acquity uplc csh and xselect hplc columns · we trust you find this useful in choosing the right...

[ ACQUITY UPLC CSH and XSelect HPLC Columns ]

True Evolution in LC Column PerformanceTrue Evolution in LC Column Performance

CSH™ Technology dramatically improves virtually all facets of LC column performance in acidic, low ionic

strength mobile phases that are commonly used in the chromatographic laboratory.

PARTICLE SIZE CHEMISTRY BRAND

1.7 µm ACQUITY UPLC CSH C18

ACQUITY UPLC CSH Fluoro-Phenyl

ACQUITY UPLC CSH Phenyl-Hexyl

3.5 µm

XSelect CSH C18

XSelect CSH Fluoro-Phenyl

XSelect CSH Phenyl-Hexyl

5 µm

4

For over 50 years, Waters Corporation has enabled our customers with products and applications tailored to their needs. All applications presented in our literature include detailed methodology to demonstrate typical use procedures and enable replication in customer laboratories. We trust you find this useful in choosing the right product for your needs.

Charged Surface Hybrid (CSH) Technology

Waters has been at the forefront of materials science as applied to chromatographic media for the last 50 years. Recent stationary phase innovations include ultra-pure silica for

improved peak shape for basic compounds; optimized pore properties and bonded phase coverage for polar compound retention and aqueous mobile-phase compatibility; and most

importantly, hybrid particle technology (HPT) when engineered as sub-2-μm particles that enable the revolutionary ACQUITY UPLC® and ACQUITY UPLC H-Class systems. These

advancements have empowered separation scientists in almost every industry to realize the dramatic business and scientific benefits of this transformative technology combination.

Responding to input received at many customer forums, Waters has developed the next evolution of particle technology: Charged Surface Hybrid (CSH™) Technology.

Unbonded BEH Particle

Start with the rugged,ultra-efficient, ethylene bridged

hybrid (BEH) particle

Apply Controlled Surface Charge

Add reproduciblelow-level charge to

particle surface

Bond and End Cap

Functionalize with appropriate bonded

phase chemistry

CSH™ Technology dramatically improves virtually all facets of LC column performance in acidic, low ionic

strength mobile phases that are commonly used in the chromatographic laboratory.

Advantages ofCSH Technology include:

- Superior peak shape forbasic compounds

- Increased loading capacity

- Rapid column re-equilibrationafter changing mobile-phase pH

- Improved batch-to-batchreproducibility

- Excellent stability at low pH

Efficiency is a powerful tool available to separation scientists. However, increasing the efficiency alone is often not sufficient to achieve the resolution necessary to produce the desired separation. Method development scientists turn to selectivity and/or retentivity in order to influence the chromatographic behavior of peaks in a separation. Selectivity can be manipulated through the use of pH, organic modifier and/or stationary phase. Mobile-phase pH and/or organic modifier are simple to measure and control, but how can a chromatographer know if one stationary phase is different from (or similar to) another?

When developing ACQUITY UPLC CSH and XSelect™ columns, one essential attribute designed into these families of UPLC® and HPLC columns was a wider selectivity range – different from each other and different from ACQUITY UPLC BEH and XBridge™ HPLC

columns. In order to do this, Waters developed several ways to measure selectivity differences/similarities between column chemistries. The Selectivity factor (S-value) measures how different (or similar) the selectivities of two column chemistries are under a given set of conditions. The value is determined by measuring and plotting the retention factors (k) of analytes run on two columns under the same chromatographic conditions. Higher selectivity values indicate a higher degree of orthogonality.

Visualizing, Quantifying and Creating Selectivity

ACQUITY UPLC CSH and XSelect Column Chemistries

CSH Chemistry

C18 Fluoro-Phenyl Phenyl-Hexyl

Ligand Type Trifunctional C18 Trifunctional Propylfluorophenyl Trifunctional C6 Phenyl

Available Particle Sizes 1.7, 3.5, 5 μm 1.7, 3.5, 5 μm 1.7, 3.5, 5 μm

Ligand Density* 2.3 μmol/m2 2.3 μmol/m2 2.3 μmol/m2

Carbon Load* 15 10 14

End-cap Style Proprietary None Proprietary

pH Range 1-11 1- 8 1-11

Temperature LimitsLow pH = 80 °C Low pH = 60 °C Low pH = 80 °C

High pH = 45 °C High pH = 45 °C High pH = 45 °C* Expected or Approximate Values

O SiO

OO Si

O

O

F F

F F

F O SiO

O

6

[ Chemistries Engineered to Provide Wider Range of Selectivities ]

The Selectivity factor (S-value) can be used to assess (and hence, help to create) orthogonality of selectivities between two different columns. Simply plot retention factors of one column versus another column, calculate the coefficient of determination (R2) and calculate the Selectivity factor as indicated. Larger values indicate higher orthogonality.

