1. Antibodies and vaccines

2. Traditional polyclonal antibody preparations: passive - Antisera immunoglobulin preparations. IgG 3. Antisera immunoglobulin - - Antisera - - - 4. NaCl 0.9% -- 0.25% 5. Monoclonal antibodies 6. Antibody screening: phage display technology E.Coli 7. Unique surface antigene 8. Tumour immunology NK cells 9. CEACarcinoembryonic antigen ricin, pokeweed toxin, Pseudomonas toxin 10. -fetoprotein (AFP) CEA - CA125 11. Humanization - 12. Antigen Binding Site Variable Regions Constant Regions Effector Functions 13. Chimeric mAb 14. Chimeric mAb 15. CHIMERIC HUMAN/MOUSE FR1 FR2 FR3 FR4 CDR1 CDR2 CDR3 CONSTANT CONSTANT CONSTANT FR1 FR2 FR3 FR4 CDR1 CDR2 CDR3 Mouse Human Chimeric 16. Human Mouse Chimeric 17. HUMANIZED ANTIBODIES FR1 FR2 FR3 FR4 CDR1 CDR2 CDR3 CONSTANT CONSTANT CONSTANT FR1 FR2 FR3 FR4 CDR1 CDR2 CDR3 Mouse Human Humanized 18. Human Mouse Humanized 19. Vaccination T B -- - - 20. Passive - - - - 21. Active Attenuated Killed Purified components or macromolecules 22. VaccinesAttenuated Bacillus Calmette-Guerin Sabin poliovirus 23. - 24. 25. Killed Vaccines - - 26. 27. 28. Bacterial Polysaccharide Capsules Toxoids Proteins from Pathogens Produced by Recombinant Techniques Synthetic Peptides Naked Gene Anti-idiotype vaccines 29. Bacterial Polysaccharide Capsules T TT conjugate vaccine 30. Toxoids 31. Synthetic Peptides 32. Naked Gene CpG gene gun 33. - - - 34. Adjuvants - -A LPS AMLA -T INF-yTh1 -B