chlorophyll a and phycocyanin from hyperspectral airborne...

15
Spectral Range 400/900 nm Spatial Resolution 2.1 nm (depending on altitude) Number of channels 240 Field of View 16° Chlorophyll a and Phycocyanin from Hyperspectral Airborne and Hand-held Sensors on Lake Erie 1 Andrea Vander Woude, 1 Dack Stuart, 2 Steve Ruberg, 1 Thomas Johengen, 3 Brandi McCarty, 3 Jim Churnside, 1 Danna Palladino, 1 Ashley Burtner 1 University of Michigan CooperaLve InsLtute of Limnology and Ecosystems Research, 2 NOAA Great Lakes Environmental Research Laboratory, 3 NOAA Earth System Research Laboratory Airborne Resonon Pika II Sensor - Results Research Ques7on: Are chlorophyll a and phycocyanin in blue-green algae detectable from hyperspectral sensors? Why?: To warn water intake managers in the Western basin of Lake Erie when any bloom is present near their intake References Hand-held Satlan7c Hypergun Sensor - Results Algorithms R² = 0.74593 0.000000 10.000000 20.000000 30.000000 40.000000 50.000000 60.000000 70.000000 0 20 40 60 80 100 120 140 Resonon Chl. 2 band (ug/L) Laboratory Chlorophyll (ug/L) R² = 0.61205 0.000000 5.000000 10.000000 15.000000 20.000000 25.000000 30.000000 35.000000 40.000000 0 20 40 60 80 100 120 140 Resonon Chl. Curve FiKng (ug/L) Laboratory Chlorophyll (ug/L) Gilerson 2-band Curve Fi^ng Mishra PC CI R² = 0.00581 0.0000000 50.0000000 100.0000000 150.0000000 200.0000000 250.0000000 300.0000000 350.0000000 0 20 40 60 80 100 120 140 MIshra PC Laboratory PC August 17, 2015 August 24, 2015 y = 0.1207x - 4.6987 R² = 0.24216 0 20 40 60 80 100 120 0 100 200 300 400 500 600 700 800 Mishra Phycocyanin y = 0.3828x + 25.638 R² = 0.59568 0 50 100 150 200 250 300 350 400 0 100 200 300 400 500 600 field sample CHL Moore Curve FiKng CHL Moore Curve FiKng CHL y = 0.6502x + 20.531 R² = 0.71442 0 50 100 150 200 250 300 350 400 -100 0 100 200 300 400 500 600 field sample CHL Gilerson 2-band CHL GILERSON 2-Band CHL Airborne Resonon Pika II Satlan7c Hypergun Hand-held Sensor NASA Coincident Flyovers & Intercalibra7on Cloud detec7on & MODTRAN Atmospheric Correc7on (NOAA ESRL) L(550) < 4000 uflick Morphological opening with 5x5 pixel kernel. MODTRAN CLEAR SKY τ(550) = 0.2 L aerosol calculated using black surface (looking down from aircrag) E calculated 2015 looking up from surface L sky calculated as 0.2*radiance calculated looking up from surface Hyperspectral ocean color sensor that measures water- leaving spectral radiance and sky downwelling irradiance. September 28, 2015 Chlorophyll a Chlorophyll a Phycocyanin CI 2015 coincident flyovers over Lake Erie and inter- calibraLon of sensors Remote sensing reflectance Scum spectra from August 31, 2015 Remote sensing reflectance Non-scum and non-microcysLs spectra from October 14, 2015 Mishra Phycocyanin (PC - μg/L) Ψ=R rs (560)/R rs (665) PC3=(R rs (620) -1 -Ψ*R rs (665) -1 )*R rs (778) PC = 480.92*PC 3 +123.23 Gilerson 2-band Chlorophyll (μg/L) Chl = 61.324*R rs (708)/R rs (665) – 37.94 Moore Curve FiKng Chlorophyll (μg/L) X=R rs (681) 2 /(R rs (664) * R rs (709)) Chl = 10 -1.1280*log10(x)^2-2.7796*log10(x)+1.0422 A.A. Gilerson, Gitelson, A.A., Zhou, J., Gurlin, D., Moses, W., Ioannou, I., and S.A. Ahmed. (2010) Algorithms for remote esLmaLon of chlorophyll-a in coastal and inland waters using red and near infrared bands. OpLcs Express 18(23): 24,109-24,125. S. Mishra and D.R. Mishra. (2014) A novel remote sensing algorithm to quanLfy phycocyanin in cyanobacterial algal blooms. Environmental Research Leqers. Proc. Of SPIE Vol. 9263. Future Work FuncLonal group maps of Lake Erie with a combinaLon of absorpLon and backscaqer spectra 4 different phytoplankton groups. End product will be maps of funcLonal groups for each over flight. hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/ Chlorophyta/e-source/Pediastrum%20duplex.html hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/ MicrocysLs/sp_03.html Cyanobacteria - Microcys7s Green Algae/ Chlorophyta - Pediastrum Chlorophyll a Chlorophyll a Phycocyanin CI Chlorophyll a Chlorophyll a Phycocyanin CI Mishra Phycocyanin Field sample phycocyanin hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/ Diatoms/Cards/Stephanodiscus.html hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/ Rhodomonas_Image_page.htm Cryptophy7es – Rhodomonas Bacillariophytes – Diatoms/ Dinoflagellates Gilerson 2-band Curve Fi^ng Mishra PC CI Gilerson 2-band Curve Fi^ng Mishra PC CI measured aerosol sky L L L Rrs E = Integra7ve HAB Approach Water Intake Loca7ons

