Announcement of Population Data

Allele frequencies for nine STR loci in a Korean

ethnic group from northeast China

YongJi Zhang a,b,*, QingSong Xu a, Hong Cui a, Yan Cui a,KiBeom Kim b, JungBin Lee b

aDepartment of Forensic Medicine, Yanbian University College of Medicine,

121 Juzi-jie, Yanji 133000, Jilin, P.R. ChinabDepartment of Forensic Medicine, College of Medicine, Seoul National University,

28 Yeongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea

Received 27 November 2004; received in revised form 14 January 2005; accepted 14 January 2005

Available online 16 February 2005

Abstract

Allele frequencies for the nine tetrameric short tandem repeats (STR) loci D3S1358, vWA, FGA, D8S1179, D21S11,

D18S51, D5S818, D13S317, D7S820 (AmpF‘STR1 Profiler PlusTM PCR Amplification kit, PE Applied Biosystems) were

determined in 109 unrelated Korean ethnic group individuals from northeast China.

# 2005 Elsevier Ireland Ltd. All rights reserved.

Keywords: STR; AmpF‘STR Profiler plus; Korean ethnic group; Northeast China

www.elsevier.com/locate/forsciint

Forensic Science International 155 (2005) 216–218

Population: Healthy unrelated Korean ethnic group

volunteers living in the northeastern part of China so called

Yan Bian area.

DNA extraction: Phenol–chloroform–isoamyl alcohol

extraction [1].

PCR: A 1.0 ng template following manufacturer’s

instructions (AmpF‘STR1 Profiler PlusTM PCR amplifica-

tion kit) [2].

Typing: PCR products were detected on 8 M urea 5%

long ranger gel (FMC BioProducts) using ABI prism (377)

automatic sequencer (PE Applied Biosystems).

Analysis of data: Applied ‘‘PowerStars’’ program (Pro-

mega home page: http://www.promega.com/geneticidtools/)

for observed and expected heterozygosity (Ho, He), PD

(power of discrimination), PIC (polymorphism information

content) and CE (chance of exclusion), p-value according to

Guo and Thompson [3].

Result: See Table 1.

* Corresponding author. Tel.: +86 433 266 0590; fax: +86 433 265 979

E-mail address: zhangyj@ybu.edu.cn (Y. Zhang).

0379-0738/$ – see front matter # 2005 Elsevier Ireland Ltd. All rights r

doi:10.1016/j.forsciint.2005.01.005

Other remarks: No significant deviation from Hardy-

Weinberg expectations ( p > 0.05) and no significant dif-

ferences were observed in the comparison with same area

(Yan Bian) and other areas China Han (except vWA for

middle China Han) [4–15]. Also we compared to with

South Korean, Japanese, American Caucasian and

American African for the allele distribution, and found

great differences in FGA and D21S11 for Korean,

D3S1358 and vWA for Japanese, D21S11, D18S51,

D8S1179, FGA and vWA for American Caucasian and

American African [16–18]. The combined PD was more

than 0.99999999995 and CE was more than 0.9998 in the

Korean ethnic group population. So according to these

statistical parameters, the combination of these nine STR

systems is a powerful tool for forensic identification and

paternity testing.

This paper follows the guidelines for publication of

population data requested by the journal [19].

5.

eserved.

Y.J. Zhang et al. / Forensic Science International 155 (2005) 216–218 217

Table 1

Allele frequencies for nine STR loci in Korean ethnic population from northeastern part (Yan Bian) of China

Allele D3S1358 VWA FGA D8S1179 D18S51 D5S818 D13S317 D7S820 Allele D21S11

N = 109 N = 109 N = 109 N = 109 N = 109 N = 109 N = 109 N = 109 N = 109

5 – – – – – – – 0.005 28 0.037

7 – – – – – 0.023 – – 28.2 0.009

8 – – – – – 0.005 0.234 0.156 29 0.229

9 – – – – – 0.115 0.147 0.055 29.2 0.005

10 – – – 0.092 0.005 0.179 0.170 0.165 30 0.394

11 – – – 0.106 0.014 0.275 0.225 0.312 30.2 0.009

12 0.009 – – 0.156 0.041 0.252 0.161 0.271 31 0.078

13 0.005 – – 0.193 0.252 0.128 0.050 0.028 31.2 0.041

14 0.041 0.236 – 0.193 0.147 0.023 0.014 0.009 32 0.028

15 0.385 0.023 – 0.206 0.151 – – – 32.2 0.115

16 0.298 0.167 – 0.050 0.115 – – – 33 0.014

17 0.197 0.269 – 0.005 0.069 – – – 33.2 0.041

18 0.064 0.222 0.005 – 0.060 – – – – –

19 – 0.065 0.046 – 0.064 – – – – –

20 – 0.019 0.073 – 0.046 – – – – –

21 – – 0.096 – 0.023 – – – – –

21.2 – – 0.005 – – – – – – –

22 – – 0.151 – 0.009 – – – – –

22.2 – – 0.005 – – – – – – –

23 – – 0.225 – 0.005 – – – – –

24 – – 0.206 – – – – – – –

25 – – 0.124 – – – – – – –

26 – – 0.050 – – – – – – –

27 – – 0.005 – – – – – – –

28 – – 0.009 – – – – – – –

Ho 0.761 0.833 0.862 0.780 0.844 0.835 0.789 0.798 – 0.716

He 0.718 0.790 0.849 0.837 0.862 0.798 0.816 0.774 – 0.767

PD 0.862 0.917 0.953 0.949 0.957 0.916 0.939 0.909 – 0.910

PIC 0.670 0.757 0.832 0.815 0.848 0.768 0.789 0.739 – 0.739

P 0.800 0.999 0.999 0.640 0.811 0.421 0.998 0.935 – 0.837

CE 0.530 0.662 0.719 0.562 0.683 0.665 0.579 0.596 – 0.453

N: number of samples, Ho: observed heterozygosity, He: expected heterozygosity, PD: power of discrimination, PIC: polymorphic information

content, p: probability values of exact tests for Hardy-Weinberg equilibrium, CE: chance of exclusion.

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