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Design and Manufacture of Plants for the Production of:

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Bioreactors and FermentersAirlift FermenterAirlift Visual Safety

Fermenter VSFAnaerobic FermenterAutoclavable Fluidized Bed

Reactor AWSAutoclavable Laboratory

Fermenter ALFCell FermenterEnzyme Membrane Reactor

EMRFermenter with Integrated

DownstreamingFermenter for Plant Cell

Cultivation Fluidized Bed Reactor WSHigh Pressure FermenterHigh-Temperature-High-

Pressure Fermenter HTELaboratory Fermenter L1523Laboratory Fermenter NLF22Laboratory Pilot

Fermenter LP351Loop Safety Fermenter LSFMembrane Reactor MFNMR ReactorPhotoreactorPilot Plant FermenterRotaschon FermenterSmall Laboratory

Fermenter KLFSolid State FermenterSurface Cultivation Reactor Visual Safety Fermenter VSF

Up- and Downstream EquipmentCold SterilizationContinuous Sterilization

SystemDispersing MixerDosage SystemMedium Storage Vessel

50–5000 lPerfusion SystemSpiral FilterFeed Vessel

Probes/Measuring systemsAdmittance ProbeAntifoam ProbeExhaust Gas AnalyzerGas Flow MeterIFM - Intelligent Front

ModuleLevel Indicator «Biowatch»Level Measurement ProbepH/Redox ProbepH Interchangeable ProbepO2 probe pO2 Interchangeable ProbePressure TransmitterPt100 Temperature Probe Software for Media

OptimizationTurbditiy ProbeWeighing ProbeWeight Measurement:

Platform, Cell

Sampling and HarvestingContained Sampling SystemDialysis ProbeHarvesting ValvePervaporation ProbeSampling Valve

Agitation SystemDirect DriveDisk TurbineDraught TubeMagnetic DriveTop DriveImpeller

Aeration and ExhaustAutosterile FilterAir FilterBubble-Free AerationControl Valve Flow MeterGas Mixing StationIncineratorReflux CoolerViewing Glass Bursting

Disk Assembly

Vessel AccessoriesBottle Cap with Sterile Filter Bubble Trap CIP Spray BallCIP ValveDosing Valve Foam BreakerHypodermic Needle with

Sterile Case AssemblyIllumination UnitInjector ValveLamella Clarifier Unit Lid Opening DeviceRotor FilterRupture disk Safety ValveSteam TrapSterile Pressure GaugeSterile TrapThermometer Viewing Port

Pumps for Sterile ApplicationsDomp® Double Diaphragm

PumpDouble Piston PumpKobio-Pump Magnetic Circulation PumpPeristaltic PumpPiston Diaphragm Metering

Pump

Components for Sterile LinesDiaphragm ValveMultiple-Diaphragm ValveMemtile: Stainless-Steel

Valve DrivePneumatic Valve DrivePipe-Pt100Sterile ConnectionTRI-Clamp ConnectionVacuum Valve

Fermenter EnergiesCIP-System: Mobil, KitchenClean Steam GeneratorCompressorCoolerHeating CircuitSteam GeneratorThermostat

Powder Handling/MixingCup Hermann Powder

Transfer SystemInversina Tumbler Mixer,

2–500 LitresManual mini-Inversina

tumbler mixer

Amino AcidsAntibioticsBiomass: Probiotics,

Starter Cultures

Measurement and controlBioLogicsBack up

BiopesticidesBiopolymers and LipidsEnzymesEthanol

Data loggingData processingProcess analysis

Process managementProcess optimization Process supervision

Flavors and FragrancesFruit JuicesMonoclonal AntibodiesOrganic Acids

Peptides/Proteins: BloodFactors, Hormones,Growth Factors

Sugar Derivates

VaccinesVitamins

Bioengineering AG Sagenrainstrasse 7 CH-8636 Wald, Switzerland Telephone ++41 55 256 8 111 Fax ++41 55 256 8 256info@bioengineering.ch www.bioengineering.ch

