a novel, natural anti-microbial product for use as an ... · a novel, natural anti-microbial...

Willem Ravensberg R&D Microbials

Koppert Biological Systems

Berkel & Rodenrijs, the Netherlands

A novel, natural anti-microbial product for use as an agricultural

bactericide and fungicide

1st Annual Biocontrol Industry Meeting, October 23-24, 2006, Lucerne, Switzerland

Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericide LP against virusesCompatibility Conclusions

What is the Lactoperoxidase system?

Occurs in animals and humansImportant non-immune defence systemActive system in milk, saliva, tears, etc.Used in food products and oral care productsKoppert and DMV/Campina have developed it as a natural fungicide/bactericide

Natural system based on:- 1 enzyme + 2 substrate components:

lactoperoxidase, SCN- and H2O2

Based on the formation of reactive oxygen species that inhibit or kill bacteria FAO: milk preservation: addition of SCN- and H2O2

What is the LP system?

LP in oral care products

Toothpaste (Zendium, Biotene)

Chewing gum (Biotene, Bio-Xtra)

Mouth rinses (Oralbalance, Biotene)

Koppert s LP system?

System based on:- 2 enzymes + 3 substrate components - adjuvant based on a vegetable oil

Based on the formation of reactive oxygen species that inactivate proteins in micro-organisms

Mode of Action LP-system

H2O

OSCN-

OI- HS-R

HOS-RSCN-

I-

LP

H2O2

LACTOPEROXIDASE SYSTEM:

Mode of action

Oxidation of sulfhydryl groups in proteinsMembrane effectsActive transports of amino acids and sugars disturbedGlycolytic enzymes inhibitedOther metabolic functions disturbed

cell death

Activity (1)

Activity based on contactCurative actionActivity not translaminar nor systemic No preventive effectNo residual effectOnly active in water phase High Volume spray not dependent on temperature and relative humidity

Activity (2)

Activity on - fungi - yeast- bacteria- viruses - mycoplasma s

Kills spores, cells, myceliumNo resistance development possible due to multiple-site activity

Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicideLP as a bactericide LP against virusesCompatibility Conclusions

Research

Target 1: powdery mildew(s)

Protected crops worldwide

Research model: cucumber/Sphaerotheca

Composition of active system

Formulation, incl adjuvant

Product developed

KBV 99-01 in cucumber

Control of Powdery Mildew (Sphaerotheca fuliginea ) in cucumber (M. Post, July 2003, Nl)

0

10

20

30

40

50

60

70

80

90

100

Baycor flow KBV 99-01 (1N) KBV 99-01 (2N)

effic

acy

% o

f co

ntr

ol

2 days after treatment 8 days after treatment

Visible effect in cucumber

KBV 99-01 in tomato

Control of Powdery Mildew (Oidium lycopersicum ) in tomato (2002 and 2003, Nl)

0

10

20

30

40

50

60

70

80

90

100

grower 1(Dec '02)

grower 2(May '03)

grower 3(Aug '03)

effic

acy

% o

f co

ntr

ol

Baycor flow KBV 99-01 (1N) KBV 99-01 (2N)

Visible effect in tomato

Research

Target 2: Fusarium oxysporum

Bulbs

Research model: tulip/ zuur

Other bulbs and diseases in research

Introduction to the Lactoperoxidase-systemKoppert s LP-systemHow does LP work?LP as a fungicide LP as a bactericideLP against virusesCompatibility Conclusions

Research

Target: bacterial diseases

Lab trials on Erwinia, Clavibacter, Pseudomonas, Xanthomonas spp. positive

Further research needed

Research

Apple: fire blight

Lab trials positive

Field trial: result moderate

Further research: formulation and application strategy

LP against viruses

Medical uses reported

In vitro trials - TMV - PepMV

LP against viruses

Results:

TMV no effect

PepMV variable results up to 95% effect

More research needed

Patent

Composition and application patented

For use as an agricultural fungicide / bactericide

360DirectNymphs (L1)Orius laevigatius

00

0

0

015

20

56

97

23

22

22

1714

15

Corrected mortality (%)

11

OralContact

WorkersApis mellifera

1ResidueReproductionreduction

1ResidueNymphs (L2-3)

11

ResidueDirect

Nymphs (L2)Macrolophus caliginosus

1ResidueEretmocerus mundus

3ResidueEncarsia formosa

11

ResidueDirectMummies

Aphidius colemani

1ResidueReproductionreduction

1ResidueProtonymphsTyphlodromus pyri

1ResiduePhytoseiulus persimilis

11

ResidueDirectEggs

Amblyseius swirskii

1ResidueAmblyseius cucumeris

IOBCcategory

Direct / residue

Life stageNatural enemy

Compatibility Enzicur with natural enemies

SulphurThiophanate-methyl

Triflumizole

Bicarbonate

Imazalit

Chloorthalovit + prochloraz

Captan

Bitertanol

Azoxystrobin

Fungicides

ThiovitTopsin-M

Mycotal Verticillium lecanii

CalypsoThiacloprid

NomoetTeflubenzaron

OberonSpiromesfen

AdmiralPyriproxifen

RocketAsepta CarexPyridaben

MilstopPlenemPymetrozin

FungaflorPirimozPirimicarb

AllureAdmire Imidacloprid

CaptanTrigardCyromazin

BaycorDipelBacillus thuringiensis

OrtivaVertimecAbamectine

Commercial name

Commercial name

Insecticides

Compatibility Enzicur with pesticides

Advantages of LP system

A unique natural productNo resistance Safe to humans, plants, animalsand the environmentSafe to natural enemies and bumblebeesFits in Integrated Pest Management Fits in organic agriculture

Conclusions

A new type of fungicide and bactericide

New mode of action

Contact activity

Easy to use

Potentially for a broad range of diseases and applications

Contact: [email protected]

Koppert Biological SystemsKoppert takes you further, naturally

Thank you for your attention!

www.koppert.com

[email protected]

a novel, natural anti-microbial product for use as an ... · a novel, natural anti-microbial...

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