(a)
DESCRIPTION
(a). ( b ). PDC1. 3.5. WT. 3. npt1∆. 2.5. 2. NAD/NADH ratio. Relative expression. 1.5. 1. 0.5. 0. log. 3. 6. 9. 12. log. 3. 6. 9. 12. hrs post log phase. hrs post log phase. ( c ). ( d ). [0- 1093]. 500 bp. Input. WT. [0- 666]. sir2∆. hst1∆. Sir2. x. sum1∆. - PowerPoint PPT PresentationTRANSCRIPT
(a) (b)
00.5
11.5
22.5
33.5
log 3 6 9 12
NA
D/N
AD
H r
atio
0
0.2
0.4
0.6
0.8
1
log 3 6 9 12
Re
lativ
e e
xpre
ssio
n
PDC1
PDC5
Input
Sir2
Hst1
Sum1
(d)
(e)
log 3 6 9 12
Re
lativ
e e
xpre
ssio
n
D
D
PDC5
00.10.20.30.40.50.60.70.80.9
1WTsir2∆
npt1∆hst1∆
hrs post log phasehrs post log phase
hrs post log phase
(f)
log 3 6 9 12
Re
lativ
e e
xpre
ssio
n
PDC1
WTsir2∆sir2∆+vecsir2∆+WTsir2∆+H364Y
hrs post log phase
WTnpt1∆
[0- 1093]
[0- 666]
[0- 500]
[0- 565]
(c)
0
5
10
15
20
25
Glu
cose
(m
g/m
l)
3 6 9 12 15 18 hours
x
WTsir2∆hst1∆sum1∆
0
0
0.4
0.8
1.2
1.6
500 bp