2017 cap press catalog folders...2017 cap press catalog ... table of contents benchtop reference...

2017 CAP Press Catalog

Publications

Introducing five new titles and three revised editions

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All CAP Press books are written and edited by recognized experts in pathology and laboratory medicine. Find publications on a variety of topics:

• Clinical pathology

• Anatomic pathology

• Quality management

• Practice management

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© 2017 College of American Pathologists. All rights reserved. 25293.0117

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Table of Contents

Benchtop Reference Guides

Gram Stain Benchtop Reference Guide NEW .................................................................................................…… 2

Arthropod Benchtop Reference Guide .................................................................................................................. 2

Parasitology Benchtop Reference Guide ................................................................................................................ 2

Mycology Benchtop Reference Guide ..................................................................................................................... 2

Urinalysis Benchtop Reference Guide .................................................................................................................... 3

Body Fluids Benchtop Reference Guide .................................................................................................................. 3

Hematology Benchtop Reference Guide ................................................................................................................. 3

Clinical Pathology

Transfusion Medicine in the Hot Seat NEW .......................................................................................................... 4An Algorithmic Approach to Hemostasis Testing, 2nd edition NEW EDITION ..................................................... 4

So You’re Going to Collect a Blood Specimen, 15th edition NEW EDITION .......................................................... 4

Flow Cytometry in Evaluation of Hematopoietic Neoplasms ................................................................................... 5

Clinical Toxicology Testing ...................................................................................................................................... 5

Point-of-Care Testing Toolkit ................................................................................................................................. 5

Color Atlas of the Urinary Sediment ....................................................................................................................... 6

Color Atlas of Body Fluids ...................................................................................................................................... 6

Color Atlas of Hematology ...................................................................................................................................... 6

Special Interest

The Healing Art of Pathology NEW ........................................................................................................................ 7

Atlas of Paleopathology ......................................................................................................................................... 7

The Best of CAP TODAY Q&A ................................................................................................................................... 7

Quality and Laboratory Management

Quality Management in Anatomic Pathology NEW ............................................................................................... 8

Patient Safety in Anatomic & Clinical Pathology Laboratories NEW .............................................................. …… 8

Laboratory Administration for Pathologists ............................................................................................................ 9

Developing and Organizing an Institutional Biospecimen Repository...................................................................... 9

Quality Management in Clinical Laboratories ......................................................................................................... 9

Anatomic Pathology

Autopsy Performance & Reporting, 3rd edition NEW EDITION .......................................................................... 10

An Introduction to Autopsy Technique, 2nd edition ............................................................................................... 11

Special Autopsy Dissections ................................................................................................................................ 11

Autopsy: Aiding the Living by Understanding Death (brochure) ............................................................................ 11

Atlas of Transplant Pathology .............................................................................................................................. 12

Histologic Preparations ........................................................................................................................................ 12

CAP Practical Guide to Gynecologic Cytopathology .............................................................................................. 12

Basic Competencies in Forensic Pathology .......................................................................................................... 13

Handbook of Forensic Pathology, 2nd edition ....................................................................................................... 13

Cause of Death and the Death Certificate ............................................................................................................. 13

Print Publications Ordering

Ordering Information and Publications List .......................................................................................................... 14

Print Publications Order Form .............................................................................................................................. 15

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Mycology Benchtop Reference Guide is an illustrated guide to common fungi. Full-color colony and microscopic views are featured. The content includes detailed descriptions of the morphology, ecology, and clinical significance, with more than 70 identifications of fungi commonly encountered in the laboratory, including yeast and yeast-like fungi, molds with narrow hyaline hyphae, dimorphic fungi, dermatophytes, zygomycetes, and dematiaceous molds.

Mycology Benchtop Reference Guide An Illustrated Guide for Commonly Encountered FungiGordon L. Love, MD, D(ABMM), FCAP

Spiral bound; 92 pages;

70+ images; 2013

Item number: MBRGList price: $89

Ebook price: $60


Arthropod Benchtop Reference Guide An Illustrated Guide for Commonly Encountered Arthropods Blaine A. Mathison, BS, M(ASCP); Bobbi S. Pritt, MD, MSc, (D)TMH, FCAP

Arthropod Benchtop Reference Guide is an illustrated guide to common arthropods. The content includes detailed descriptions of the arthropod morphology, ecology, and clinical significance, with more than 25 identifications of ectoparasites including ticks, mites, lice, bed bugs, kissing bugs, fleas, myiasis-causing fly larvae, and pseudoparasites.


65+ images and tables; 2016

Item number: : ABRGList price: $89

Ebook price: $60

Gram Stain Benchtop Reference Guide is an illustrated guide to Gram stain preparation and gram-positive, gram-negative, and other microorganisms that can be identified with the Gram stain. The content includes background information on the Gram stain, including theory, specimen preparation, and biosafety; microbial morphology, quantitation of microorganisms, and indicators of pathology; and numerous identifications of organisms found in blood, urine, CSF, body fluids, tissue, respiratory, and genital specimens.

