a (re-) introduction to biocontrol & phd project update
TRANSCRIPT
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Project Update:Population Genomics of Natural EnemiesGSS | June 14th, 2016Kim Ferguson
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Biocontrol: a brief (re-)introductionBiological control, biocontrol, natural enemies, classical vs conservation vs others...
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What is biocontrol?
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Types of Biocontrol
Natural
Conservation
Classical
Augmentative
Definitions based on van Lenteren, in BioControl (2012)
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Agriculture & Biocontrol:Integrated Pest Management (IPM)
Che
mical
Biological
Physical-Mechanical
Cultural
Toxic
ity Two important concepts:- Economic Injury Level (EIL)- Control, not eradication
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Current events: Mosquitos and Biocontrol
What are the consequences of getting rid of mosquitos entirely?
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Future of Biocontrol?
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Future of Biocontrol?
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Breeding Invertebrates for Next Generation Biocontrol
My project within BINGO
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Quick recap: what is my project??• : improving natural enemies used in
biological control via next generation techniques
• RP1: Population Genomics of Natural Enemies• My own goal: population genomics of commercial vs wild
populations
• 3 species »» 3 genomes »» 3 parallel collaborations
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Nesidiocoris tenuis
Used against:- Whitefly- Tuta absoluta
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Nesidiocoris tenuis• Likely faces severe inbreeding pressure as well as laboratory
adaptation• Unknown source history?• Lack of stock renewal
• Work with RP10 (Valencia, Spain) in terms of how genomics can inform reducing negative effects of N. tenuis on tomato crops
• Progress: Should know by the end of the week if females in established ‘families’ laid eggs to begin sib-mating inbreeding scheme
• Upcoming: This is long-term rearing, so more rearing!
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Trichogramma brassicae
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Trichogramma spp.
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Trichogramma brassicae
• Commercial stock is renewed from various local populations annually, so little risk of inbreeding
• Work with RP12 (Bremen, Germany) for strain-specific markers
• Progress: Obtained parthenogenetic strain from Germany, will speed up the road to sequencing as compared to inbreeding
• Upcoming: Breeding and then harvesting them for sequencing in July
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Amblyseius swirskii
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Amblyseius swirskii
Used against:- Whitefly (eggs & larvae)- Thrips (eggs & larvae)
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Amblyseius swirskii• Likely faces severe inbreeding pressure and lab adaptation• Initial bottlenecks• Lack of stock renewal
• Work with RP3 (Valencia, Spain) in terms of how the genomic information can inform performance on tomatoes as compared to wild populations
• Progress: Scaling down industrial rearing to small-scale, isofemale rearing is proving... Difficult.
• Upcoming: Hopefully, on track for finishing inbreeding in September/October.
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More information?BINGO is on Twitter, @bingo_itn
You can like Breeding Invertebrates for Next Generation Biocontrol on Facebook
We even have a newsletter: www.bingo-itn.eu for more information and sign-up
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Acknowledgements• Bart Pannebakker, Eveline Verhulst, and Bas Zwaan• Gabriella Bukovinszkiné Kiss and Nina Fatouros• BINGO colleagues• Everyone here at the Lab of Genetics• Kirsten Oude Lenferink and everyone at Koppert BV• AMW Nützlinge• And you!
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Image Credits• Slide 3, clockwise from top left:
• Nematode, University of Maryland• Nanophyes marmoratus, BioLib,
http://www.biolib.cz/• Virus, Shutterstock• Cane toad, The Courier Mail Australia• Mucuna pruriens, Florist Taxonomy,
http://www.floristtaxonomy.com/
• Slide 4, clockwise from top left:• Ladybug, Shutterstock• Bug Hotel, NIOO-KNAW• Trichogramma brassicae, Bugs In The
Picture, bugsinthepicture.com• Slide 5: adapted from the US Environmental
Protection Agency
• Slide 10, R-L: • Trichogramma brassicae, Bugs In The
Picture, bugsinthepicture.com• Nesidiocoris tenuis, Jarmo Holopaeinn• Amblysieus swirskii, Koppert BV
• Slide 11 (and throughout):• Nesidiocoris tenuis, Kim Ferguson
• Slide 13 (and throughout):• Trichogramma brassicae, care of Sophie
Chattington @ Uni Bremen• Slide14 (all): Shutterstock• Slide 16 (and throughout):
• Swirski-Plus packet, Kim Ferguson