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Page 1: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

Page 2: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Integra7veHAB

Approach

WaterIntakeLoca7ons

Page 3: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

( " ( ( ( (( ( ( ( ( (

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x

Page 4: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ((

( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

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Satlan7c( Hypergun(Hand0held( Sensor(

( ( ( ( (

( ( ( ( ( ( ( (

  ( ( ( (  ( (

( ( ( (

  ( ( (  ( ( (  ( ( ( ( (

( ( ( (  ( ( ( ( ( (

(  ( ( ( (

( ( ( ( (

Hyperspectral(ocean(color(sensor(that(measures(water7leaving(spectral(radiance((and((sky(downwelling(irradiance.((

( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

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Page 5: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

Page 6: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

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Airborne(Resonon(Pika(II(Sensor(0(Results(

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Gilerson(27band(((((((Curve(Fi[ng(((((((((((((((((Mishra(PC((((((((((((((((((CI(

( ( ((

(

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August(17,(2015( August((24,(2015(

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  ( ( (  ( ( (  ( ( ( ( (

( ( ( (  ( ( ( ( ( (

(  ( ( ( (

( ( ( ( (

(( (( (

(( (

(( ((( (

((

September(28,(2015(Chlorophyll(a((((((((((Chlorophyll(a((((((Phycocyanin(

( ( (( ( ( (

( ( ((

( ( (( ( ( ( ( (

( ( ((( ( ( ( (

( ( (

( ( ( ( ((

(( ( (

( ( ( (( ( ( ( (

(( ( ( ( (

( ( ( ( (( ( ( (

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

(

( (  ( ( ( ( (

( ( ( ( (( ( (

(  ( ( (

((  ( ( ( ( ( (

( ( ( (( ((

((

( ((

(( ((

Chlorophyll(a((((((((((Chlorophyll(a((((((Phycocyanin( Chlorophyll(a((((((((((Chlorophyll(a((((((Phycocyanin(

( (

((

(

( (

( (((

( (

(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

Gilerson(27band(((((((Curve(Fi[ng(((((((((((((((((Mishra(PC((((((((((((((((((CI( Gilerson(27band(((((((Curve(Fi[ng(((((((((((((((((Mishra(PC((((((((((((((((((CI(

CI CI CI

Page 7: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ((

( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

( ( ( ( ( ( (

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

(

( ( ( ( ((

(

R²(=(0.74593(0.000000(

10.000000(

20.000000(

30.000000(

40.000000(

50.000000(

60.000000(

70.000000(

0( 20( 40( 60( 80( 100( 120( 140(

Resono

n(Ch

l.(2(ba

nd((u

g/L)(

Laboratory(Chlorophyll((ug/L)(

R²(=(0.61205(0.000000(

5.000000(

10.000000(

15.000000(

20.000000(

25.000000(

30.000000(

35.000000(

40.000000(

0( 20( 40( 60( 80( 100( 120( 140(

Resono

n(Ch

l.(Cu

rve(FiUng

(((ug

/L)(

Laboratory(Chlorophyll((ug/L)(

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

R²(=(0.00581(0.0000000(

50.0000000(

100.0000000(

150.0000000(

200.0000000(

250.0000000(

300.0000000(

350.0000000(

0( 20( 40( 60( 80( 100( 120( 140(

MIshra(PC

(

Laboratory(PC(

( ( ( (( ( (

( ( (

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( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

