NCM & ANTICANCER

PREPARATION

ELLIPIN

2010-12-27

I.Tsatsral

INTRODUCTION• It is estimated that there will be more than 12 million

new cancer cases in 2007 worldwide, of which 5.4million will occur in economically developed countriesand 6.7million in economically developing countries.

• The corresponding estimates for total cancer deaths in2007 are 7.6 million (about 20,000 cancer deaths aday), 2.9 million in economically developed countriesand 4.7 million in economically developing countries.

• By 2050, the global burden is expected to grow to 27million new cancer cases and 17.5 million cancerdeaths simply due to the growth and aging of thepopulation [1].

INTRODUCTION

• Cancer is the second most cause of death

after cardiovascular diseases in Mongolia.

• General practice of cancer treatments are

either or combination of radiation therapy,

cytostatics and surgery. There are no

natural products available for cancer

treatment in Mongolia [2].

INTRODUCTION

INTRODUCTION

INTRODUCTION• In recent years, there has been increased focus on the

role of specific dietary fatty acids and their effect on health and disease. Polyunsaturated fatty acids (PUFA) have demonstrated a wide range of health-related benefits including improving heart disease related outcomes, decreasing tumour growth and metastasis, and favourably modifying insulin sensitivity.

• Eicosapentaenoic acid (EPA) and docosahexaenoicacid (DHA) from marine sources, in particular, have been studied extensively. In Mongolia the source of PUFA could be livestock, especially cattle liver. Therefore, intent of this review is to outline the technological parameters to obtain fatty acid product containing PUFA, which has an anticancer activity [3].

MATERIALS & METHODS• Cattle liver

• Sodium Chloride

• Chloroform

• Methanol

• Distilled water

MATERIALS & METHODS•Vacuum evaporator

SHD- III

•Variable speed mixer

•Analytical weight

Sartorius

Analytical method

•Free fatty acid content –

Acid value BP 2007

MATERIALS & METHODSEllipin was obtainedthrough stages of x-ray sterilization,fermenting in 0.9%sodium chloridesolution, organicsolvent extraction ofcattle liver. The maincriteria of developingthe processingtechnology wereanticancer activity,yield and free fattyacid content [7].

Preparation of raw

materials

Fermentation

Cooling

Lipid extraction

Distillation

Viscous Substance

RESEARCHInfluence of obtaining method

• The experiment was carried out on 50 rats Kunming. Rats were embedded with EAC-Ehrlich ascites carcinoma cells, and were divided in 5 groups. The control group rats have not been treated. Other 4 groups were injected with physiological solution, ellipin A, B and C, intraperitoneally, in doses 0.3ml/100g.

• The anticancer activity of ellipin, obtained by different technological stages, was determined by intensity of decreasing cancer mass and suppression of cancer cell growth.

RESEARCHInfluence of particle size

• The experiment was carried out on cattle liver,

which was crushed by meat mill and cut to

cube with a size 5 cm.

• The optimal particle size was determined by

yield and free fatty acid content.

RESEARCHInfluence of duration of fermentation

• The experiment was carried out on cattle liver,

which was crushed by meat mill, particle size 7

mm. As the duration of fermentation time has

been selected as 60 hours, 96 hours and 120

hours.

• The optimal duration of fermentation was

determined by yield and free fatty acid content.

RESULTS

N

o

Groups Number of

animals

Cancer

mass, g

Cancer cell

growth

suppression, %Initial End

1 Physiological

solution

9 9 3.8±0.91 -

2 Control 10 10 3.24±1.37 -

3 Ellipin A 9 8 0.95±0.30 70.7

4 Ellipin B 9 9 2.58±1.06 20.4

5 Ellipin C 8 7 2.68±1.34 17.3

RESULTS

0

0.5

1

1.5

2

2.5

3

3.5

4

Cancer mass, g

3.8

3.24

0.95

2.582.68

Physiological solution

Control

Heplipin À

Heplipin Â

Heplipin Ñ

0

10

20

30

40

50

60

70

80

Suppression of cancer growth , %

0% 0%

70.7%

20.4%17.3%

Physiological solution

Control

Heplipin À

Heplipin Â

Heplipin Ñ

RESULTS• According to the survey, Heplipin, which was obtained by

no fermentation with x-ray sterilization, has decreased

cancer mass to 2.58±1.06g as compared to control group

cancer mass of 3.24±1.37g and it has suppressed cancer

cell growth by 20.4%. Heplipin, which was obtained by

fermentation without x-ray sterilization, has decreased

cancer mass to 0.95±0.30g and it has suppressed cancer

cell growth by 70.7%. Heplipin obtained from cattle liver

particle size 5cm, has a yield of 4.04% with free fatty acid

content 48.7%. Heplipin obtained from cattle liver particle

size 7mm, has a yield of 4.57% with free fatty acid

content 52.5%. The duration of fermentation has been

selected as 60 hours, 96 hours and 120 hours and yield

of 2.6%, 3.46%, 4.57% with free fatty acid content

40.82%, 51.77%, 52.51% was obtained, respectively.

RESULTS – PARTICLE SIZE

46

47

48

49

50

51

52

53

Particle size 5cm

Particle size 7mm

Free fatty acid content, %

Free fatty acid content, %

3.73.83.9

44.14.24.34.44.54.64.7

Particle size 5cm

Particle size 7mm

Yield, %

Yield, %

RESULTS - FERMENTATION

DURATION

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

5

60 hours

96 hours

120 hours

Yield, %

Yield, %

0

10

20

30

40

50

60

60 hours

96 hours

120 hours

Free fatty acid content, %

Free fatty acid content, %

CONCLUSION• Results of the study have shown that

fermentation stage has an apparent influence

on anticancer activity than x-ray sterilization

process. The optimal particle size of cattle liver

was found to be 7mm with optimal fermentation

duration of 120 hours.

WORKS CITED1. Global Cancer Facts & Figures 2007, 1

2. National Cancer Center, Cancer records 2007 Mongolia

3. Regulatory activity of polyunsaturated fatty acids in T-cell signaling, Wooki Kim , Naim A. Khan, David N. McMurray, Ian A. Prior f, Naisyin Wang, Robert S. Chapkin , Progress in Lipid Research 49 (2010) 250–261

4. Antitumor efficacy of conjugated Linoleic acid and Lipids (Heplipin, YS2H) from bovine liver, Joint research Institute of Dalian medical University, Dalian 116021, China. (353/305) Cancer Detection and prevention Volume 20 / Issue 5, 1996.

5. Antitumor activities of lipids from cattle liver. K.Hayashi, MD, QW Xu, MD, LF Zhong MD. Okayama 700, Japan, Sino-Japan collaboration institute, Dalian medical University, Dalian 116021, China. (353/305) Cancer detection and prevention. Volume 20/ issue 5, 1996.

6. Õèé, øàð, áàäãàí - ÌÁ øèíý ñàíàà áà õàâäðûí ýñðýã ýìèéíñóäàлãàà, Ì.Àìáàãà, Ë.Õ¿ðýлáààòàð, Ê.Õàÿàøè, Á.Ñàðàíöýöýã, “Õîðòõàâäàð” ñýòã¿¿л 1999 ¹ 2

7. Г.Эðäýíýíöýöýã, Ñ.Õàíäñ¿ðýí, Ш.Эíõзàÿà Эìèéí òåõíîлîãè II áîòь 257-260 õ, 2005 îí, Улàíáààòàð õîò