02 laurent fischer
TRANSCRIPT
CIRCOVAC piglet efficacy
Laurent Fischer, DVM, PhDDirector, Senior Technical Leader R&D, Project
and Portfolio Management - MERIAL
CIRCOVAC piglet efficacy
• CIRCOVAC: characteristics• Registration dossier
– Experimental– Field data
CIRCOVAC: characteristics
• Inactivated, PCV2a strain
• Oily adjuvant (TS6)
• Sows and gilts: 2 ml / dose
• Piglets: 0.5 ml / dose
• From 3 weeks of age
• Very good safety profile with minimal local reactions
Piglet indications
Active immunisation
Clinical signs
wasting
mortality
weight loss
Virus load
Faecal excretion
Lesions and virus load in lymphoid tissues
0
0,5
1
1,5
2
2,5
0 7 14 21 28 35 42
Days after vaccination
Tit
re (
log
10 P
D50
)
Control
CIRCOVAC
Active immunisation
Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 439Dossier A.M.M. CIRCOVAC. N°08.609Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 439Dossier A.M.M. CIRCOVAC. N°08.609
CIRCOVAC piglets vaccination induces seroneutralizing antibodies: quick onset and high titres
D0 = Vaccination
0
1
2
3
4
5
6
J -1 J21 J49 J77 J98 J119 J140
Days after vaccination
* **
2
2,5
3
3,5
4
4,5
5
J -1 J7 J14 J21 J28
Days after challenge
6
Virus load decreased
Significant less pigs positive for viremia and the mean viral load was significantly decreased in the CIRCOVAC vaccinated group
Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 437Chevalier et al, Proceedings of the 21st IPVS Congress, Vancouver, Canada, 2010, p 437
D0 = Challenge D0 = Vaccination
Expérimental conditions Field
* p<0,0001
Faecal excretion reduced
Dossier A.M.M. CIRCOVAC. N° 08.0032.PDossier A.M.M. CIRCOVAC. N° 08.0032.P
01234567
D-1 D21 D49 D77 D98 D119 D140
Days after vaccination
D0 = Vaccination
*
*
*Student t test p<0,0001
Lymphoid lesions reduced
15
00
Dossier A.M.M. CIRCOVAC. N° 08.0610.RDossier A.M.M. CIRCOVAC. N° 08.0610.R
*Fisher test p<0,001*Fisher test p<0,001
No lesion80 % lesions
Control
CIRCOVAC*
Nulle Modéré Forte
Témoins négatifs
Nulle Modéré Forte
Virus load reduced
Dossier A.M.M. CIRCOVAC. N° 08.0610.RDossier A.M.M. CIRCOVAC. N° 08.0610.R
Virus load (IHC) of mesenteric lymph nodes
Control Photo: Pr Sarli
*Fisher test p<0,001*Fisher test p<0,001
Lymphoid lesions reduced
6,59
2
3,79
5,35
3,2
8,39
01234
56789
I nguinal Mediastinal Mesenterique
Control CIRCOVAC
*Student t test p<0,05*Student t test p<0,05
Dossier A.M.M. CIRCOVAC. N° 08.0338.RDossier A.M.M. CIRCOVAC. N° 08.0338.R
* *
Weight (g) of lymph nodes
* *
Lymphoid lesions reduced
Control
CIRCOVAC piglets vaccination is efficient
• Benefits– Health status improved
• PCV2 viremia decreased• Faecal shedding decreased• Lymphoid lesions controlled
– Mortality and wasting decreased from weaning up to slaughter
– Growth performance improved from weaning to slaughter
Thank you!