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( ( (

Mishra(Phycocyanin((PC(0(μg/L)(Ψ=Rrs(560)/Rrs(665)(PC3=(Rrs(620)717Ψ*Rrs(665)71)*Rrs(778)(PC(=(480.92*PC3+123.23(

Gilerson(20band(Chlorophyll((μg/L)(Chl(=(61.324*Rrs(708)/Rrs(665)(–(37.94(

(Moore(Curve(FiUng(Chlorophyll((μg/L)(X(=(Rrs(681)2/(Rrs(664)(*(Rrs(709))(Chl(=(10(71.1280*log10(x)^272.7796*log10(x)+1.0422(

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

(

( (  ( ( ( ( (

( ( ( ( (( ( (

(  ( ( (

((  ( ( ( ( ( (

( ( ( (( ((

((

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( (((((((((( ( (((((( ( ( (((((((((( ( (((((( (

( (

((

(

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(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

Page 8: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ((

( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

( ( ( ( ( ( (

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

(

( ( ( ( ((

(( ( (

(

(

(

(

(

(

(

(

( ( ( ( ( ( ( ((

((

((

( ( (

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(

(

(

(

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((

(((

(

( ( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

( ( ((

(

(

(

(

(

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((

( (

( ( ( (( ( (

( ( (

( ( ( ( (

( ( (

(

(

(

(

(

(

(

( ( ( ( ( ( ( ( (

( ((

( ( ( ( (

( ( (

(

(

(

(

(

(

(

(

(

( ( ( ( ( ( (

((

(

( ( ( (

( ( ( ( ((

( ( ( ( (

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(

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(

(

(

( ( ( ( ( ( ( (

((

(

( ( (

( ( (

( ( ( (

( (( (

( ( ( ( (

Cloud(detec7on(&(MODTRAN(Atmospheric(Correc7on((NOAA(ESRL)(

•  L(550)(<(4000(uflick(•  Morphological(opening(

with(5x5(pixel(kernel.(

•  MODTRAN(CLEAR(SKY(•  τ(550)(=(0.2(•  Laerosol(calculated(using(black(surface(

(looking(down(from(aircrag)(•  E(calculated(2015(looking(up(from(

surface(•  Lsky(calculated(as(0.2*radiance(

calculated(looking(up(from(surface(

(( (( (

(( (

(( ((( (

((

( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

( ( (( ( ( ( ( (

( ( ((( ( ( ( (

( ( (

( ( ( ( ((

(( ( (

( ( ( (( ( ( ( (

(( ( ( ( (

( ( ( ( (( ( ( (

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

(

( (  ( ( ( ( (

( ( ( ( (( ( (

(  ( ( (

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( ( ( (( ((

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( (

((

(

( (

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( (

(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

measured aerosol skyL L LRrs

E− −

=

Page 9: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

Page 10: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

)