0

5

10

15

0 5 10 15 kg on XBridge C18

kg o

n XS

elec

t CSH

Pha

ses

CSH C18 CSH Fluoro-Phenyl CSH Phenyl-Hexyl

R2 = 0.76 S = 49

kg on XBridge C18

k g o

n XB

ridge

Pha

ses

R2 = 0.97 S = 16

0

5

10

15

0 5 10 15

BEH RP18BEH PhenylBEH C8

Selectivity (S) = 100 x 1-R2

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Columns Designed for Method Development and Method Transferability

ACQUITY UPLC CSH and XSelect columns offer method development scientists in all application areas the widest range of selectivities of any modern LC columns produced by Waters. This was accomplished without compromising such necessary performance attributes as superior peak shape for basic compounds, low column bleed, excellent batch-to-batch reproducibility, ultra-efficiencies and seamless transferability between different particle sizes and column formats. Many chromatographic laboratories are now part of multi-national/multi-site organizations that utilize LC systems from different vendors with varying LC platform configurations and detection modes. From a global business perspective, it is vital to be able to quickly and easily develop robust methods that are not only compatible with all modern chromatographic detection modes but are also transferable to laboratories and sites that may operate different LC system platforms. ACQUITY UPLC CSH and XSelect columns, as part of the ACQUITY UPLC H-Class total system solution, were strategically created for the 21st -century global chromatographic marketplace.

ACQUITY UPLC CSH C18

3.0 x 50 mm, 1.7 µmPart Number: 186005300

ACQUITY UPLC CSH Phenyl-Hexyl3.0 x 50 mm, 1.7 µmPart Number: 186005410

ACQUITY UPLC CSH Fluoro-Phenyl3.0 x 50 mm, 1.7 µmPart Number: 186005355

1 2

3

4

5

6

1 2

3 5

4

6

1 2

3,5

6

4

SCSH C18 = 18

SCSH C18 = 55SCSH Phenyl-Hexyl = 41

XSelect CSH Phenyl-Hexyl4.6 x 150 mm, 5 µmPart Number: 186005400

XSelect CSH Fluoro-Phenyl4.6 x 150 mm, 5 µm Part Number: 186005345

1 2

3

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5

6

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3 5

4

6

1

2

3,5

6

4

XSelect CSH Phenyl-Hexyl4.6 x 100 mm, 3.5 µmPart Number: 186005379

XSelect CSH Fluoro-Phenyl4.6 x 100 mm, 3.5 µmPart Number: 186005324

0.0 1.0 2.00.5 1.5 2.5 3.0 3.5 min 0 5 10 15 20 25 30 35 min0 2 4 6 8 10 12 14 16 min

α1,2 = 1.11

α3,5= 1.07

α6,4 = 1.04

XSelect CSH C18

4.6 x 150 mm, 5 µmPart Number: 186005290

α1,2 = 1.10

α3,5 = 1.07

α6,4 = 1.03

XSelect CSH C18


α1,2 = 1.10

α3,5 = 1.06

α6,4 = 1.03

1 2

3

4

5

6

1 2

3 5

4

6

1

2

3,5

6

4

Ease of Transferability (Identical α's Across Particle Sizes)

Wid

e Ra

nge

of S

elec

tivi

ties

(La

rge

S-Va

lues

)

8

[ Development and Transferability ]

ACQUITY UPLC CSH and XSelect columns offer not only a wide range of selectivity differences (large S-values), but also seamless and straightforward transferability (nearly identical α's) between UPLC, analytical HPLC and preparative HPLC particle and column configurations.



ACQUITY UPLC CSH Phenyl-Hexyl3.0 x 50 mm, 1.7 µmPart Number: 186005410

ACQUITY UPLC CSH Fluoro-Phenyl3.0 x 50 mm, 1.7 µmPart Number: 186005355

1 2

3

4

5

6

1 2

3 5

4

6

1 2

3,5

6

4

SCSH C18 = 18

SCSH C18 = 55SCSH Phenyl-Hexyl = 41

XSelect CSH Phenyl-Hexyl4.6 x 150 mm, 5 µmPart Number: 186005400

XSelect CSH Fluoro-Phenyl4.6 x 150 mm, 5 µm Part Number: 186005345

1 2

3

4

5

6

1 2

3 5

4

6

1

2

3,5

6

4

XSelect CSH Phenyl-Hexyl4.6 x 100 mm, 3.5 µmPart Number: 186005379

XSelect CSH Fluoro-Phenyl4.6 x 100 mm, 3.5 µmPart Number: 186005324

0.0 1.0 2.00.5 1.5 2.5 3.0 3.5 min 0 5 10 15 20 25 30 35 min0 2 4 6 8 10 12 14 16 min