Gram Stain Benchtop Reference Guide An Illustrated Guide to Microorganisms and Pathology Encountered in Gram-Stained Smears D. Jane Hata, PhD, D(ABMM); Richard B. Thomson Jr., PhD, D(ABMM)

Spiral bound; 100 pages; 115+ images and tables; 2017

Item number: GSBRGList price: $89 Ebook price: $65

Parasitology Benchtop Reference Guide An Illustrated Guide for Commonly Encountered ParasitesBobbi S. Pritt, MD, MSc, (D)TMH, FCAP

Parasitology Benchtop Reference Guide is an illustrated guide to common parasites. The content includes detailed descriptions of the parasite morphology, ecology, and clinical significance, with more than 70 identifications of parasites commonly found in the clinical laboratory, including blood parasites, intestinal protozoa, helminthes, macroscopic worms, and miscellaneous specimens.

Spiral bound; 98 pages; 70+ images and tables; 2014

Item number: PBRGList price: $89Ebook price: $60

New

800-323-4040 | 847-832-7000 option 1 (Country code 001) 3

Urinalysis Benchtop Reference Guide An Illustrated Guide for Cell MorphologyJoan E. Etzell, MD, FCAP; Kyle T. Bradley, MD, MS, FCAP; David F. Keren, MD, FCAP; Sherrie L. Perkins, MD, PhD, FCAP; Janet Piscitelli, MD, FCAP; Anna K. Wong, MD, FCAP; George M. Girgis, MT

Spiral bound; 42 pages; 36 images; 2013

Item number: BFBRGList price: $89Ebook price: $50

Body Fluids Benchtop Reference Guide is an illustrated guide to common and rare cells. The content includes detailed descriptions of each cell, including facts, cell morphology, and inclusions, with 36 color images demonstrating common and rare cells (erythroid, lymphoid, myeloid, and mononuclear phagocytic series; lining cells; miscellaneous cells), crystals, microorganisms, and miscellaneous findings.

Body Fluids Benchtop Reference Guide An Illustrated Guide for Cell MorphologyJoan E. Etzell, MD, FCAP; Martha R. Clarke, MD, FCAP; George Girgis, MT(ASCP); Alice L. Werner, MD, FCAP; Anna K. Wong, MD, FCAP; Tracy I. George, MD, FCAP

Hematology Benchtop Reference Guide is an illustrated guide to common and rare cells. The contents include detailed descriptions for each cell morphology, with more than 50 different cell identifications, including both common and rare erythrocytic cells and inclusions, granulocytic (myeloid) and monocytic cells, lymphocytic cells, platelets and megakaryocytic cells, microorganisms, and artifacts.

Hematology Benchtop Reference Guide An Illustrated Guide for Cell MorphologyCAP Hematology and Clinical Microscopy Committee Spiral bound;

60 pages; 50+ images; 2012

Item number: HBRGList price: $89

Ebook price: $50

Urinalysis Benchtop Reference Guide is an illustrated guide to entities found in the urine. The content includes detailed descriptions for each morphology, with more than 30 color images, including common and rare cells, casts, crystals (classified by pH), organisms, and miscellaneous/exogenous findings.

Parasitology Benchtop Reference Guide An Illustrated Guide for Commonly Encountered ParasitesBobbi S. Pritt, MD, MSc, (D)TMH, FCAP


34 images; 2014

Item number: UABRGList price: $89

Ebook price: $50


CAP Benchtop Reference GuidesThese sturdy laminated guides feature tabbed sections for easy reference. For students and medical technologists, they are an affordable way to identify commonly encountered entities quickly and confidently.

Two unique sizes: 6½” x 7” (Gram Stain, Arthropod, Parasitology, Mycology) 5” x 6½” (Urinalysis, Body Fluids, Hematology)


So You’re Going to Collect a Blood Specimen is a basic instructional text and functional reference guide for phlebotomy. This well-illustrated manual provides step-by-step instruction for obtaining blood by venipuncture and skin puncture from adult and pediatric patients. Blood smears, blood cultures, and neonatal screening procedures are also included, as is the order of draw for different procedures, with the goal of obtaining a quality specimen and avoiding specimen rejection.

Both routine collections and those with special circumstances are covered. Safety, including standard precautions, sterility, competency assessment, and quality assurance, is emphasized throughout.

So You’re Going to Collect a Blood Specimen An Introduction to Phlebotomy, 15th editionFrederick L. Kiechle, MD, PhD, FCAP, editor

Transfusion Medicine in the Hot Seat is a useful text for pathology trainees and an educational resource for pathologists who cover transfusion medicine. The text is divided into three sections: antibodies, blood components, and complications. Each section presents numerous short cases that illustrate real-world transfusion scenarios, with 26 cases total. The short-case format makes the information easily accessible and can serve as the basis for a transfusion medicine curriculum in clinical pathology.

The content includes blood, platelet, and cryoprecipitate transfusions as well as hematopoietic stem cell transplantation.

Transfusion Medicine in the Hot Seat A Case-Based Primer Karen Quillen, MD

Softcover; 123 pages; 2016 ISBN: 978-098370687-8

Item number: PUB224List price: $90Member price: $72Ebook price: $63

Clinical Pathology

An Algorithmic Approach to Hemostasis Testing is an up-to-date, well-illustrated reference text and practical guide for pathologists and laboratories engaged in hemostasis testing. It includes sections on hemostasis physiology, the basics of laboratory testing and interpretation, specimen collection and processing, patient history and diagnostic criteria, bleeding disorders, thrombophilic disorders, and antiplatelet and anticoagulant drug monitoring. Throughout the text are numerous testing algorithms to assist in differential diagnoses.