( " ( ( ( (( ( ( ( ( (

( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ((

( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( (

( ( ( ( ( ( (

( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (

(

Hand0held(Satlan7c(Hypergun(Sensor(0(Results(

(

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

(((

((

( ( (

( ( ((

(

(

(

(

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(

(

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((

(((

(

( ( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

( ( ((

(

(

(

(

(

(

(

( ( ( ( ( ( ( (

((

( (

( ( ( (( ( (

( ( (

y(=(0.1207x(7(4.6987(

R²(=(0.24216(

0(

20(

40(

60(

80(

100(

120(

0( 100( 200( 300( 400( 500( 600( 700( 800(

Mishra(Phycocyanin((

y(=(0.3828x(+(25.638(

R²(=(0.59568(

0(

50(

100(

150(

200(

250(

300(

350(

400(

0( 100( 200( 300( 400( 500( 600(

field(sa

mple(CH

L(

Moore(Curve(FiUng(CHL(

(Moore(Curve(FiUng(CHL((

y(=(0.6502x(+(20.531(

R²(=(0.71442(

0(

50(

100(

150(

200(

250(

300(

350(

400(

7100( 0( 100( 200( 300( 400( 500( 600(

field(sa

mple(CH

L(

Gilerson(20band(CHL(

GILERSON(20Band(CHL(

( ( ( (

( (( (

( ( ( ( (

( ( ( ( ( ( ( (

  ( ( ( (  ( (

( ( ( (

  ( ( (  ( ( (  ( ( ( ( (

( ( ( (  ( ( ( ( ( (

(  ( ( ( (

( ( ( ( (

(( (( (

(( (

(( ((( (

((

( ( (( (((((((((( ( (((((( (

( ( (( ( ( (

( ( ((

Remote(sensing(reflectance(Scum(spectra(from(August(31,(2015(

Remote(sensing(reflectance(Non7scum((and(non7microcysLs(spectra(from(

October(14,(2015(

( ( ( ( ((

(( ( (

( ( ( (( ( ( ( (

(( ( ( ( (

( ( ( ( (( ( ( (

(

( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ((( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( (( ( ( ( ( ( ( ( ((

(

( (  ( ( ( ( (

( ( ( ( (( ( (

(  ( ( (

((  ( ( ( ( ( (

( ( ( (( ((

((

( ((

(( ((

( (((((((((( ( (((((( ( ( (((((((((( ( (((((( (

Mishra(Phycocyanin(

Field(sample(ph

ycocya

nin(

( (

( (((

( (

(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

Page 11: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

Page 12: Chlorophyll a and Phycocyanin from Hyperspectral Airborne ......R²(=(0.74593(0.000000(10.000000(20.000000(30.000000(40.000000(50.000000(60.000000(70.000000(0( 20( 40( 60( 80( 100(

Spectral)Range) 400/900)nm)Spatial)Resolution) 2.1)nm)(depending)on)altitude))Number)of)channels) 240)

Field)of)View) 16°))

ChlorophyllaandPhycocyaninfromHyperspectralAirborneandHand-heldSensorsonLakeErie

1AndreaVanderWoude,1DackStuart,2SteveRuberg,1ThomasJohengen,3BrandiMcCarty,3JimChurnside,1DannaPalladino,1AshleyBurtner

1UniversityofMichiganCooperaLveInsLtuteofLimnologyandEcosystemsResearch,2NOAAGreatLakesEnvironmentalResearchLaboratory,3NOAAEarthSystemResearchLaboratory

AirborneResononPikaIISensor-Results

ResearchQues7on:Arechlorophyllaandphycocyanininblue-greenalgaedetectablefromhyperspectralsensors?Why?:TowarnwaterintakemanagersintheWesternbasinofLakeEriewhenanybloomispresentneartheirintake.

References

Hand-heldSatlan7cHypergunSensor-Results

Algo

rithm

s

R²=0.745930.000000

10.000000

20.000000

30.000000

40.000000

50.000000

60.000000

70.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.2ba

nd(u

g/L)

LaboratoryChlorophyll(ug/L)

R²=0.612050.000000

5.000000

10.000000

15.000000

20.000000

25.000000

30.000000

35.000000

40.000000

0 20 40 60 80 100 120 140

Resono

nCh

l.Cu

rveFiKng

(ug

/L)

LaboratoryChlorophyll(ug/L)

Gilerson2-bandCurveFi^ngMishraPCCI

R²=0.005810.0000000

50.0000000

100.0000000

150.0000000

200.0000000

250.0000000

300.0000000

350.0000000

0 20 40 60 80 100 120 140MIshraPC

LaboratoryPC

August17,2015 August24,2015

y=0.1207x-4.6987

R²=0.24216

0

20

40

60

80

100

120

0 100 200 300 400 500 600 700 800

MishraPhycocyanin

y=0.3828x+25.638

R²=0.59568

0

50

100

150

200

250

300

350

400

0 100 200 300 400 500 600

fieldsa

mpleCH

L

MooreCurveFiKngCHL

MooreCurveFiKngCHL

y=0.6502x+20.531

R²=0.71442

0

50

100

150

200

250

300

350

400

-100 0 100 200 300 400 500 600

fieldsa

mpleCH

L

Gilerson2-bandCHL

GILERSON2-BandCHL

AirborneResononPikaII

Satlan7c HypergunHand-held Sensor

NASACoincidentFlyovers&Intercalibra7on

Clouddetec7on&MODTRANAtmosphericCorrec7on(NOAAESRL)

•  L(550)<4000uflick•  Morphologicalopening

with5x5pixelkernel.