α1,2 = 1.11

α3,5= 1.07

α6,4 = 1.04

XSelect CSH C18

4.6 x 150 mm, 5 µmPart Number: 186005290

α1,2 = 1.10

α3,5 = 1.07

α6,4 = 1.03

XSelect CSH C18


α1,2 = 1.10

α3,5 = 1.06

α6,4 = 1.03

1 2

3

4

5

6

1 2

3 5

4

6

1

2

3,5

6

4

Ease of Transferability (Identical α's Across Particle Sizes)

Compounds 1. Tolmetin (10 µg/mL)2. Naproxen (10 µg/mL)3. Fenoprofen (10 µg/mL)4. Indomethacin (10 µg/mL)5. Ibuprofen (100 µg/mL)6. Diclofenac (10 µg/mL)

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[ Development and Transferability ]

ACQUITY UPLC CSH and XSelect columns offer not only a wide range of selectivity differences (large S-values), but also seamless and straightforward transferability (nearly identical α's) between UPLC, analytical HPLC and preparative HPLC particle and column configurations.

Compounds1. Doxepin2. Desipramine3. Imipramine4. Nortriptyline5. Amitriptyline6. Trimipramine

LC ConditionsColumns: ACQUITY UPLC CSH C18, 2.1 x 50 mm, 1.7 μm Part Number: 186005296 Kinetex C18, 2.1 x 50 mm, 1.7 µmMobile Phase A: waterMobile Phase B: acetonitrile Mobile Phase C: 2% formic acid in waterMobile Phase D: 2% trifluoroacetic acid in water

Injection Volume: 5 μLSample Diluent: waterSample Concentration: 10 µg/mL eachColumn Temperature: 40 °CWash Solvent: 50/50 acetonitrile/water Purge Solvent: 50/50 acetonitrile/waterDetection: UV @ 254 nmSampling Rate: 20 points/secFilter Time Constant: 0.1 secSystem: ACQUITY UPLC H-Class with ACQUITY UPLC PDA and SQD

MS ConditionsCapillary: 3.0 kV (ES+)Cone: 30 VExtractor: 3 VDesolvation Temperature: 350 °CSource Temperature: 120 °CDesolvation Gas: 800 L/hrScan Range: 100 to 600 m/zScan Rate: 5000 Da/sec

Gradient: Time (min)

Flow(mL/min)

%A %B %C/%D Curve

Initial 0.6 72.5 25 2.5 Initial2.0 0.6 62.5 35 2.5 63.0 0.6 2.5 95 2.5 63.1 0.6 72.5 25 2.5 65.0 0.6 72.5 25 2.5 6

1

2 3

4

5 6

Inte

nsity

5.0 x 107

1.0 x 108

1.5 x 108

0.00

0.50.0 1.0 1.5 2.0 2.5 min


0.05% formic acidPc = 47

1.3X peak capacity (Pc)4.8X peak height

Inte

nsity

5.0 x 107

1.0 x 108

1.5 x 108

0.00

0.50.0 1.0 1.5 2.0 2.5 min

1 2 3 4 5

6

Kinetex C18

0.05% TFAPc = 36

Kinetex™ C18


1 2 3 4

5 6

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0.10

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0.10

2.6X peak capacity (Pc)2.4X peak height

0.0 0.5 1.0 1.5 2.0 2.5 min

UV Chromatographic Performance with Formic Acid as Additive MS Signal Response at Maximum Peak Capacity (Pc)

[ Eliminate the Need for TFA ]

TFA Performance Without TFA Suppression

Trifluoroacetic acid (TFA) is commonly used as an acidic mobile-phase additive/modifier due to the chromatographic benefits that it provides, especially when analyzing basic compounds. Historically TFA is considered a ‘peak sharpening agent’ and offers improved peak shape for basic compounds. However, TFA use does not come without its drawbacks. TFA is considered an ion-pairing agent and as such, once an LC column is exposed to TFA, it is difficult to completely remove it from the stationary phase. This makes method development difficult since a column is often used with multiple buffers/additives at different pH values. As a result, it can be difficult to determine if the optimal chromatographic conditions may be a result of previous TFA exposure. More importantly, as the use of electrospray ionization-mass spectrometry (ESI-MS)

in the chromatographic laboratory is becoming more common place, alternatives to TFA are being investigated since TFA can cause some analyte signal suppression in electrospray positive (ES+) ionization at concentrations exceeding 0.01% and severe analyte signal suppression in electrospray negative (ES-) ionization.