This edition includes new chapters on quality, emergency testing, consultation, thrombotic microangiopathies, and new antifibrinolytic, thrombolytic, and anticoagulant drugs.

An Algorithmic Approach to Hemostasis Testing, 2nd editionKandice Kottke-Marchant, MD, PhD, FCAP, editor

Hardcover; 480 pages; 175+ figures, tables,

and algorithms; 2016ISBN: 978-194109625-3

Item number: PUB223List price: $185

CAP member price: $148Ebook price: $120

Spiral bound; 84 pages; 30+ images and tables; 2017 ISBN: 978-194109634-5

Item number: PUB225List price: $35CAP member price: $28Ebook price: $25

Volume pricing available

New Edition

New

New Edition


Clinical Pathology

Flow Cytometry in Evaluation of Hematopoietic Neoplasms A Case-Based Approach Sindhu Cherian, MD, FCAP; Brent Wood, MD, PhD

Hardcover; 176 pages; 90+ figures comprising hundreds

of dot plots; 2012 ISBN: 978-098370682-3

Item number: PUB221 List price: $110

Member price: $88Ebook price: $82

Clinical Toxicology TestingA Guide for Laboratory ProfessionalsBarbarajean Magnani, PhD, MD, FCAP; Michael G. Bissell, MD, PhD, MPH, FCAP; Tai C. Kwong, PhD, DABCC, FACB; Alan H.B. Wu, PhD, DABCC, FACB, editors

Clinical Toxicology Testing is a practical guide to setting up, overseeing, and directing hospital toxicology laboratory operations. The book focuses on the complex issues facing the laboratory director or pathologist in this role, as well as those in training in the field of laboratory medicine. It addresses not only pharmacological principles, testing menus, and methodologies, but also clinical test interpretation for specialized areas such as the emergency department, the pain clinic, and the autopsy suite. In addition, it serves as an introduction to such topics as workplace drug testing and relevant regulatory issues.

Contents include organizational aspects of a hospital toxicology laboratory, forensic toxicology and chain of custody, adverse drug events and poisonings, analytical methodologies and alternate specimens for testing, therapeutic drug monitoring and compliance testing, pharmacokinetic concepts and pharmacogenomic testing, and drugs of abuse, toxic substances, and overdoses.

Softcover; 304 130+ figures and tables; 2012ISBN: 978-098370681-6


Point-of-Care Testing Toolkit is a resource for any member of the POCT team who wants to learn about POCT or who has responsibility to guide or direct POCT. Contents include POCT advantages and disadvantages; current and projected technology; pathologist, laboratory director, and point-of-care coordinator roles; selection of appropriate test methods; validation and verification protocols; quality control and data management; and POCT training and competency.

Point-of-Care Testing ToolkitCAP Point-of-Care Testing (POCT) Resource Committee

Ebook only;

97 pages, 2015eISBN:

978-194109624-6Ebook price: $25

Flow Cytometry in Evaluation of Hematopoietic Neoplasms is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. The text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing).

Contents include an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem-cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion.


Clinical Pathology

Color Atlas of Hematology An Illustrated Field Guide Based on Proficiency TestingEric F. Glassy, MD, FCAP, editor

A classic text representing years of hematology survey data, Color Atlas of Hematology lists the critical keys to successful morphologic identification of cells, ranging from the routine to the markedly abnormal. Detailed “A Closer Look At...” sections offer in-depth discussion of clinical information, pathophysiology, and identification details on various topics.

Contents include granulocytic (myeloid) cells, erythrocytic cells and inclusions, nucleated red cells, megakaryocytic cells and thrombocytes, lymphocytic and plasmacytic cells, microorganisms, artifacts, and miscellaneous cells.

Hardcover; 380 pages; 400+ photomicrographs, illustrations, and tables; 1998ISBN: 978-093030466-9

Item number: PUB210List price: $125, Member price: $100

Color Atlas of the Urinary SedimentAn Illustrated Field Guide Based on Proficiency Testing Meryl H. Haber, MD, FCAP; David Blomberg, MD, FCAP; Katherine Galagan, MD, FCAP; Eric F. Glassy, MD, FCAP; Patrick C.J. Ward, MB BCh, editors

Color Atlas of the Urinary Sediment includes photo-micrographs of the urinary sediment, a discussion of the pertinent morphologic features of each element, and the results of the identification of the particular element by both referees and participants in the CAP’s proficiency testing program. Additional images not used in CAP Surveys are also included.

Contents include renal anatomy and physiology, urinalysis in health and disease, cells, casts, crystals, organisms, and miscellaneous findings.

Color Atlas of Body FluidsAn Illustrated Field Guide Based on Proficiency TestingKatherine A. Galagan, MD, FCAP; David Blomberg, MD, FCAP; P. Joanne Cornbleet, MD, PhD, FCAP; Eric F. Glassy, MD, FCAP, editors

Color Atlas of Body Fluids is a comprehensive reference based on more than 20 years of proficiency testing in body fluids. Along with an overview of cytology and technical considerations, contents include the anatomy and formation as well as the collection and preparation of cerebrospinal fluid, synovial fluid, and pleural, peritoneal, and pericardial fluids.

