name of the speaker maria isabel trillas (phd biology ... · maria isabel trillas (phd biology,...

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Communication between plants and Trichoderma spp Name of the speaker: Professor Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist EU Women Innovator Prize 2017) Company: Biocontrol Technologies, S.L. Topic: 3. Microbials Summary: Reduction of plant diseases by Trichoderma spp. goes further than antagonism /competition with pathogens, they also enhance plant resistance. In addition, plants play an active role in the maintenance of the beneficial interaction. Trillas, M.I ., Segarra G., Casanova, E., Fernández, E. [email protected] 1 *PhD Biology, Full time lecturer Universitat de Barcelona, co-founder Biocontrol Technologies, finalist EU Women Innovator Prize 2017)

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Page 1: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

Communication between plants and Trichoderma spp

Name of the speaker: Professor Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist EU WomenInnovator Prize 2017)Company: Biocontrol Technologies, S.L.

Topic: 3. Microbials

Summary: Reduction of plant diseasesby Trichoderma spp. goes further than antagonism /competition with pathogens, they also enhance plant resistance. In addition, plants play an active role in the maintenance of the beneficial interaction.

Trillas, M.I., Segarra G., Casanova, E., Fernández, [email protected]

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*PhD Biology, Full time lecturer Universitat de Barcelona, co-founder BiocontrolTechnologies, finalist EU Women Innovator Prize 2017)

Page 2: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

Communication between plants and Trichoderma spp

Name of the speaker: Professor Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist EU WomenInnovator Prize 2017)Company: Biocontrol Technologies, S.L.

Topic: 3. Microbials

Summary: Reduction of plant diseasesby Trichoderma spp. goes further than antagonism /competition with pathogens, they also enhance plant resistance. In addition, plants play an active role in the maintenance of the beneficial interaction.

Trillas, M.I., Segarra G., Casanova, E., Fernández, [email protected]

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Page 3: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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R. James Cook – Kenneth F. Baker. Chapter 6. The host and biological control. In: The Nature and Practice of Biological Control of Plant Pathogens. 1983. The American Phytopathological Society.

Page 4: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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Page 5: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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Page 6: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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Page 7: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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REDUCTION Population of PATHOGEN

Changes at according the

population of T34 applied to the roots

ISR /SAR

At 107 cfu/ml

JA SA Peroxidase

17 proteins 11 proteins

regulated

+ Pseudomonas syringae pv. lachrymans5 x107 spores/ml

T34 1x105 or 1x106 or 1x107

cfu/ml growth media

Page 8: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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+ P. syringae pv tomato2,5 x107 cfu/ml

+ Hyaloperonospora parasitica5 x104 spores/ml

+ Plectosphaerella cucumerina5 x106 spores/ml

DISEASE REDUCTION BROAD SPECTRUM

ISR

T34 populations 10 fold

PEROXIDASE CHITINASE

CALLOSEDeposition

T34 1x105 cfu/ml growth media

priming

Page 9: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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+ Hyaloperonospora parasitica5 x104 spores/ml

T34 1x105 cfu/ml growth media

ISR

Arabidopsis mutants: sid2-1, npr1-1 and myb72-1

MYB72, NPR1required

priming

LOX 2 gen(LIPOXYGENASE2)

Page 10: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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+ B. cinerea1x105 conidia/ml

E. Fernández, G. Segarra and M.I. Trillas. Physiological effects of the induction of resistance by compost or Trichoderma asperellum strain T34 against Botrytis cinerea in tomato. 2014. Biological Control 78:77-85.

ISR

DISEASE SEVERITY (35)%

IMPROVED PLANT GROWTH

Nutrient uptakeMg, P, Cu, Bo

δ13C, δ15NBetter allocation

Leaf area Height Biomass T34 1x105 cfu/ml growth media

Page 11: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

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+ B. cinerea1x105 conidia/ml

T34 1x105 cfu/ml growth media

ISR

DISEASE SEVERITY (25)%

Changes (200 metabolite

peaks) in the secretion pattern root exudates

gluconic acid Inositol sucrose

Page 12: Name of the speaker Maria Isabel Trillas (PhD Biology ... · Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist

Communication between plants and Trichoderma spp

Name of the speaker: Professor Maria Isabel Trillas (PhD Biology, Full time lecturer University of Barcelona, co-founder Biocontrol Technologies, finalist EU WomenInnovator Prize 2017)Company: Biocontrol Technologies, S.L.

Topic: 3. Microbials

Summary: Reduction of plant diseasesby Trichoderma spp. goes further than antagonism /competition with pathogens, they also enhance plant resistance. In addition, plants play an active role in the maintenance of the beneficial interaction.

Trillas, M.I., Segarra G., Casanova, E., Fernández, [email protected]

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