bioo scientific - improving ngs library prep automation on the sciclone ngs workstation

IMPROVING NGS LIBRARYPREP AUTOMATION

on the

SCICLONE NGS WORKSTATION

Typical NGS workflows offer challenges with respect to throughput,

cost & uniformity

Use of automated liquid handling workstations can provide a cost-

effective solution to many ofthese workflow challenges

Rapid & robust workflows which are easy to deploy are critical for success

How is Bioo Scientific improving library prep automation on the Sciclone?

EASY DEPLOYMENT

Bioo Scientific has developed & validated a number of automated RNA-Seq &

DNA-Seq Illumina-compatible library prep programs for use on the Sciclone

NGS and NGSx Workstations

RAPID & ROBUST WORKFLOWS

The NEXTflex RNA-Seq and DNA-Seq Kits automated on the Sciclone all incorporate

fast, user-friendly protocols

INCREASED MULTIPLEXING CAPABILITIES

Up to 384 unique, single index barcodesare available for deep multiplexing of

RNA-Seq & DNA-Seq libraries

REDUCED RNA-SEQ BIAS

Bias-reducing Molecular Indexes allow for more accurate resolution of duplicates

introduced during PCR amplification step of stranded RNA-Seq library construction on

the Sciclone NGS or NGSx

Absolute Quantitationfor RNA-Seq

Molecular Indexes are included in the NEXTflex™ Rapid Directional qRNA-Seq™ Kit

®

DOWNLOAD MANUAL VIEW SLIDESHARE

IMPROVED LIGATION EFFICIENCY

The NEXTflex DNA-Seq & RNA-Seq library prep kits automated on the Sciclone all incorporate Bioo Scientific’s Enhanced Adapter Ligation Efficiency Technology

Which increases library diversity & complexity, thus allowing for use of

degraded or smaller amounts of input DNA

OPTIMIZED FOR AUTOMATION

The NEXTflex DNA-Seq & RNA-Seq library prep kits include master-mixes

optimized for automation

Download the Sciclone NGS and NGSx Workstation Automation Guides:

®®®®

NEXTflex™ Rapid DirectionalqRNA-Seq™ Kit Automation Guide

NEXTflex™ Rapid DirectionalRNA-Seq Kit Automation Guide

NEXTflex™ Rapid RNA-SeqKit Automation Guide

NEXTflex™ Rapid DNA-SeqKit Automation Guide

DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL

Or contact one of our Automation Specialists today to improve your automated NGS library

prep using the Sciclone NGS Workstation

If you have any questions email us atnextgen@biooscientific.com

or visit our website atBiooScientific.com/Sciclone