capillary electrophoresis by sunil budke

CAPILLARY ELECTROPHORESIS

BASIC CONCEPTS & HARDWARE

by Sunil Budke

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Source vial

Destination Vial

Capillary tube

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Electropherogram from CE

CAPILLARY Made of fused silica Polyamide coating for protection Pyrex or Teflon capillaries are available Effective length 10 - 100 cm Internal diameter (ID) 20 -100 micro meter C8 and C18 coated capillaries are

available

SAMPLE INTRODUCTION

Electrokinetic Injection5kv for 5 – 10 sec

Gravimetric (Syphonic)Sample vial held at higher level

Differential pressurePressure applied on sample vial or vacuum applied on

Destination vial

DETECTORS Conductivity Detectors

UV Detectors

Fluorescence Detector

Electrochemical Detector

Mass Spectrometer

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DISCUSSION

References Capillary Electrophoresis

-by Dale R. Baker

Capillary Electrophoresis for Food Analysis – Method Development

-by Richard A. Frazier; Jennifer M. Ames and Harry E. Nursten

Principles and Practice of Bioanalysis -by Richard F. Venn

McGraw Hill - Year book of Science and Technology – 2002-Capillary electrophoresis

Thank You