bioo scientific - improving ngs library prep automation on the sciclone ngs workstation
TRANSCRIPT
IMPROVING NGS LIBRARYPREP AUTOMATION
on the
SCICLONE NGS WORKSTATION
Typical NGS workflows offer challenges with respect to throughput,
cost & uniformity
Use of automated liquid handling workstations can provide a cost-
effective solution to many ofthese workflow challenges
Rapid & robust workflows which are easy to deploy are critical for success
How is Bioo Scientific improving library prep automation on the Sciclone?
EASY DEPLOYMENT
Bioo Scientific has developed & validated a number of automated RNA-Seq &
DNA-Seq Illumina-compatible library prep programs for use on the Sciclone
NGS and NGSx Workstations
RAPID & ROBUST WORKFLOWS
The NEXTflex RNA-Seq and DNA-Seq Kits automated on the Sciclone all incorporate
fast, user-friendly protocols
INCREASED MULTIPLEXING CAPABILITIES
Up to 384 unique, single index barcodesare available for deep multiplexing of
RNA-Seq & DNA-Seq libraries
REDUCED RNA-SEQ BIAS
Bias-reducing Molecular Indexes allow for more accurate resolution of duplicates
introduced during PCR amplification step of stranded RNA-Seq library construction on
the Sciclone NGS or NGSx
Absolute Quantitationfor RNA-Seq
Molecular Indexes are included in the NEXTflex™ Rapid Directional qRNA-Seq™ Kit
®
DOWNLOAD MANUAL VIEW SLIDESHARE
IMPROVED LIGATION EFFICIENCY
The NEXTflex DNA-Seq & RNA-Seq library prep kits automated on the Sciclone all incorporate Bioo Scientific’s Enhanced Adapter Ligation Efficiency Technology
Which increases library diversity & complexity, thus allowing for use of
degraded or smaller amounts of input DNA
OPTIMIZED FOR AUTOMATION
The NEXTflex DNA-Seq & RNA-Seq library prep kits include master-mixes
optimized for automation
Download the Sciclone NGS and NGSx Workstation Automation Guides:
®®®®
NEXTflex™ Rapid DirectionalqRNA-Seq™ Kit Automation Guide
NEXTflex™ Rapid DirectionalRNA-Seq Kit Automation Guide
NEXTflex™ Rapid RNA-SeqKit Automation Guide
NEXTflex™ Rapid DNA-SeqKit Automation Guide
DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL
Or contact one of our Automation Specialists today to improve your automated NGS library
prep using the Sciclone NGS Workstation
If you have any questions email us [email protected]
or visit our website atBiooScientific.com/Sciclone