•  MODTRANCLEARSKY•  τ(550)=0.2•  Laerosolcalculatedusingblacksurface

(lookingdownfromaircrag)•  Ecalculated2015lookingupfrom

surface•  Lskycalculatedas0.2*radiance

calculatedlookingupfromsurface

Hyperspectraloceancolorsensorthatmeasureswater-leavingspectralradianceandskydownwellingirradiance.

September28,2015ChlorophyllaChlorophyllaPhycocyaninCI

2015coincidentflyoversoverLakeErieandinter-calibraLonofsensors

RemotesensingreflectanceScumspectrafromAugust31,2015

RemotesensingreflectanceNon-scumandnon-microcysLsspectrafrom

October14,2015

MishraPhycocyanin(PC-μg/L)Ψ=Rrs(560)/Rrs(665)PC3=(Rrs(620)-1-Ψ*Rrs(665)-1)*Rrs(778)PC=480.92*PC3+123.23

Gilerson2-bandChlorophyll(μg/L)Chl=61.324*Rrs(708)/Rrs(665)–37.94

MooreCurveFiKngChlorophyll(μg/L)X=Rrs(681)2/(Rrs(664)*Rrs(709))Chl=10-1.1280*log10(x)^2-2.7796*log10(x)+1.0422

A.A.Gilerson,Gitelson,A.A.,Zhou,J.,Gurlin,D.,Moses,W.,Ioannou,I.,andS.A.Ahmed.(2010)AlgorithmsforremoteesLmaLonofchlorophyll-aincoastalandinlandwatersusingredandnearinfraredbands.OpLcsExpress18(23):24,109-24,125.S.MishraandD.R.Mishra.(2014)AnovelremotesensingalgorithmtoquanLfyphycocyaninincyanobacterialalgalblooms.EnvironmentalResearchLeqers.Proc.OfSPIEVol.9263.

FutureWork•  FuncLonalgroupmapsofLake

EriewithacombinaLonofabsorpLonandbackscaqerspectra

•  4differentphytoplanktongroups.

•  EndproductwillbemapsoffuncLonalgroupsforeachoverflight.

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e-algae/Chlorophyta/e-source/Pediastrum%20duplex.html

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html

Cyanobacteria-Microcys7s

GreenAlgae/Chlorophyta-Pediastrum

ChlorophyllaChlorophyllaPhycocyaninCI ChlorophyllaChlorophyllaPhycocyaninCI

MishraPhycocyanin

Fieldsampleph

ycocya

nin

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm

Cryptophy7es–Rhodomonas

Bacillariophytes–Diatoms/Dinoflagellates

Gilerson2-bandCurveFi^ngMishraPCCI Gilerson2-bandCurveFi^ngMishraPCCI

measured aerosol skyL L LRrs

E− −

=

Integra7veHAB

Approach

WaterIntakeLoca7ons

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) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

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) ) ) )) ) ) ) ) ) )) ) ) )) ) ) )

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(

Future(Work(•  FuncLonal(group(maps(of(Lake(

Erie(with(a(combinaLon(of(absorpLon(and(backscaqer(spectra(

•  4(different(phytoplankton(groups.((

•  End(product(will(be(maps(of(funcLonal(groups(for(each(over(flight.((

hqp://www.plingfactory.de/Science/Atlas/Kennkarten%20Algen/01_e7algae/Chlorophyta/e7source/Pediastrum%20duplex.html(

hqp://proLst.i.hosei.ac.jp/pdb/images/Prokaryotes/Chroococcaceae/MicrocysLs/sp_03.html(

Cyanobacteria(0(Microcys7s(

Green(Algae/Chlorophyta(0(Pediastrum(

( (((((((((( ( (((((( ( ( (((((((((( ( (((((( (

( ((

((

hqp://www.glerl.noaa.gov/seagrant/GLWL/Algae/Diatoms/Cards/Stephanodiscus.html(

hqp://cv.unh.edu/phycokey/Choices/Cryptophyceae/RHODOMONAS/Rhodomonas_Image_page.htm(

Cryptophy7es(–((Rhodomonas(

Bacillariophytes(–(Diatoms/Dinoflagellates(

( ( (

( (((((( ( (((((((((((( ( ((((((((

( (((((((((( ( (((((( (

( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( ( ( ((((((( ( ((((((((((((((((( ( (((((((((((((((((( (

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QuesLons?