The text contains numerous photographic examples along with brief write-ups to highlight the most important features of various cell types, including erythroid series, lymphoid series, myeloid series, mononuclear-phagocytic series, lining cells, and miscellaneous cells.

Hardcover; 370 pages; 650+

photomicrographs, figures, and

tables; 2006ISBN: 0-93030491-8

Item number:

PUB216List price: $150

Member price: $120

Hardcover; 464 pages; 800+photomicrographs, figures, and tables; 2010 ISBN: 978-093030487-4

Item number: PUB219List price: $175 Member price: $140


The Best of CAP TODAY Q&AFrederick L. Kiechle, MD, PhD, FCAP, editor

Atlas of Paleopathology documents 40 years’ experience in excavating and examining mummified remains in South America. The numerous images presented represent a multidisciplinary study comprising pathologists, anthropologists, and archaeologists investigating pre-Colombian civilizations from the regions that are today known as Peru and Chile.

Contents include mummies and artifacts, diseases of the skeletal system, diseases of soft tissues, tumors, intentional cranial deformation, and paleoneuropathology and trephination.

Atlas of Paleopathology Autopsies in South American MummiesEnrique Gerszten, MD, FCAP; Marvin J. Allison, PhD; Brianna E. Maguire, BS; Peter C. Gerszten, MD

Special Interest

The Healing Art of PathologyMarilyn M. Bui, MD, PhD, FCAP; Katherine A. Galagan, MD, FCAP, editors

The Healing Art of Pathology focuses on the discipline of pathology as seen through the lens of art. The artwork and stories presented celebrate the courage of patients, the compassion of physicians, and the strength of the human spirit. Various media are represented, including paint, fabric, ceramic, glass, digital images, poetry, and prose.

Contents include the role of the pathologist in patient care, art by patients and their families, art by pathologists, art in collaboration with pathologists, art by health care professionals and their families, and Art in Healthcare (Scotland).

Softcover; 94 pages; 2016

ISBN: 978-098370688-5

Item: PUB315List price: $50

Member price: $40

Softcover; 140 pages; 120+ images; 2012 ISBN: 978-098370683-0

Item number: PUB313List price: $35Member price: $28


ISBN: 978-093030499-7


Member price: $24

The Best of CAP TODAY Q&A is a compilation of experts’ answers to readers’ questions as published in the popular “Q&A” section of CAP TODAY. Contents include body fluids, hematology and coagulation, chemistry, microbiology, cytology, molecular diagnostics, point-of-care testing and phlebotomy, specimen processing, critical values and stat tests, transfusion medicine, and toxicology/therapeutic drug monitoring.

New

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Patient Safety in Anatomic & Clinical Pathology LaboratoriesDeborah Sesok-Pizzini, MD, MBA, FCAP, editor

Patient Safety in Anatomic & Clinical Laboratories describes patient safety culture in the laboratory and how it fits in with the larger mission and purposes of the health care enterprise. The text addresses common types of errors seen in pathology and the role of cognitive bias in contributing to these errors, reducing errors through communication and technology, and tools and methods to improve patient safety. Also addressed are building high-reliability teams and the role of the patient navigator in addressing patient safety issues through continuity, coordination, and care. The book also describes developing and implementing a patient safety curriculum in order to fulfill ACGME training requirements to meet standards for resident and fellow education for anatomic pathology and laboratory medicine.

Softcover; 128 pages;2017

ISBN: 978- 194109636-9

Item number: PUB316

List price: $110 Member price: $88

Ebook price: $80

8

Quality Management in Anatomic Pathology Strategies for Assessment, Improvement, and AssuranceQihui “Jim” Zhai, MD, FCAP; Gene P. Siegal, MD, PhD, FCAP, editors

Quality Management in Anatomic Pathology is a comprehensive manual designed to improve patient care while ensuring your laboratory achieves it accreditation standards. It provides pathologists and laboratory directors with the tools necessary to develop, implement, and maintain a comprehensive quality program, with an emphasis on regulatory compliance and cross-references to the CAP Laboratory Accreditation Program checklists and CLIA.

Contents include:

• General approaches to quality management, including designing a quality management plan; regulatory compliance; defining, classifying, and responding to errors; error prevention; and quality improvement plan components and monitors

• Systematic approaches to quality management in the histology laboratory, intraoperative consultation, cytopathology, autopsy pathology, immunohistochemistry, and molecular diagnostics

• Critical components in contemporary anatomic pathology, including informatics, ISO 15189, and specimen tracking systems

• Minimizing damage by actively responding to serious diagnostic errors

• Legal and regulatory issues surrounding telepathology and molecular pathology

The text also contains numerous tables and checklists that can be adapted for use by individual laboratories.


