Injection Date : 17 Apr 18 6:27 pm +0800 Seq. Line : 70Location : P2-A-01

Acq. Operator : SYSTEM Inj : 1 Spec. Reported : MS Integration Inj Volume : 1 ul Acq. Method : D:\LCMS03\Data\2018\20180417\20180417-1\1-POS-3MIN.M Analysis Method : D:\LCMS03\Data\2018\20180417\20180417-1\1-POS-3MIN.M

Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA)Gradient: 5% to 95%B within 1.3 minFlow Rate :1.8ml/minColumn :SunFire C18, 4.6*50mm,3.5um A-RP-186Oven Temperature : 45C

min0 0.5 1 1.5 2 2.5

mAU

0

250

500

750

DAD1 A, Sig=214,4 Ref=off

1.832

1.907

1.990

2.031

min0 0.5 1 1.5 2 2.5

mV

25

50

75

100

ELS1 A, ELSD Signal1.303

1.979

min0 0.5 1 1.5 2 2.50

500000

1000000

1500000

MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 701.937

min0 0.5 1 1.5 2 2.50

200

400

600

Ion 362.8, MSD1 362.8,Target Mass 361.8 +H Positive, EIC=362.5:363.5

Integration Results for DAD1 A, Sig=214,4 Ref=off

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.83 0.02 3.79 3.52 0.32 102 1.91 0.02 1188.81 1130.13 99.31 745 1.99 0.03 3.90 2.57 0.33 745 2.03 0.02 0.56 0.45 0.05 105

Integration Results for ELS1 A, ELSD Signal

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.30 0.08 14.92 2.75 4.15 102 1.98 0.05 344.38 105.97 95.85 745

Integration Results for MSD1 TIC, MS File

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.94 0.06 6708976.50 1772711.63 100.00 745

Page 1 of

LCMS Report

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Sample Name : TAK-242-04

2

File ..018\20180417\20180417-1\TAK-242-04.D Tgt Mass (CHM):

Ret. Time: 1.94 <<<< POSITIVE SPECTRA >>>>

m/z100 200 300 400 500 600 700 800 900

0

10000

20000

30000

40000

50000

60000

70000

ES-API Positive

385.1

750.1

233.1

425.1

299.1

105.1

153.1 384.1

746.1

298.1

747.1745.1

Page 2 of

LCMS Report

2