CSH Technology provides TFA peak shape performance without the use of TFA (and its associated disadvantages). Improved peak shape performance is realized with a more MS-friendly mobile-phase additive such as formic acid (e.g., ≤ 0.1% HCOOH). Further, because formic acid does not exhibit the lingering chromatographic effects of an ion-pairing agent, method development is faster and more straightforward.

With CSH Technology, the need for TFA, and the associated drawbacks it brings to the modern LC/MS laboratory, has been all but eliminated. Designed for optimal chromatographicperformance with simple, volatile, MS-compatible mobile phases, ACQUITY UPLC CSH and XSelect columns are a true evolution in LC column performance.

Comparative separations may not be representative of all applications.

Under these test conditions the Kinetex column is exhibiting peak shapes indicative of column overload.

[ Trace Impurity Separation ]

The increased and improved loadability that is possible with CSH Technology permits the separation, identification and quantitation of closely eluting impurities or degradants. In this example, a high concentration (0.5 mg/mL)of imipramine was repeatedly injected while the concentration of amitriptyline was decreased with each injection.

AU

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0.02

0.04

0.06

0.08

Imip

ram

ine

Imip

ram

ine

2.0%

1.0%

0.5%

0.1%

2.0% 1.0%

0.5% 0.1%

Amitriptyline

Imipramine concentration held constant at 0.5 mg/mL

Amitriptyline

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min


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Kinetex C18

LC ConditionsColumns: ACQUITY UPLC CSH C18, 2.1 x 50 mm, 1.7 μm Part Number: 186005296 Kinetex C18, 2.1 x 50 mm, 1.7 µmMobile Phase A: waterMobile Phase B: acetonitrile Mobile Phase C: 2% formic acid in water

Injection Volume: 5 μLSample Diluent: waterSample Concentration: imipramine: 0.5 mg/mL; amitriptyline: as indicated (% of imipramine) Column Temperature: 40 °CWash Solvent: 50/50 acetonitrile/water Purge Solvent: 50/50 acetonitrile/waterDetection: UV @ 254 nmSampling Rate: 20 points/secFilter Time Constant: 0.1 secSystem: ACQUITY UPLC H-Class with ACQUITY UPLC PDA and SQD

MS ConditionsCapillary: 3.0 kV (ES+)Cone: 30 VExtractor: 3 VDesolvation Temperature: 350 °CSource Temperature: 120 °CDesolvation Gas: 800 L/hrScan Range: 100 to 600 m/zScan Rate: 5000 Da/sec

Gradient: Time(min)

Flow(mL/min)

%A %B %C Curve

Initial 0.6 70 25 5 Initial2.0 0.6 60 35 5 63.0 0.6 0 95 5 63.1 0.6 70 25 5 65.0 0.6 70 25 5 6

(i.e., peak width increases, efficiency/peak capacity decreases, etc.) as a function of increasing analyte concentration. If one can create a column with an increased column overload concentration threshold, one can increase the sensitivity and utility of the assay. A narrower ‘main’ analyte peak imparts additional chromatographic ‘space’ for the separation and quantitation of closely-eluting, trace-level impurities or degradants. The increased loading capacity built into the CSH Technology-powered ACQUITY UPLC CSH and XSelect columns provides sharper, narrower peaks for basic compounds under the acidic, low ionic strength mobile-phase conditions most commonly used in the aforementioned application areas.

Increased Analytical Loadability

The ability to inject large amounts of material onto analytical-scale chromatographic columns is important in application areas such as stability indicating assays; metabolite ID and forced degradation studies; API impurity profiling; and genotoxic impurities assessments. In these analytical-column scale chromatographic assays, a large bolus of one or more analyte(s) of interest is/are injected and the researcher determines the presence or absence of small amounts of related compounds that can be benign, toxic or deadly.

In these types of analyses, the peak(s) of interest are usually injected at a concentration that ‘overloads’ the column. Overload conditions are when the peak shape degrades

Comparative separations may not be representative of all applications.

Using CSH Technology throughout the entire process, methods can be developed quickly with ACQUITY UPLC CSH columns and UPLC Technology and then transferred to preparative-scale OBD XSelect columns for isolation and purification.The purity of the isolated fraction(s) can then be measured/confirmed using ACQUITY UPLC CSH columns and UPLC Technology.