Softcover; 228 pages; 135+ figures and tables; 2017ISBN: 978-194109629-1

Item number: PUB125List price: $115 Member price: $92Ebook price: $81

New

New

800-323-4040 | 847-832-7000 option 1 (Country code 001)

Hardcover; 300 pages; 100+ figures, tables, and

photographs; 2011ISBN: 978-098370680-9



Laboratory Administration for PathologistsElizabeth A. Wagar, MD, FCAP; Richard E. Horowitz, MD, FCAP; Gene P. Siegal, MD, PhD, FCAP, editors

Laboratory Administration for Pathologists provides the core principles and tools of management for the practice of pathology and laboratory medicine. The book covers all aspects of clinical and anatomic pathology laboratory administration, and is especially geared for trainees and young pathologists entering the field. Pathologists will find this text to be useful as a quick reference tool or to review management for maintenance of certification. Residents will find this text helpful for board preparation and in rotations related to management training.

Contents include management principles, laboratory operations, personnel management, finding and evaluating a pathology position, equipment and supplies, laboratory information systems, quality management, financial management of the laboratory and the pathology practice, regulations, legal affairs, and ethics.

Developing and Organizing an Institutional Biospecimen Repository provides useful information on establishing and maintaining a biospecimen repository. It discusses issues critical to specimen collection, processing, storage, and distribution. Contents include defining the role of the biospecimen repository, developing an institutional resource within the pathology department, management and key personnel, infrastructure of the repository and associated core laboratories, flow of biospecimens to and from the repository, quality control, informatics, informed consent, cost assessment for developing a biorepository, and relationships with outside institutions and industry.

Developing and Organizing an Institutional Biospecimen RepositoryDonna E. Hansel, MD, PhD; Scott D. Jewell, PhD, editors

Softcover; 118 pages; 2014ISBN: 978- 098370685-4


9


Quality Management in Clinical Laboratories Promoting Patient Safety Through Risk Reduction and Continuous Improvement Paul N. Valenstein, MD, FCAP, editor

Quality Management In Clinical Laboratories is a practical “how-to” manual written for the laboratory director, supervisor, and practicing pathologist. The manual is designed to help readers manage quality and patient safety in clinical laboratories, comply with quality and patient safety regulations and accreditation requirements, and develop and administer a quality management plan. The text covers the most important standards and areas that have proven to be particularly problematic in the management of clinical laboratories. Patient safety issues— an essential and inseparable component of laboratory quality— are discussed throughout the text.

Contents include case studies based on knowledge of actual events; approaches to managing quality and patient safety; regulation and accreditation; specific quality and patient safety risks and control measures for preanalytic, analytic, postanalytic, and general laboratory operations; and the laboratory quality management plan.

Softcover; 265 pages; 80+ exhibits and

tables; 2005ISBN: 0-93030488-8



Quality ManagementIn Clinical Laboratories

Promoting Patient Safety Through Risk Reduction And

Continuous Improvement

Paul Valenstein, MDEditor


Hardcover; 472 pages; 1,000+ images and tables; 2017 ISBN: 978-194109632-1

Item number: PUB126List Price: $160Member price: $128Ebook price: $112

Anatomic Pathology

Autopsy Performance & Reporting, 3rd editionKim A. Collins, MD, FCAP, editor

Autopsy Performance & Reporting is a comprehensive, highly-illustrated text organized into seven sections: introduction to the autopsy, preautopsy and administration, autopsy safety, autopsy performance, special studies, autopsy reporting, and quality assurance. Also covered are specialized autopsies, procedures for specific organs and anatomic systems, cause of death, and standardized reporting formats.

This newly updated edition is for seasoned autopsy pathologists and those who may not deal with autopsies on a regular basis. Contents include:

• Autopsy overview, history, moral obligations, and cultural and religious beliefs

• CAP policies and documents pertaining to the autopsy

• Autopsy in medical education

• Nonforensic regional autopsy centers, autopsy facility design, and decedent affairs programs

• High-risk autopsy cases and precautions for autopsies of patients with Creutzfeldt-Jakob disease

• Maternal, perinatal, and pediatric autopsy

• Examination of the placenta

• Special studies of the heart and lungs

• Procedures for the brain, spinal cord, and neuromuscular system

• Alzheimer’s disease and other dementias

• Embalmed or exhumed remains

• Forensic pathology

• Organ and tissue procurement, and evaluation of transplant recipients

• Implanted medical devices

• Ancillary studies, including microbiologic testing

• Photography and photomicrography

• Postmortem radiology and imaging

• Autopsy lexicon and medical certification of death

• Quality management, the CAP Laboratory Accreditation Program, and accreditation

to CAP 15189

V | Special StudieS ancillary StudieS | 28

130 131

usually considered contaminated unless the clinical his-tory suggests otherwise.2 Correlation of the clinical his-tory with culture results is of paramount importance when practicing postmortem microbiology. In general, routine cultures in cases where infection is not clinically indicated may be a waste of time and resources. If infec-tion is in the clinical differential, however, positive cul-tures in two or more sites are of great significance. For this reason, in cases of possible or suspected infection, blood samples should be accompanied by solid organ cul-tures (eg, spleen, lung, liver) and biopsies of any lesions. See also chapter 29, Postmortem Microbiological Testing.

