02 laurent fischer

CIRCOVAC piglet efficacy

Laurent Fischer, DVM, PhDDirector, Senior Technical Leader R&D, Project

and Portfolio Management - MERIAL

CIRCOVAC piglet efficacy

• CIRCOVAC: characteristics• Registration dossier

– Experimental– Field data

CIRCOVAC: characteristics

• Inactivated, PCV2a strain

• Oily adjuvant (TS6)

• Sows and gilts: 2 ml / dose

• Piglets: 0.5 ml / dose

• From 3 weeks of age

• Very good safety profile with minimal local reactions

Piglet indications

Active immunisation

Clinical signs

wasting

mortality

weight loss

Virus load

Faecal excretion

Lesions and virus load in lymphoid tissues

0

0,5

1

1,5

2

2,5

0 7 14 21 28 35 42

Days after vaccination

Tit

re (

log

10 P

D50

)

Control

CIRCOVAC

Active immunisation

Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 439Dossier A.M.M. CIRCOVAC. N°08.609Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 439Dossier A.M.M. CIRCOVAC. N°08.609

CIRCOVAC piglets vaccination induces seroneutralizing antibodies: quick onset and high titres

D0 = Vaccination

0

1

2

3

4

5

6

J -1 J21 J49 J77 J98 J119 J140

Days after vaccination

* **

2

2,5

3

3,5

4

4,5

5

J -1 J7 J14 J21 J28

Days after challenge

6

Virus load decreased

Significant less pigs positive for viremia and the mean viral load was significantly decreased in the CIRCOVAC vaccinated group

Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 437Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 437

D0 = Challenge D0 = Vaccination

Expérimental conditions Field

* p<0,0001

Faecal excretion reduced

Dossier A.M.M. CIRCOVAC. N° 08.0032.PDossier A.M.M. CIRCOVAC. N° 08.0032.P

01234567

D-1 D21 D49 D77 D98 D119 D140

Days after vaccination

D0 = Vaccination

*

*

*Student t test p<0,0001

Lymphoid lesions reduced

15

00

Dossier A.M.M. CIRCOVAC. N° 08.0610.RDossier A.M.M. CIRCOVAC. N° 08.0610.R

*Fisher test p<0,001*Fisher test p<0,001

No lesion80 % lesions

Control

CIRCOVAC*

Nulle Modéré Forte

Témoins négatifs

Nulle Modéré Forte

Virus load reduced

Dossier A.M.M. CIRCOVAC. N° 08.0610.RDossier A.M.M. CIRCOVAC. N° 08.0610.R

Virus load (IHC) of mesenteric lymph nodes

Control Photo: Pr Sarli

*Fisher test p<0,001*Fisher test p<0,001

Lymphoid lesions reduced

6,59

2

3,79

5,35

3,2

8,39

01234

56789

I nguinal Mediastinal Mesenterique

Control CIRCOVAC

*Student t test p<0,05*Student t test p<0,05

Dossier A.M.M. CIRCOVAC. N° 08.0338.RDossier A.M.M. CIRCOVAC. N° 08.0338.R

* *

Weight (g) of lymph nodes

* *

Lymphoid lesions reduced

Control

CIRCOVAC piglets vaccination is efficient

• Benefits– Health status improved

• PCV2 viremia decreased• Faecal shedding decreased• Lymphoid lesions controlled

– Mortality and wasting decreased from weaning up to slaughter

– Growth performance improved from weaning to slaughter

Thank you!