[ Scalability, Loadability and Stability ]

3. Run preparative separation using focused gradient

4. Collect and isolate fraction(s) of interest

5. Confirm fraction purity using UPLC Technology

1. Develop method using UPLC Technology

2. Scale UPLC separation to preparative column scale

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0.00

0.50

1.00

1.50

Pooled purified fractionsDiphenhydramine = 100% purity

0.00

0.50

1.00

1.50

AU

Crude mixDiphenhydramine = 56.3% purity

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

2 3 4 5 6 7 8 9 10 min

0.00

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1.50

1

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1

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3 2.00

Prep Purification100 mg total load

Target Analyte

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AU

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1.00

1.50


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AU


0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

2 3 4 5 6 7 8 9 10 min

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Target Analyte

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0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 min

2 3 4 5 6 7 8 9 10 min

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Target Analyte

AU

AU

Columns Designed for Isolation and Purification

CSH Technology, especially when combined with the analytical method development capabilities of UPLC Technology and the industry-leading column lifetimes offered by Optimum Bed Density (OBD™) preparative columns, may have the greatest benefits to isolation and purification scientists. Why? CSH Technology offers the highest loading of basic compounds separated under the volatile, acidic, low pH mobile-phase conditions often preferred by isolation and purification scientists. This increased loadability allows for the use of a narrower (lower volume) preparative column thereby reducing solvent consumption and fraction volumes.

The scalability built into ACQUITY UPLC CSH and XSelect columns permits the use of UPLC Technology as a powerful method development tool for the isolation and purification scientist. How can chromatographic separation platforms that appear to be on opposite ends of the analysis scale spectrum (microbore vs. preparative) complement each other? Again, the answer lies in scalability and CSH Technology’s resistance to ‘pH switching’ column performance degradation. Fast, robust analytical-scale separations can be easily and quickly developed using UPLC Technology, ACQUITY UPLC CSH columns and simple mobile phase additives and then straightforwardly scaled and transferred to an XSelect OBD preparative column. In addition, the purity check of the isolated fraction(s) and/or structural analysis of isolated impurities can be determined using UPLC/MS.

No other manufacturer can offer the isolation and purification scientist the selectivity, loadability and scalability of CSH Technology with the throughput, sensitivity and transferable analytical-scale method development of UPLC Technology.

Preparative ConditionsColumn: XSelect CSH Prep C18 OBD, 19 x 100 mm, 5 μm Part Number: 186005421Mobile Phase A: 0.1% formic acid in waterMobile Phase B: 0.1% formic acid in acetonitrile

Injection Volume: 1.25 mLSample Concentration: 80 mg/mL for crude mixDiphenhydramine: 40 mg/mLOxybutynin: 16 mg/mLTerfenadine : 24 mg/mLSample Diluent: DMSOColumn Temperature: 40 °CWeak Needle Wash: 95/5 water/methanol Strong Needle Wash: 95/5 methanol/waterDetection: UV @ 220 nmSampling Rate: 1 point/secFilter Time Constant: 1 secSystem: Waters 2525 Binary Gradient Module, 2767 Sample Manager, Column Fluidics Organizer, 2996 Photodiode Array Detector, ZQ™ Mass Spectrometer


Flow(mL/min)

%A %B Curve

Initial 25 95 5 Initial1.21 25 95 5 61.71 25 78.8 21.2 67.02 25 70.8 29.2 67.21 25 5 95 68.21 25 5 95 68.31 25 95 5 613.21 25 95 5 6

LC ConditionsColumns: ACQUITY UPLC CSH C18, 2.1 x 50 mm, 1.7 μm Part Number: 186005296Mobile Phase A: 0.1% formic acid in waterMobile Phase B: 0.1% formic acid in acetonitrile

Injection Volume: 1 μLSample Concentration: 800 µg/mL for crude mixSample Diluent: 50/50 methanol/waterColumn Temperature: 40 °CWeak Needle Wash: 95/5 water/acetonitrile Strong Needle Wash: 95/5 acetonitrile/waterDetection: UV @ 220 nmSampling Rate: 20 points/secFilter Time Constant: 0.1 secSystem: ACQUITY UPLC System with ACQUITY UPLC PDA


Flow(mL/min)

%A %B Curve

Initial 0.9 95 5 Initial2.0 0.9 5 95 62.5 0.9 5 95 62.51 0.9 95 5 63.50 0.9 95 5 6

Compounds1. Diphenhydramine2. Oxybutynin3. Terfenadine

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[ Ordering Information ]

