ISOLATION & IDENTIFIATION OF PETROLEUM ENGINE OIL DEGRADING BACTERIA FROM GARAGE SOIL AND EVALUATE THEIR DEGRADATION CAPABILITY

Under the Guidance of Presented by

Miss. Krati Jha Miss Neha chaurasia

Asst.prof. Of microbiology M.sc IV semester (Biotechnology)

Introduction

Diesel engine oil is the major product of crude oil.

It is a major source of pollution in our environment.

Petroleum is a aromatic compound. Oil is the Xenobiotic and recalcitrant. Diesel oil spills on agricultural land generally

reduced plant growth. Petroleum hydrocarbon can be degraded by

microorganism .

A large number of organism capable of degrading polyaromatic hydrocarbons compound.

Many different species , can degrade petroleum compound such as pseudomonas, micrococcus, bacillus, corneybactetium.

Hydrocarbon degrading bacteria are widely distributed in marine fresh water and soil habitats.

Oil degrading organism basically used alkane as a energy source and help in bioremediation.

Aims and objective

The Aim of our present study to isolate the

petroleum engine oil degrading bacteria from garage soil.

The Aim present study is to identify and evaluate their

degradation capability.

SAMPLE COLLECTION

Sample collection from garage.

ISOLATION METHOD

Spread plate method Streak plate method

Methodology

IDENTIFICATION

Biochemical testingIMVIC test Starch test Urease test

RESULT Gram staining negative Shape rod shaped

Colony orange

Urease negative

Gelatinase positive

H2s production negative

Indole production negative

Methyl red test negative

Voges proskauer test positive

Citrate test positive

Lactose negative

Sucrose positive

Glucose negative

Starch negative

Casien hydrolysis positive

Manni tol broth negative

Identified Acinetobacter may be

Colony of organism is found on the Enrichment media

Morphology of isolated strain after gram’s staining

Citrate agar test Urease agar test

Casein hydrolysis test

Gelatin agar test

conclusion

Bacteria are able to grow on to the enriched media and used diesel engine oil as a energy source.

The bacteria have high potential to degrading oil and producing biosurfactant layer due to which reduce a surface tension of soil and water.

Isolated bacteria is Acinetobacter sp. may be. Biochemical test were performed with

susceptibility was done with bacterial isolates. It can be used to in big level of future purpose

using a gene &produce level for future.

FURTHER ASPECTS

Soil sample can stored in -80c for further analysis.

carry out PCR and sequencing. identify 16s rna from potential bacteria.

THANK YOU