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Adaptive Strategies Based Upon Tolerant Rootstocks
Development for Abiotic Stress Tolerance in Grapevines
from Northern ChileINSTITUTO DE INVESTIGACIONES AGROPECUARIAS (INIA) CRI INTIHUASI
Dr Andrés Zurita-Silva
Santiago, February 2020
Verdugo-Vásquez et al. 2019 (in prep)
Days >30ºC Growth Season (Sept 1st- May 1st)
@ Vicuña Experimental Center
Years
Mineral concentration in petioles cv. Flame Seedless on
various rootstocks @ Vicuña Experimental Center
Antonio Ibacache, Nicolás Verdugo-Vásquez & Andrés Zurita-Silva, 2019.
Rootstock: Scion combinations and nutrient uptake in grapevines.
*Each bar represents the average of 4 years plus standard error. Different letters denote significant differences (P<.05).
148 collected
accessions
(genotyped).
20 Rootstock
lines assessed
for drought @
CEx Vicuña.
5 Rootstock
selected
genotypes.
Grapevine Germoplasm Bank from Northern
Chile (GermoVidNor).
P32 P57 P65 P70 PF 140 Ru
Measurements were performed at two-week interval along both growing
seasons:
• Shoot leaf number (ShLN)
• Area (ShA)
• Trunk cross sectional area (TkCSA)
• Rootstock CSA (RsCSA),
• Stem water potential (Ψx) by pressure chamber PMS 1505D
• Stomatal conductance (gs), Net photosynthesis (Pn) by Li-Cor 6400 XTR
IRGA.
Root traits (area [RtA], volume [RtV] and length [RtL]) estimated monthly
from accession tubes in each pot by Rootscan Imager (CID BioScience),
analyzed with Rootsnap 1.3.2.25 software.
RNA Isolation and cDNA Synthesis
• 100mg Leaf tissues were sampled and RNA was extracted by 2% CTAB /
LiCl2 precipitation, purified and treated with RQ1 RNase-Free DNase.
• First strand DNA was synthesized from 2 μg of total RNA using High
capacity RNA kit for cDNA.
Gene expression analysis by qRT-PCR
• Diluted cDNA (10 ng/μL) was assessed for quantification of specific
primer pairs products using SYBR Green QPCR Master mix in AriaMx
Materials & Methods
Functional Trait Responses to Irrigation (T100) &
30%Deficit (T30) in cv Cabernet Sauvignon & Syrah
Rootstocks effects over responses to 30 % irrigation
Principal Components (PC) Season 2 for 30% DI
R32 was closely associated to ShA irrespective of season and cultivar and opposed to Pn, gs and Yx.
Higher ShA was related to increased root growth and rootstock and trunk CSA, traits which were
also closely related to R32 for both cultivars.
Franck et al., Scientia Horticulturae 262 (2020)
Cabernet Sauvignon & Syrah grafted into R32 in two
irrigation regimes for RNA-Seq studies
Franck et al., Scientia Horticulturae 262 (2020)
535 412 1214
Leaf specific DEG
Root specific DEG
633 1474 2287
Cabernet Sauvignon
Syrah
Number of Differentially Expressed Genes
(DEG) grouped by tissues
Franck et al., Scientia Horticulturae 262 (2020)
Functional trait responses to irrigation and 50% Deficit
irrigation in grafted cv Cabernet Sauvignon & Syrah
@ CExp-Vicuña
Phenology Responses to Water Deficit (50% WD) in cv
Syrah & Cabernet Sauvignon grafted into selected
Rootstocks
Variance explained by each factor and interaction (RxI) for phenological variables in each cv and season.
Significant portion of variance (p<0.05) is shown in Variance explained percentage and color intensity.
Verdugo-Vásquez et al. 2020 (in prep)
Leaf Nutrient Content at Veraison @ CExp-Vicuña
ACKNOWLEDGEMENTS
FONDECYT REGULAR 1140039
INNOVA Chile 05CR11PAT-19 Genotipos Radiculares
Dr Nicolás Franck
M Sc Antonio Ibacache
Dr Nicolás Verdugo
Dr Claudio Balbontín
MgSc Irina Díaz
MgSc Alejandra Montoya
Agr Denisse Zamorano
Agr Britt Walberg
Agr Charlotte Hardy
Agr Natalia Rivera
Agr Mario Ahumada
Dr Claudio Meneses UNAB
Dr Iván Balic ULL
Claudio Urra UNAB
Macarena Bastías UNAB
Dr Pedro León
Dr Humberto Prieto
Dr Javier Ibáñez ICVV