XSelect Analytical Columns

Dimension Type Particle Size C18 Fluoro-Phenyl Phenyl-Hexyl

1.0 x 50 mm Column 3.5 µm 186005249 186005304 1860053591.0 x 100 mm Column 3.5 µm 186005250 186005305 1860053601.0 x 150 mm Column 3.5 µm 186005251 186005306 1860053612.1 x 10 mm Guard1 3.5 µm 186005252 186005307 1860053622.1 x 20 mm IS™ Column 3.5 µm 186005253 186005308 1860053632.1 x 30 mm Column 3.5 µm 186005254 186005309 1860053642.1 x 50 mm Column 3.5 µm 186005255 186005310 1860053652.1 x 100 mm Column 3.5 µm 186005256 186005311 1860053662.1 x 150 mm Column 3.5 µm 186005257 186005312 1860053673.0 x 20 mm Guard2 3.5 µm 186005258 186005313 1860053683.0 x 20 mm IS Column 3.5 µm 186005259 186005314 1860053693.0 x 30 mm Column 3.5 µm 186005260 186005315 1860053703.0 x 50 mm Column 3.5 µm 186005261 186005316 1860053713.0 x 100 mm Column 3.5 µm 186005262 186005317 1860053723.0 x 150 mm Column 3.5 µm 186005263 186005318 1860053734.6 x 20 mm Guard2 3.5 µm 186005264 186005319 1860053744.6 x 20 mm IS Column 3.5 µm 186005265 186005320 1860053754.6 x 30 mm Column 3.5 µm 186005266 186005321 1860053764.6 x 50 mm Column 3.5 µm 186005267 186005322 1860053774.6 x 75 mm Column 3.5 µm 186005268 186005323 1860053784.6 x 100 mm Column 3.5 µm 186005269 186005324 1860053794.6 x 150 mm Column 3.5 µm 186005270 186005325 186005380

2.1 x 10 mm Guard1 5 µm 186005271 186005326 1860053812.1 x 20 mm IS Column 5 µm 186005272 186005327 1860053822.1 x 30 mm Column 5 µm 186005273 186005328 1860053832.1 x 50 mm Column 5 µm 186005274 186005329 1860053842.1 x 100 mm Column 5 µm 186005275 186005330 1860053852.1 x 150 mm Column 5 µm 186005276 186005331 1860053863.0 x 20 mm Guard2 5 µm 186005277 186005332 1860053873.0 x 20 mm IS Column 5 µm 186005278 186005333 1860053883.0 x 30 mm Column 5 µm 186005279 186005334 1860053893.0 x 50 mm Column 5 µm 186005280 186005335 1860053903.0 x 100 mm Column 5 µm 186005281 186005336 1860053913.0 x 150 mm Column 5 µm 186005282 186005337 1860053923.0 x 250 mm Column 5 µm 186005283 186005338 1860053934.6 x 20 mm IS Column 5 µm 186005284 186005339 1860053944.6 x 20 mm Guard2 5 µm 186005285 186005340 1860053954.6 x 30 mm Column 5 µm 186005286 186005341 1860053964.6 x 50 mm Column 5 µm 186005287 186005342 1860053974.6 x 75 mm Column 5 µm 186005288 186005343 1860053984.6 x 100 mm Column 5 µm 186005289 186005344 1860053994.6 x 150 mm Column 5 µm 186005290 186005345 1860054004.6 x 250 mm Column 5 µm 186005291 186005346 186005401

XSelect Preparative Columns

Dimension Type Particle Size C18 Fluoro-Phenyl Phenyl-Hexyl

10 x 10 mm Guard 5 µm 186005491 186005498 18600550510 x 50 mm Column 5 µm 186005414 186005427 18600544010 x 100 mm Column 5 µm 186005415 186005428 18600544110 x 150 mm Column 5 µm 186005416 186005429 18600544210 x 250 mm Column 5 µm 186005417 186005430 18600544319 x 10 mm Guard 5 µm 186005418 186005431 186005444OBD 19 x 50 mm Column 5 µm 186005420 186005433 186005446OBD 19 x 100 mm Column 5 µm 186005421 186005434 186005447OBD 19 x 150 mm Column 5 µm 186005422 186005435 186005448OBD 19 x 250 mm Column 5 µm 186005492 186005499 186005506OBD 30 x 50 mm Column 5 µm 186005423 186005436 186005520OBD 30 x 75 mm Column 5 µm 186005424 186005437 186005450OBD 30 x 100 mm Column 5 µm 186005425 186005438 186005451OBD 30 x 150 mm Column 5 µm 186005426 186005439 186005452OBD 30 x 250 mm Column 5 µm 186005493 186005500 186005507OBD 50 x 50 mm Column 5 µm 186005494 186005501 186005508OBD 50 x 100 mm Column 5 µm 186005495 186005502 186005509OBD 50 x 150 mm Column 5 µm 186005496 186005503 186005510OBD 50 x 250 mm Column 5 µm 186005497 186005504 186005511

XSelect Method Validation Kits

Dimension Particle Size C18 Fluoro-Phenyl Phenyl-Hexyl

2.1 x 100 mm 3.5 µm 186005538 186005549 1860055603.0 x 100 mm 3.5 µm 186005539 186005550 1860055613.0 x 150 mm 3.5 µm 186005540 186005551 1860055624.6 x 100 mm 3.5 µm 186005541 186005552 1860055634.6 x 150 mm 3.5 µm 186005542 186005553 186005564

