(•  Morphological(opening(

with(5x5(pixel(kernel.(

•  MODTRAN(CLEAR(SKY(•  τ(550)(=(0.2(•  Laerosol(calculated(using(black(surface(

(looking(down(from(aircrag)(•  E(calculated(2015(looking(up(from(

surface(•  Lsky(calculated(as(0.2*radiance(

calculated(looking(up(from(surface(

(( (( (

(( (

(( ((( (

((

( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

( ( (( ( ( ( ( (

( ( ((( ( ( ( (

( ( (

( ( ( ( ((

(( ( (

( ( ( (( ( ( ( (

(( ( ( ( (

( ( ( ( (( ( ( (

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

(

( (  ( ( ( ( (

( ( ( ( (( ( (

(  ( ( (

((  ( ( ( ( ( (

( ( ( (( ((

((

( ((

(( ((

( (((((((((( ( (((((( ( ( (((((((((( ( (((((( (

( (

((

(

( (

( (((

( (

(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

measured aerosol skyL L LRrs

E− −

=

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ((

( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

( ( ( ( ( ( (

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

(

Hand0held(Satlan7c(Hypergun(Sensor(0(Results(

(

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

(((

((

( ( (

( ( ((

(

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

(((

(

( ( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

( (

( ( ( (( ( (

( ( (

y(=(0.1207x(7(4.6987(

R²(=(0.24216(

0(

20(

40(

60(

80(

100(

120(

0( 100( 200( 300( 400( 500( 600( 700( 800(

Mishra(Phycocyanin((

y(=(0.3828x(+(25.638(

R²(=(0.59568(

0(

50(

100(

150(

200(

250(

300(

350(

400(

0( 100( 200( 300( 400( 500( 600(

field(sa

mple(CH

L(

Moore(Curve(FiUng(CHL(

(Moore(Curve(FiUng(CHL((

y(=(0.6502x(+(20.531(

R²(=(0.71442(

0(

50(

100(

150(

200(

250(

300(

350(

400(

7100( 0( 100( 200( 300( 400( 500( 600(

field(sa

mple(CH

L(

Gilerson(20band(CHL(

GILERSON(20Band(CHL(

( ( ( (

( (( (

( ( ( ( (

( ( ( ( ( ( ( (

  ( ( ( (  ( (

( ( ( (

  ( ( (  ( ( (  ( ( ( ( (

( ( ( (  ( ( ( ( ( (

(  ( ( ( (

( ( ( ( (

(( (( (

(( (

(( ((( (

((

( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

Remote(sensing(reflectance(Scum(spectra(from(August(31,(2015(

Remote(sensing(reflectance(Non7scum((and(non7microcysLs(spectra(from(

October(14,(2015(

( ( ( ( ((

(( ( (

( ( ( (( ( ( ( (

(( ( ( ( (

( ( ( ( (( ( ( (

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

(

( (  ( ( ( ( (

( ( ( ( (( ( (

(  ( ( (

((  ( ( ( ( ( (

( ( ( (( ((

((

( ((

(( ((

( (((((((((( ( (((((( ( ( (((((((((( ( (((((( (

Mishra(Phycocyanin(

Field(sample(ph

ycocya

nin(

( (

( (((

( (

(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ((

( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

( ( ( ( ( ( (

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

(

( ( ( ( ((

(

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

(((

((

( ( (

( ( ((

(

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

(((

(

( ( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

( (

( ( ( (( ( (

( ( (

( ( ( ( (

( ( (

(

(

(

(

(

(

(

( ( ( ( ( ( ( ( (

( ((

( ( ( ( (

( ( (

(

(

(

(

(

(

(

(

(

( ( ( ( ( ( (

((

(

( ( ( (

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