Obtaining the SpecimenSolid tissue specimens are obtained by sterilizing the surface of the organ with a red hot spatula and using sterile needles (for fluids) or blades (for tissues).1,2,5 Some researchers have advocated using iodophors or other an-timicrobial agents to sterilize the surface instead of the hot spatula.2 Sterile instruments should be used for each specimen. A sterilely excised, 1-cm3 tissue block is opti-mal for homogenation and most closely represents the in vivo infection status of the organ.4

Blood samples are usually taken from the sterilized surface of the right atrium or inferior vena cava and

transferred immediately to culture medium. Right versus left heart blood has been studied but to date is inconclu-sive as to preference.6 Before the autopsy, adequate blood samples may also be obtained from the subclavian area (after thorough sterilization of the skin).5 This can be done promptly even if the autopsy is delayed.5

Cerebrospinal fluid may be obtained by either a lum-bar puncture, cisterna magnum puncture (below the oc-ciput through the atlanto-occipital membrane into the basal cistern), or from the lateral ventricles of the brain after the cranial vault has been removed.7 The fluid can be Gram stained and cultured (Figures 28-1 through 28-3).

TransportSeparate aerobic and anaerobic media are used for the transport of blood. The aerobic bottle may be held for fungal cultures if indicated. Aerobic and anaerobic media are also available for tissue samples. Make sure that the tissue is pushed into the medium of the anaerobic trans-port in order to maintain an anaerobic environment. A special culture broth is used for mycobacterium.

Sterile sections of tissue or a sterile swab from a lesion or exudate may be submitted for viral culture. A trans-port medium must be obtained and then placed on ice for transport. DNA probes have been used on tissue sec-tions to look for viruses such as cytomegalovirus (CMV). Human immunodeficiency virus (HIV) antibodies, hep-atitis C antibodies, and hepatitis B surface antigen can be sought by submitting blood in a red or marbled-red top (serum separator) tube.

A “culturette” containing two sterile swabs may be used for swabbing lesions, abscess contents, effusions, or exudates (Figure 28-4). This particular medium will de-tect both aerobic bacteria and fungi. Also, swabs of the nasopharynx may be placed in viral transport medium for analysis.

Complementary StudiesThe following can be performed on tissue sections: (1) Gram stain/Brown-Brenn stain for bacteria (Figure 28-

5); (2) acid-fast stain and auramine-rhodamine fluores-cent stain for mycobacterium (Figure 28-6); (3) acid-fast stain for Nocardia; (4) periodic acid–Schiff (PAS) stain for fungus; and (5) Grocott-Gomori methenamine sil-ver nitrate (GMS) stain for fungus. Immunofluorescence staining can also be used on tissue sections to look for microorganisms such as Neisseria or Haemophilus spe-cies. Special Gen-Probe swabs may be used for detection of certain organisms, such as Chlamydia trachomatis and Neisseria gonorrhoeae.

HistochemistryIn additional to routine histologic sections with hema-toxylin-eosin (H&E) stain, “special stains” with or with-out polarization may be required on tissue sections ob-tained at autopsy (Figures 28-7 through 28-9). The vast majority of these stains can be performed on tissues fixed in 10% buffered formalin. Table 28-2 lists some common uses of histochemical special stains on autopsy material.

Immunohistochemistry and ImmunofluorescenceAlthough little has been written about postmortem im-munohistochemistry and immunofluorescence, anecdot-al evidence suggests that immunostains can play a useful role in characterizing poorly differentiated malignant neoplasms discovered at autopsy (Figures 28-18 through

Figure 28-1. Cisternal puncture for procurement of cerebrospinal fluid. Images courtesy of Katherine Krupela, BA, and Eimad Zakariya, MD, JD.

Figure 28-2. An alternative way to draw cerebrospinal fluid (CSF) postmortem is through the lateral cerebral ventricle. The CSF can be used for microbiology, toxicology screens using dip-sticks, and other ancillary studies. Image courtesy of Adrienne Segovia, MD.

Figure 28-3. Aspiration of cerebrospinal fluid by gently sepa-rating the cerebral hemispheres and entering the cerebral ven-tricles with a sterile needle attached to a sterile syringe. Note the Normal gross appearance of clear, colorless cerebrospinal fluid. Image courtesy of Katherine Krupela, BA, and Eimad Zakariya, MD, JD.

Figure 28-4. Culturette for swabbing exudates, abscesses, or-gans, and lesions for culture.

Figure 28-5. Liver with postmortem overgrowth of bacteria. Gram stain. Image courtesy of Mary Ann Sens, MD, PhD.

Figure 28-6. Acid-fast stain positive for Mycobacterium tubercu-losis. Image courtesy of Richard Conran, MD, JD.

Figure 28-7. Routine hematoxylin-eosin staining can differen-tiate premortem thrombus or thromboemboli (left) from post-mortem blood clots (right).

Figure 28-8. Top: Lung (left, hematoxylin-eosin; right, elastic Van Gieson stain). Bottom: Lung with polarization.

28-21). One study examined lymph nodes (both reactive and those containing metastatic carcinoma) allowed to autolyse in vitro during a 72-hour period.10 This study showed that LCA, UCHL-1, AE1, and EMA were the

IV | Autopsy performAnce

152

A

Figure 18-2. A. Normal membrane attachment to the disc may be at the margin, as shown in the inferior half of this placenta, or circummarginate, as shown in the superior half of this placenta. B. Circumvallate membrane attachment is considered pathologic only if it involves the entire membrane attachment site. The chorionic plate is folded back on itself forming a distinct ridge, often several centimeters away from the actual margin. This placenta also has a large chorionic cyst filled with yellow fluid.

