alex adams, greer beaumont, candace booker, mark farrington, nick molligan, robert power advisors:...
TRANSCRIPT
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Controlled-Rate Freezing Device
Alex Adams, Greer Beaumont, Candace Booker, Mark Farrington, Nick Molligan, Robert Power
Advisors: Melissa Eskridge and Todd Monroe
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Clientele Information Dr. Tiersch, Amy Guitreau, and the employees at the Aqua-Cultural Research Station lab Customer baseARS LabFishing farmsBiological research labs
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Project Decomposition
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Background Cryopreservation Slow programmable freezing Example:
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Problem Statement Our team is attempting to create an inexpensive, freezing device and, in doing so, achieve a cooling rate at which straws containing sperm cells can efficiently be frozen.
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Melissa’s objective:
Our objective:
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Project Goals & Measurable Objectives
Simplified system modeled after computer controlled freezing system
Complete temperature profile of the system
Utilize Arduino to control subunits: sensory,
display, and data logger
Target cooling rate: -4°C/min
Ideal temperature: (-80°C) – (-100°C)
Ideal straw transit time: 20 minutes
Straw throughput: 50/run
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Concept Generation Arduino:
Mrs. Dugas’ classesData organization
Intro to ArduinoCodeCircuit design
Research of materials and Arduino components
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Materials Temperature Profiling:
Type T Thermocouples
Liquid Nitrogen
Styrofoam box
Mesh grid galvanized steel
Arduino: Uno (2) (Microcontroller)
LCD Screen Shield
Plastic Breadboard
Jumper Wires
Counting Sensor
SD Card
Data Logger Shield
Thermocouple Shield
Barcode Scanner
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ConstraintsTime
Liquid Nitrogen Evaporation
Data Logger % Error
Arduino Compatibility
Arduino circuitry that works at low temperatures
Range of inputs
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Design Slide
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Microcontroller Design Matrices
BBB Arduino UNO
Arduino DUE
Arduino Yun
Mintduino Galileo Raspberry Pi
Analog pins 7 6 12 12 6 6 0
Digital pins 8 14 54 20 14 14 8
Memory N/A 2KB 96KB 2.5KB 2KB 512KB N/A
Adequate Research
Yes Yes No Yes Yes Yes Yes
Cost 45 30 50 70 25 85 40
Parameters Options
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MAX31855 Multiplexer
Price $82.95 $44.50
Port Count 4 8
Probe Input Current Probes Compatable
Require New Probe Purchase
Output Resolution
0.25 C 0.25 C
Voltage Built-I n Voltage translation
3.3V or 5V
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Arduino ParametersInter Integrated communication (I^2C)
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ParametersTesting Conditions:
A/C set @ 21.1°C
Room temperature ~21.3°C
Styrofoam box dimensions:
Height: 16.5 in./7 in.
Width: 15 in./11.75 in.
Galvanized steel grid:
Lies .932 inches in the box
15.5 x 15.5 in²
Liquid Nitrogen:
4 inches (~6 L)
Temperature Logger Calibration:
See Appendix A
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Arduino ChallengesCHALLENGES:
Compatible Arduino components 3 thermocouples (displayed information) Bar code scanner Counter SD reader ( LCD
Range of inputs
SOLUTIONS:
Researching materials (model selection)
Project box (protection from temp.)
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Design Tools: Structure/Function Chart
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Gantt Chart – Progress and current location
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BudgetMaterials Quantity Cost
Type T Thermocouples 15 $5-8 per thermocouple
Barcode Scanner 1 $30-40
Arduino DUE Microcontroller 1 $49.95
Arduino UNO Microcontroller 1 $29.95
SD Memory Card 1 $11.99
Counting Sensor 1 $20-30
LCD Display Screen 1 $23.99
Chicken Wire 1 $15.00
Thermocouple Shield 1 $46.50
Data Logger Shield 1 $19.95
LCD Screen Shield 1 $21.95
Counting Sensor Shield 1 $20-30
Total Cost: $294.28 - $327.28
Outside Funding
Grant
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Thank you! Acknowledgements:
Dr. Todd Monroe
Melissa Eskridge
Amy Guitreau
Dr. Tiersch and the ARS employees
Questions?
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Appendix A Liquid mercury thermometer and 15 Type Tthermocouples
Calibrated using: Room temperature water (Table 1) Boiling water Ice water
Loggers collected 5 minutes of data
Recorded data variation connects withspecified % error of the temperature logger
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Results of Temp. Profiling Numbers & graph in-the-making:
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Temperature Profiling - Systematic technique
- Mesh grid
- Notation system
- Calibrating
- Devising our technique
- Measuring
- Straw stabilization device
- 15 minutes
- 3 different settings
- DT 300 software
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Straw Method Pictures Individual Straw Method Straw Stabilization Method