2.1 x 150 mm 5 µm 186005543 186005554 1860055653.0 x 100 mm 5 µm 186005544 186005555 1860055663.0 x 150 mm 5 µm 186005545 186005556 1860055674.6 x 100 mm 5 µm 186005546 186005557 1860055684.6 x 150 mm 5 µm 186005547 186005558 1860055694.6 x 250 mm 5 µm 186005548 186005559 186005570

14

1 Requires Universal SentryTM Guard Holder - 2.1 x 10 mm WAT0979582 Requires Universal Sentry Guard Holder - 3.0 x 20 mm/4.6 x 20 mm WAT046910

UPLC Column Hardware

Description Part No.

Three 0.2 µm Inlet/Outlet Frits for 3.0 mm ID UPLC Columns 700004790Three 0.2 µm Inlet/Outlet Frits for 2.1 mm ID UPLC Columns 700003776Three 0.2 µm Inlet/Outlet Frits for 1.0 mm ID UPLC Columns 700003775One Inlet End Nut for 3.0 mm ID UPLC Column 700004792One Outlet End Nut for 3.0 mm ID UPLC Column 700004791One Inlet End Nut for 2.1 mm ID UPLC Column 700003779One Outlet End Nut for 2.1 mm ID UPLC Column 700003780One Inlet End Nut for 1.0 mm ID UPLC Column 700003777One Outlet End Nut for 1.0 mm ID UPLC Column 700003778

ACQUITY UPLC CSH Columns

Chemistry Particle Size Dimension 1 Pack 3 Pack

C18 1.7 µm 1.0 x 50 mm 186005292 176002136C18 1.7 µm 1.0 x 100 mm 186005293 176002137C18 1.7 µm 1.0 x 150 mm 186005294 176002138C18 1.7 µm 2.1 x 30 mm 186005295 176002139C18 1.7 µm 2.1 x 50 mm 186005296 176002140C18 1.7 µm 2.1 x 100 mm 186005297 176002141C18 1.7 µm 2.1 x 150 mm 186005298 176002142C18 1.7 µm 3.0 x 30 mm 186005299 176002143C18 1.7 µm 3.0 x 50 mm 186005300 176002144C18 1.7 µm 3.0 x 100 mm 186005301 176002145C18 1.7 µm 3.0 x 150 mm 186005302 176002146

Fluoro-Phenyl 1.7 µm 1.0 x 50 mm 186005349 176002150Fluoro-Phenyl 1.7 µm 1.0 x 100 mm 186005347 176002148Fluoro-Phenyl 1.7 µm 1.0 x 150 mm 186005348 176002149Fluoro-Phenyl 1.7 µm 2.1 x 30 mm 186005350 176002151Fluoro-Phenyl 1.7 µm 2.1 x 50 mm 186005351 176002152Fluoro-Phenyl 1.7 µm 2.1 x 100 mm 186005352 176002153Fluoro-Phenyl 1.7 µm 2.1 x 150 mm 186005353 176002154Fluoro-Phenyl 1.7 µm 3.0 x 30 mm 186005354 176002155Fluoro-Phenyl 1.7 µm 3.0 x 50 mm 186005355 176002156Fluoro-Phenyl 1.7 µm 3.0 x 100 mm 186005356 176002157Fluoro-Phenyl 1.7 µm 3.0 x 150 mm 186005357 176002158

Phenyl-Hexyl 1.7 µm 1.0 x 50 mm 186005404 176002161Phenyl-Hexyl 1.7 µm 1.0 x 100 mm 186005402 176002159Phenyl-Hexyl 1.7 µm 1.0 x 150 mm 186005403 176002160Phenyl-Hexyl 1.7 µm 2.1 x 30 mm 186005405 176002162Phenyl-Hexyl 1.7 µm 2.1 x 50 mm 186005406 176002163Phenyl-Hexyl 1.7 µm 2.1 x 100 mm 186005407 176002164Phenyl-Hexyl 1.7 µm 2.1 x 150 mm 186005408 176002165Phenyl-Hexyl 1.7 µm 3.0 x 30 mm 186005409 176002166Phenyl-Hexyl 1.7 µm 3.0 x 50 mm 186005410 176002167Phenyl-Hexyl 1.7 µm 3.0 x 100 mm 186005411 176002168Phenyl-Hexyl 1.7 µm 3.0 x 150 mm 186005412 176002169

ACQUITY UPLC CSH VanGuard™ Pre-Column Packs

Chemistry Particle Size Dimension 3 Pack

C18 1.7 µm 2.1 x 5 mm 186005303

Fluoro-Phenyl 1.7 µm 2.1 x 5 mm 186005358

Phenyl-Hexyl 1.7 µm 2.1 x 5 mm 186005413

ACQUITY UPLC CSH Method Development Kits

Package NameQty/Pk

ChemistriesParticle Size(s)

Dimension Part No.