Bioengineering – Experience onlyspecialists can have

Laboratory Pilot Fermenter LP351The link between laboratory and production

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Standard Optional1 Vessel stainless steel 316 L special alloys

grain 240 mirrorpolished

2 Heating circuit closed circuit, galvanized steel, stainless steel 316 L, electrical heat exchanger for steam heater, heat exchanger for cooling

circuits

3 Agitation bottom drive by belt, top drive, magnetic couplingdouble mechanical seal

4 Mechanical seal silicium carbide, steam condensate lubricatedglycerine lubricated

5 Stirrer blade stirrers, baffles airlift, draught tube

6 Inlet air Autosterile filter, ceramic filter separate filter, double filters, absolute filter, gas mixing station

7 Aeration submerse aeration surface aeration, bubble-free aeration

8 Ports standardized lid and side ports flexible location and number special ports

9 Viewing glasses longitudinal and lid viewing flexible location and numberglass with illumination

10 Exhaust air ceramic filter absolute filter, reflux cooler, foam destruction, incinerator

11 Inoculation inoculation port fixed piping, transfer concepts

12 Sampling manual sampling valve pneumatic valve, contained sampling valve, valve with steel bellow, cell-free sampling system

13 Harvest manual harvest valve with steel pneumatic harvest valve, fixedbellows piping, transfer concept

14 Media addition needle connection through lid dosage valve, coupling, fixed piping, transfer concepts

Sterilization manual sterilization of Autosterile automatic sterilization of Autosterilefilter and exhaust air valve filter and exhaust air valve

Double mechanical seal

Magnetic stirrers

The fermenter unit isinstalled on a platform.Easy maintenance andservice work on thefermenter parts arepossible with the openframe construction.

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Product overviewtotal volume working vol. width x depth DN H1 H2 H342 l 28 l 800 x 800 260 1410 860 80050 l 33 l 800 x 800 295 1410 860 80075 l 50 l 800 x 850 345 1430 870 815

Technical dataoperating temperature max. 150° Cmaterial of parts which are in contact with the product 316 Lmotor power 42 l, 50 l 2,2 kW

75l 3 kWspeed max. 1000 min–1

Scale-up or production processes are the field of application for theLaboratory Pilot Fermenter LP351 with total volume 42, 50 or 75 litres.

The straightforward concept in designand construction results in a user-friendly and sturdy high-tech plant.The central in- and outlets for steam,water, air, and waste water, minimizeinstallation time and work.

The flexible installation of Bioengi-neering probes and accessories formedia addition, aeration, sampling,harvest, perfusion, foam destruction,CIP, etc. is guaranteed with thestandardized ports.

The modular design in combinationwith the variety of proven accessoriesenable the performance of biotech-nological processes with enzymes,microorganisms, fungi, algae, plant or animal cells.

Our equipment fulfils the requirednational guidelines and cGMP/FDA-regulations (including IQ and OQ documentation on request).

The stirrer units is driven by belt, thusreducing the fermenter height, sepa-rating the vessel from the motor, andoptimizing the bearing of the free-swinging shaft. The shaft is removedfrom the top and the bearing with the seals is easily disassembled fromthe bottom.

The fermenter is aerated through the Bioengineering Autosterile filter.Without any complicated peripheralconstructions, this filter is in-situsterilizable together with the fer-menter.

The power supply and the measure-ment and control units in the 19”measurement cabinet are separatedfrom the fermenter. The Bioengi-neering IFM controllers combine stateof the art digital technology with a modular set-up. Each measurementor control loop has its own separatemodule, thus enabling easy mainte-nance of single units or the adaptionof the fermenter measurement andcontrols according to actual require-ments. The BioLogics- or BioVisual-software covers all data logging andvisualization requirements. On requestother software systems are available.