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Xenoantigenicity of Porcine Decellularized ValvesMRK Helder MD, R Hennessy MD, R Dyer PhD, T Witt CVT, DB Spoon MD, RD Simari MD, JM Stulak MD, A Lerman MD

American Association for Thoracic SurgeryApril 28, 2105

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Disclsoure

• Funding – Dontation from H.H. Sheikh Hamed Bin Zayed Sultan Al-Nahyan

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BackgroundXenoantigenicity of Porcine Decellularized Valves

• Bioprosthetic valves fail• immune response?

• Decellularization should blunt

• α gal vs non α gal xenoantigens

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MethodsXenoantigenicity of Porcine Decellularized Valves

• Porcine aortic valves decell with 1% SDS

• Implanted into 3 sheepopulmonary position

• Serum obtained oPreopo1 wko1 moo2 mo

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A B C D

E F G H

Preop 1 week 1 month 2 months

Por

cine

Ovi

neResultsAntibody Reaction

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0

20

40

60

80

100

Time Point

An

ti-S

hee

p I

gM

Co

nce

ntr

atio

n u

g/m

L

*

0

20

40

60

80

100

Time Point

An

ti-S

hee

p I

gG

Co

nce

ntr

atio

n u

g/m

L

*

ResultsQuantitative Antibody Reaction

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Preop

Postop

0

2

4

6

Time Point

C1q

Co

nce

ntr

atio

n IU

/ml *

Preop

1 wee

k

1 m

onth

2 m

onth0

2

4

6

8

Time Point

C1q

Co

nce

ntr

atio

n IU

/ml

*

**

ResultsComplement

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A B C

C D

+ Control Sheep C

D8

Isot

ype

Con

trol

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SummaryXenoantigenicity of Porcine Decellularized Valves

• Evidence of IgG and IgM reaction

• Increased complement concentrations

• No T-lymphocyte role

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Conclusions

• Antibody and complement reaction to xeno valves

• Clinical implications are unclear and require further study

• SDS decellularization does not remove non-alpha gal antigens

• New products must be evaluated for antigenicity to ensure durability

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©2011 MFMER |

3149555-11

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