B

A

Figure 18-3. A. At term, meconium-stained membranes are a common finding. Thick, viscid meconium may be problematic from the standpoint of meconium aspiration syndrome or the underlying stressor that causes meconium to be passed. Initially, the me-conium is dark green but with time will become browner to yellow. B. Meconium will cause vacuolization and hyperplasia of the amnion epithelium and be taken up by macrophages in the amnion in approximately 1 hour. C. Meconium will be taken into the chorion within 3 hours and into the decidua approximately 6 hours from the time of passage to delivery.

B C

A

Figure 18-4. Amnion nodosum is evidence of severe and prolonged oligohydramnios. A. The small yellow-white plaques will be present on all amnion surfaces and can be scraped off. B. There are nodules of amniotic fluid debris attached to degenerated amnion.

B

Now includes full-color images

New Edition


AutopsyAiding the Living by Understanding Death (brochure, 50 copies)CAP Autopsy Committee

This popular “how-to” manual depicts both general and specialized techniques for performing autopsies in postmortem examinations in a hospital setting. Perfect for both pathologists in training and those who may not perform autopsies on a regular basis, the two-volume set includes an illustrated narrative description of autopsy methods as well as a set of step-by-step diagrams for use in the autopsy suite.

Designed to be used in conjunction with the comprehensive CAP publication, Autopsy Performance & Reporting, the contents include the complete autopsy, dissecting the organ block, pre-and post-dissection, perinatal and pediatric autopsies, the placenta, limited autopsies, embalmed bodies, and special techniques.

An Introduction to Autopsy Technique, 2nd editionKim A. Collins, MD, FCAP; Grover M. Hutchins, MD, FCAP

Special Autopsy Dissections is a unique resource that picks up where An Introduction to Autopsy Technique: Step-by-Step Diagrams leaves off. Presented in a similar format, the book contains clear, concise directions and detailed, step-by-step diagrams for numerous procedures, including information on the indications for the procedure and the equipment required. This book will serve as a ready aid for teaching, referral, and autopsy improvement.

Contents include the cardiac conduction system, air embolisms, esophageal varices, deep leg vein dissection, back and extremities cut-downs, disarticulations, en bloc pelvic dissection, peripheral nerve and skeletal muscle sampling, facial and neck dissection, vertebral artery and spinal cord dissection, removal of the temporal bone, and fetal brain removal. Appendices cover ancillary studies; CSF procurement; medical devices; decalcification; and fingerprinting of mummified, decomposed, and immersed hands.

Special Autopsy DissectionsKim A. Collins, MD, FCAP, editor

Written for the general public, this informative brochure provides individuals with an understanding of the autopsy and its role in advancing medical knowledge. It illustrates how families and communities benefit from autopsies, and addresses common concerns such as charges, funeral arrangements, organ retention, and religious conflicts. It also helps answer key questions such as:

• What is an autopsy?

• Why perform an autopsy?

• Who requests an autopsy?

Anatomic Pathology

Two-volume set: softcover plus laminated spiral-

bound illustrations; 162 pages; 2005

ISBN: 0-93030497-3

Item number: PUB119List Price: $75


Laminated, spiral bound; 50 pages, 2010ISBN: 978-093030497-3


SpecialAutopsyDissectionsStep-by-StepDiagrams

Kim A. Collins

AutopsyDissections:Layout 1 3/1/10 1:03 PM Page i


Member price: $32100 copies—$60/$48

Additional 50s—$15/$12


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Histologic PreparationsCommon Problems and Their Solutions Richard W. Brown, MD, FCAP, editor

Histologic Preparations: Common Problems and Their Solutions, developed by the CAP Histotechnology Committee in conjunction with the National Society for Histotechnology (NSH), is a how-to guide to good slide preparation. Building on data and images from the NSH/CAP HistoQIP program, the book presents photographic examples of well-prepared slides followed by numerous examples of associated problems and their solutions.The text contains troubleshooting techniques for the most common artifacts and problems incurred in routine histologic preparations, including fixation and processing; microtomy; frozen sections; hematoxylin-eosin, tricchrome, reticulin, elastin, basement membrane, mucin, amyloid, immunohistochemical, and Gram stains; and mycobacteria, Helicobacter pylori, sprirochetes, and fungi.

CAP Practical Guide to Gynecologic CytopathologyMorphology, Management, and Molecular MethodsDavid C. Wilbur, MD, FCAP; Michael R. Henry, MD, FCAP, editors

CAP Practical Guide to Gynecologic Cytopathology is an image-intense guide geared toward practitioners. This reference extensively covers the principals of gynecologic cytopathology, including evaluations of patients and their specimens, criteria to determine specimen adequacy, and comprehensive reviews of the morphology of the vast majority of entities, both benign and malignant, routinely identified in Pap tests. It is unique in that the morphology-based chapters heavily cover look-alikes and spectrums of cellular change, providing a more realistic framework for diagnosis than would the use of only classic illustrations.

Approximately 30,000 solid organ transplants are performed in the US each year, and biopsies from these patients are seen in community practice as well as in academic medical centers. Atlas of Transplant Pathology serves as a handy resource for practical interpretation of solid organ transplant biopsies and other specimens by general pathologists as well as subspecialists.

