2019-04-16- liste des vecteurs et comparatif transfection · 4/16/2019 · mediated by the mannose...
TRANSCRIPT
SynNanoVect – lipofection
2019
https://www.univ-brest.fr/synnanovect/1/12
2/12
�Amphiphiles & Transfection
LiposomesCationic
lipid
Nucleic acid
lipoplexesTransfection
in vitro or in vivo
Bio
lum
ine
sc
en
ce
(ph
pix
-1se
c-1
Ctrl+ TestCtrl-
Lipofection
�Simple protocol to transfect a wide variety of cells
�High transfection efficacies and low toxicity
3/12
�Transfection of progenitor HepaRG at day 1
New tools
Intensity of
fluorescence74 %
64 %
40 %
Comparison of transfection efficacies and toxicity with two other commercial kits
Side-effectsMTT assay
SNV-1 Optimized for the transfection of HepaRG cell line
% of
transfected cells
SNV-1
SNV-1
SNV-1
SNV-1
4/12
SNV-2 Optimized for transfection of HeLa, A549, 16HBE, SKMel28, C2C12
EfficacyIntensity of
bioluminescence
Side-effectsMTT assay
(% cell viability)
New tools SNV-2
Dual electroporation systems For cells hard to transfect by lipofection
Electroporation
MICROPORATOR Neon™ system
NB : Core Unit , X Unit (cuvettes), Y Unit (24 well-platew) &
Shuttle modulepour for 96 well-plates
Invitrogen-Life Sciences®
AMAXA/LONZA®
NUCLEOFECTOR 4D
Cells in suspension
Cells in suspension and adhrent cells
5/12
6/12
1
� Bioluminescence imaging � Biofluorescence imaging
In vivo applications
7/12
1
SNV-1 Optimized for the transfection of HepaRG cell line
SNV-2 Optimized for the transfection of HeLa, A549, 16HBE, SKMel28, C2C12
1 mL (≈ 100 transfections - 2.5x105 cells each time)
562 euros HT
Contact : [email protected]
1 mL (≈ 100 transfections - 2.5x105 cells each time)
562 euros HT
Contact : [email protected]
PRICE
SynNanoVect – Trimannosylated lipid
as a targeting lipid
2019
https://www.univ-brest.fr/synnanovect/8/12
9/12
�Tri-Man-Lip100 for trimannosylated liposomes, lipoplexes
(LR) and lipopolyplexes (LPR)
Dendritic cell targeting lipid
O
OHO
HO
OH
O
2
O
OHO
HO
OH
OH
O
2
O
OHO
HO
OH
OH
O
2
O
O
NH
O
OO
HN
O5
OH
Tri-Man-Lip100
10/12
�Liposomes, Lipoplexes and lipopolyplexes incorporating Tri-Man-Lip100 induce strong binding on DC cells
mediated by the mannose receptor
Dendritic cell targeting lipid
�Lipopolyplexes with Tri-Man-Lip100 combine strong
antitumor T Cell immunity with improved inflammatory safety
ACS Nano, 2018, 12, 9815-9829
Carbohydr. Res., 2016, 435, 142-148
J. Control. Release, 2018, 278, 110-121
�Lipopolyplexes with Tri-Man-Lip100 give rise to an efficient stimulatory immune response allowing anti-cancer
vaccination in mice
11/12
1Optimized for the targeting of dendritic cells
1 mg 678,5 euros HT
Contact : [email protected]
PRICE
Tri-Man-Lip100
12/12
�SynNanoVect Platform
J. Med. Chem. 2010, 53, 1496-1508 ; Biotechnology J., 2010, 5, 314-320 ; Org. Biomol. Chem., 2011, 9, 2422-2432 ; J.
Drug Targeting, 2011, 19, 315-325 ; Nanomedicine: NBM 2011, 7, 445-453 ; Eur. J. Org. Chem., 2011, 6294-6303 ; Biochimie, 2012, 94, 33-41 ; Int. J. Pharm.., 2012, 423, 144-150 ; Biomaterials, 2012, 33, 2980-2990 ; Org. Biomol.
Chem., 2012, 10, 2051-2058 ; Biomaterials, 2012, 33, 6240-6253 ; Biophys. Chem., 2013, 171, 46-53 ; Adv. Healthcare
Mater, 2013, 2, 1513-1524 ; Org. Biomol. Chem., 2013, 11, 1650-1658 ; Eur. J. Org. Chem., 2014, 1074-1083 ; Int. J.
Pharm., 2014, 460, 1-2, 264-272 ; Methods Mol. Biol., 2013, 987, 295-314 ; Org. Biomol. Chem., 2014, 12, 1463-1474 ;
Mol. Pharm., 2014, 11, 2973-88. ; Eur. J. Org. Chem., 2014, 8041-8048 ; Org. Biomol. Chem., 2015, 13, 1122-1132 ; Mol. Pharm., 2015, 12, 1902-1910 ; Biotechnol. J., 2015, 10, 1370–1389 ; Colloids and Surfaces B: Biointerfaces, 2015, 136,
192-200 ; Nanomedicine: NBM, 2015, 11, 1735–1744 ; Biomaterials, 2015, 59, 1-11 ; Inter. J. Mol. Sci., 2015, 16,
26055–26076 ; J. Gene Med, 2016, 18, 3-15 ; Org. Biomol. Chem., 2016, 14, 2846-53 ; Org. Biomol. Chem., 2016, 14,
3744-3751 ; Soft Matter, 2016, 12, 4516 – 4520 ; Int. J. Pharm.., 2017, 524, 268-278 ; J. Mater. Chem. B, 2017, 5, 6869-6881 ; Inter. J. Pharm., 2018, 536, 29-41 ; J. Control. Release, 2018, 278, 110-121 ; ACS Nano, 2018, 12, 9815-9829 ;
� Services, expertise and tools for transfection or vectorisation
� 4 research groups from Brittany (Brest & Rennes)
� Member of the Biogenouest platform network
� Awarded IBISA since 2008
� ISO 9001 since 2013
� Our publications in the field of lipofection since 2010 (selection)
Transfection & Research