a guide to microscopy. the light microscope purpose: history flea glasses anton van leeuwenhoek...
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A Guide to Microscopy
The Light Microscope
Purpose: History
Flea glasses Anton van Leeuwenhoek Robert Hooke
Anton “The Great” Van Leeuwenhoek
Father of microscopy
Apprenticeship Created the first
light microscope
Robert Hooke
First to see cells
Observed cells in cork
The Compound Light Microscope
Uses 2 or more lenses to view a sample Objective lens Ocular lens
Light Source Advantages and Limitations
Parts of Interest
See homework for functions Diaphragm Revolving nose piece Course adjustment knob Fine adjustment knob Which knob to use during high
power?
Magnificaiton
Definition: Eye Piece: _____ Scanning Power: ____ Low Power: ____ High Power: ____ How do you calculate total
magnification?
Resolution
Definition:
Bad Microscope Good Microscope
Limitations of Light Microscope
Poor Magnification Poor Resolution WHY???
Stereomicroscope
Dissecting microscope: Why? Reflects light off of the surface Results in a ___ Image
Electron Microscopes
2 types: Transmission Electron Microscope
(TEM) Scanning Electron Microscope (SEM)
Electrons either pass through a specimen or bounce off of the surface to provide an image
Resolution and Magnification?
Images obtained from EM’s
Electron Microscopes
Advantages? Limitations?