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1-2 How Scientists Work Slide 1 of 32 Copyright Pearson Prentice Hall Designing an Experiment Forming a Hypothesis One early hypothesis was spontaneous generation, or the idea that life could come from nonliving matter. For example, most people thought that maggots spontaneously appeared on meat. In 1668, Redi proposed a different hypothesis: that maggots came from eggs that flies laid on meat.

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Page 1: 1-2 How Scientists Work Slide 1 of 32 Copyright Pearson Prentice Hall Designing an Experiment Forming a Hypothesis One early hypothesis was spontaneous

1-2 How Scientists Work

Slide 1 of 32

Copyright Pearson Prentice Hall

Designing an Experiment

Forming a Hypothesis

One early hypothesis was spontaneous generation, or the idea that life could come from nonliving matter. For example, most people thought that maggots spontaneously appeared on meat.

In 1668, Redi proposed a different hypothesis: that maggots came from eggs that flies laid on meat.

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Designing an Experiment

Redi’s Experiment

Controlled Variables:jars, type of meat,location, temperature,time

Covered jarsUncovered jars

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Designing an Experiment

Redi’s Experiment

Manipulated Variable:Gauze covering that keeps flies away from meat

Responding Variable:whether maggots appear Maggots appear.

Severaldays pass.

No maggots appear.

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Designing an Experiment

Redi’s Experiment

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Designing an Experiment

Drawing a Conclusion

Scientists use the data from an experiment to evaluate a hypothesis and draw a valid conclusion.

Redi’s results supported the hypothesis that maggots were produced by flies, not spontaneous generation.

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Repeating Investigations

Needham's Test of Redi's Findings

Needham challenged Redi’s results by claiming that spontaneous generation could occur under the right conditions.

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Repeating Investigations

Needham’s Test of Redi’s Findings

• Needham sealed a bottle of gravy and heated it.

• After several days, the gravy was swarming with microorganisms.

• Needham concluded that these organisms came from the gravy by spontaneous generation.

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Repeating Investigations

Spallanzani's Test of Redi's Findings 

Gravy is boiled. Gravy is boiled.

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Repeating Investigations

Spallanzani's Test of Redi's Findings 

Flask is open. Flask is sealed.

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Repeating Investigations

Spallanzani's Test of Redi's Findings 

Gravy is teemingwith microorganisms.

Gravy is free ofmicroorganisms.

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Repeating Investigations

Pasteur's Test of Spontaneous Generation

• Louis Pasteur conclusively disproved the hypothesis of spontaneous generation.

• Pasteur showed that all living things come from other living things.

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Repeating Investigations

Pasteur’s Experiment

Broth is boiledBroth is freeof microorganismsfor a year.

Curved neck isremoved.

Broth is teeming with microorganisms.

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Repeating Investigations

The Impact of Pasteur’s Work

Pasteur saved the French wine industry, which was troubled by unexplained souring of wine.

He saved the silk industry, which was endangered by a silkworm disease.

He began to uncover the nature of infectious diseases, showing that they were the result of microorganisms.

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How a Theory Develops

How a Theory Develops

As evidence from numerous investigations builds up, a hypothesis may become so well supported that scientists consider it a theory.

In science, the word theory applies to a well-tested explanation that unifies a broad range of observations.

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How a Theory Develops

No theory is considered absolute truth.

As new evidence is uncovered, a theory may be revised or replaced by a more useful explanation.