1 review how do molecular biologists identify genes in sequences of dna
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1 Review How do molecular biologists identify genes in sequences of DNA Use Analogies How is shotgun sequencing similar to doing a jigsaw puzzle - PowerPoint PPT PresentationTRANSCRIPT
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1 Review How do molecular biologists identify genes in sequences of DNA
Use Analogies How is shotgun sequencing similar to doing a jigsaw puzzle
2 Scientist one day may be able to use genomics to alter a child’s inherited traits. Under what circumstances, if any, should this be used and when should it not be used? Write a persuasive paragraph expressing your opinion by using specific examples of traits.
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CH 14 HUMAN HEREDITY14.3 Studying the Human Genome
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Cutting DNA
Restriction enzymes Cut DNA molecules into pieces, called restriction
fragments, at precise locations EcoRI restriction enzyme
Recognizes the sequence GAATTC Cuts each strand between the G and A
“Sticky Ends” The single strand overlap.
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Separating DNA
Gel electrophoresis Separate and analyze the differently sized DNA
fragments.
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Gel Electrophoresis
Mixture of DNA fragments is placed at one end of a porous gel
Electric voltage is applied and DNA molecules move toward the positive end
Smaller DNA fragment move farther and faster.
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Resulting pattern based on fragment size Add stains to see DNA Can remove individual fragments for further study.
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Reading DNA
Single-stranded DNA is placed in a test tube containing DNA polymerase and the four nucleotide bases
DNA polymerase uses the unknown strand as a template
Add a small number of bases that have a chemical dye attached one for each base Dye makes synthesis stop.
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Result is a series of color-coded DNA fragments of different lengths
Run gel electrophoresis Order of colored bands on the gel tells the exact
sequence of bases.
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The Human Genome Project
Main goal of sequencing all 3 billion base pairs of human DNA and identifying all human genes.
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Sequencing and Identifying Genes
Researchers mark the DNA strands . Find the base sequence in
different locations “Shotgun sequencing”
Cut DNA into random fragments, then determining the base sequence in each.
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Computer programs take the sequencing data and overlaps fragments
Align fragments relative to the known markers.
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Research Explores the Data from the Human Genome Project
Look for a promoter Shortly after is the reading
frame (become mRNA) Find start and stop codons,
and introns and exons.
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Comparing Sequences
People have very similar DNA 1 base in 1200 are different
SNPs (snips) Place where there is a single base difference
Haplotypes Closely linked SNPs that occur together May help identify diseases and conditions.
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Sharing Data
Bioinformatics Combines molecular biology with information science
Genomics Study of whole genomes, including genes and their functions.
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What We Have Learned
Working copy of the human genome June 2000 Full reference sequence April 2003 3 billion nucleotide bases Only about 2 percent of our genome encodes
instructions.
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What We Have Learned
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What We Have Learned
Pinpointed genes and associated particular sequences in those genes with numerous diseases and disorders
Identified about three million locations where single-base DNA differences occur in humans
Transferred important new technologies to the private sector, including agriculture and medicine.
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New Questions
Who owns and controls genetic information? Is genetic privacy different from medical privacy? Who should have access to personal genetic
information, and how will it be used?
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President George W. Bush signed into law the Genetic Information Nondiscrimination Act, which prohibits U.S. insurance companies and employers from discriminating on the basis of information derived from genetic tests.
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Modeling Restriction Enzymes
1. Write a random 50 base double strand DNA sequence using the 4 DNA bases. Include each sequence shown at least once
2. Make three copies of you double strand sequence on three different colored strips of paper
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3. Use the drawings to see how the restriction enzyme EcoRI would cut your sequence. Cut apart one copy of your sequence
4. Repeat step 3 using the restriction enzyme BamI on the second copy and the restriction enzyme HaeIII on the third
5. Tape the single strand end of one of your fragments to a complementary single strand end of your partner
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1. Observe Which restriction enzyme produced the most pieces and which the fewest
2. Evaluate How well did your model represent the actual process of using restriction enzymes to cut DNA. Contrast the length of your model to the actual length of a DNA molecule