Maximum Selectivity UPLCMethod Development Kit

4/pkCSH C18, Phenyl-Hexyl, Fluoro-Phenyl 1.7 µm

2.1 x 50 mm 176002123HSS C18 SB 1.8 µm



2.1 x 100 mm 176002124HSS C18 SB 1.8 µm



3.0 x 50 mm 176002125HSS C18 SB 1.8 µm



3.0 x 100 mm 176002126HSS C18 SB 1.8 µm

Maximum Selectivity RP and HILICUPLC Method Development Kit

4/pkCSH C18, Phenyl-Hexyl,

Fluoro-Phenyl, BEH Amide1.7 µm 2.1 x 50 mm 176002127










ACQUITY UPLC CSH Method Validation Kits

Dimension Particle Size C18 Fluoro-Phenyl Phenyl-Hexyl

2.1 x 50 mm 1.7 µm 186005571 186005575 1860055792.1 x 100 mm 1.7 µm 186005572 186005576 1860055803.0 x 50 mm 1.7 µm 186005573 186005577 1860055813.0 x 100 mm 1.7 µm 186005574 186005578 186005582

15

ACQUITY UPLC CSH Method Transfer Kits

Package Name Description Part No.

CSH C18 1.7 µm to 5 µmMethod Transfer Kit

XSelect CSH C18 4.6 x 150 mm, 5 µm ACQUITY UPLC CSH C18 2.1 x 50 mm, 1.7 µm

186005529

CSH Fluoro-Phenyl 1.7 µm to 5 µmMethod Transfer Kit

XSelect CSH Fluoro-Phenyl 4.6 x 150 mm, 5 µm ACQUITY UPLC CSH Fluoro-Phenyl 2.1 x 50 mm, 1.7 µm

186005531

CSH Phenyl-Hexyl 1.7 µm to 5 µmMethod Transfer Kit

XSelect CSH Phenyl-Hexyl 4.6 x 150 mm, 5 µm ACQUITY UPLC CSH Phenyl-Hexyl 2.1 x 50 mm, 1.7 µm

186005530

CSH C18 1.7 µm to 3.5 µmMethod Transfer Kit

XSelect CSH C18 4.6 x 100 mm, 3.5 µm ACQUITY UPLC CSH C18 2.1 x 50 mm, 1.7 µm

186005532

CSH Fluoro-Phenyl 1.7 µm to 3.5 µmMethod Transfer Kit

XSelect CSH Fluoro-Phenyl 4.6 x 100 mm, 3.5 µm ACQUITY UPLC CSH Fluoro-Phenyl 2.1 x 50 mm, 1.7 µm

186005534

CSH Phenyl-Hexyl 1.7 µm to 3.5 µmMethod Transfer Kit

XSelect CSH Phenyl-Hexyl 4.6 x 100 mm, 3.5 µm ACQUITY UPLC CSH Phenyl-Hexyl 2.1 x 50 mm, 1.7 µm

186005533

CSH C18 1.7 µm to 3.5 µmHigh Rs Method Transfer Kit

XSelect CSH C18 4.6 x 150 mm, 3.5 µm ACQUITY UPLC CSH C18 2.1 x 100 mm, 1.7 µm

186005535

CSH Fluoro-Phenyl 1.7 µm to 3.5 µm High Rs Method Transfer Kit

XSelect CSH Fluoro-Phenyl 4.6 x 150 mm, 3.5 µm ACQUITY UPLC CSH Fluoro-Phenyl 2.1 x 100 mm, 1.7 µm

186005537

CSH Phenyl-Hexyl 1.7 µm to 3.5 µmHigh Rs Method Transfer Kit

XSelect CSH Phenyl-Hexyl 4.6 x 150 mm, 3.5 µm ACQUITY UPLC CSH Phenyl-Hexyl 2.1 x 100 mm, 1.7 µm

186005536

©2011 Waters Corporation. Waters, CSH, ACQUITY UPLC, XSelect, UPLC, XBridge,

OBD, ZQ, IS, VanGuard, Sentry and The Science of What’s Possible are trademarks of

Waters Corporation. Kinetex is a trademark of Phenomenex, Inc.

April 2011 720003928EN KK-FP

Sales Offices

The quality management system of Waters’ manufacturing facilitiesin Taunton, Massachusetts and Wexford, Ireland complies with the International Standard ISO 9001:2008 Quality Management and Quality Assurance Standards. Waters’ quality management system is periodically audited by the registering body to ensure compliance.

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