The book includes numerous high-quality full-color images and concise text, providing a framework for interpreting histologic patterns. Within the text, which contains the most recent ISHLT Working Formulation and Banff classifications, each organ system has its own section; and each diagnostic category under that section has its own chapter for ease of finding the information needed to assist in diagnosis. The succinct and highly illustrated format also serves as a convenient review for pathology residents, fellows, and others who must study for boards or other tests that include solid organ transplant pathology.

Atlas of Transplant Pathology Heart, Kidney, Liver, Lung, PancreasPhilip T. Cagle, MD, FCAP; Lisa Yerian, MD, FCAP; Luan D. Truong, MD, FCAP

Anatomic Pathology

Softcover; 600+ photomicrographs and tables; 2015ISBN: 978-098370686-1

Softcover; 270 pages; 700+ photomicrographs, figures, and tables; 2008ISBN: 978- 093030494-2

Item number: PUB121 List price: $95 Member price: $76Ebook price: $71

Softcover; 168 pages; 300+

photomicrographs, figures, and

tables; 2009 ISBN: 978-

093030495-9

Item number: PUB123


Ebook price: $37



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Handbook of Forensic Pathology is the perfect guide for forensic pathologists, medical examiners, and others who participate in medicolegal death investigation and forensic autopsies. Covering a broad range of topics, the text emphasizes forensic investigation and autopsy procedures, chain of custody, determination of time and manner of death, special procedures for examining and identifying the body, and specific circumstances surrounding natural and unnatural deaths.

Contents include postmortem changes, sudden unexpected deaths in adults and children, blunt and sharp force injury, craioncerebral trauma, shotgun wounds, asphyxia, deaths in custody, elder abuse, bodies found in water, sex- and sports-related deaths, multiple fatality incidents and terrorism, drug deaths, and deaths due to environmental hazards.

Handbook of Forensic Pathology, 2nd editionRichard C. Froede, MD, FCAP, editor

Basic Competencies in Forensic PathologyA Forensic Pathology PrimerCAP Forensic Pathology Committee

Basic Competencies in Forensic Pathology outlines the concepts and techniques used in forensic pathology. Prepared by the CAP Forensic Pathology Committee in conjunction with the National Association of Medical Examiners (NAME), the primer is intended to be a quick reference for pathologists in training and those who do occasional forensic autopsies. Based on the recommended guidelines endorsed by NAME for forensic pathology training in AP residency programs, the text aims to provide a minimum working knowledge of forensic pathology, address the differences between a hospital autopsy and a forensic (medicolegal) autopsy, explain the “what’s and why’s” of forensic autopsies, and help pathologists determine when a particular case might require more forensic expertise than they are able to provide.

Anatomic Pathology


ISBN: 0-93030489-6

Item number: PUB120

List price: $45Member price: $36

Ebook price: $33

Cause of Death and the Death Certificate is intended for any person who must prepare, review, or interpret cause-of-death statements appearing on death certificates or other medical documents, such as autopsy reports. This book contains updated information and has been broadened in content to provide information not only useful to physicians, medical examiners, and coroners, but to the public as well.

Contents include a summary of basic principles, protocol for writing cause-of-death statements for natural causes in general, in-depth discussions of death certification principles, examples of specific types of deaths that pose dilemmas or involve special considerations, and the NAME’s Guide for Manner of Death Classification.

Cause of Death and the Death Certificate Important Information for Physicians, Coroners, Medical Examiners, and the PublicRandy Hanzlick, MD, FCAP

Softcover, spiral bound;

244 pages; 2006ISBN: 0-93030490-X

Item number: PUB215


Ebook price: $28

Softcover; 589 pages; 100+ figures and tables; 2003ISBN: 0-93030479-9

Item number: PUB115 List price: $95Member price: $76Ebook price: $68


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PUB100 Autopsy: Aiding the Living by Understanding Death $32 $40

PUB115 Handbook of Forensic Pathology, 2nd edition 76 95

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PUB223 An Algorithmic Approach to Hemostasis Testing, 2nd Edition NEW EDITION 148 185

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PUB225 So You’re Going to Collect a Blood Specimen: An Introduction to Phlebotomy, 15th Edition NEW EDITION 28 35

PUB311 The Best of CAP TODAY Q&A 24 30

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PUB313 Atlas of Paleopathology 28 35

PUB314 Developing and Organizing an Institutional Biospecimen Repository 72 90

PUB315 The Healing Art of Pathology NEW 40 50

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ABRG Arthropod Benchtop Reference Guide 89 89

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2017 College of American Pathologists Publications Committee

Written and Edited by Recognized Experts

Katherine A. Galagan, MD, FCAP, Chair

Marilyn M. Bui, MD, PhD, FCAP

Philip T. Cagle, MD, FCAP

Samuel McCash, MD, FCAP

Gene P. Siegal, MD, PhD, FCAP

Mitchell S. Wachtel, MD, FCAP

Shi Wei, MD, PhD, FCAP

Thomas L. Williams, MD, FCAP

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Caryn L. Tursky, CAP Press

CAP Press is the publishing division of the College of American Pathologists, the world’s largest organization of board-certified pathologists and the leading provider of laboratory accreditation and proficiency testing programs. CAP Press offers outstanding books on:

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