wt tg
DESCRIPTION
IL-2. CONTROL. CD3/CD28+IL-2. CD3/CD28. 74.3 9.9. 89.4 6.8. 45.4. 52. 50.7. 47.9. WT TG. CD2. 41.5. 56. 85.4. 10.6. 56.1. 42.2. 9.1. 84.1. CD25. FIGURE 1. WT. TG. 20000. 18000. 16000. 14000. Thymidine Uptake (cpm). - PowerPoint PPT PresentationTRANSCRIPT
WT
TG
74.3 9.9 5245.4
CD
2
CD3/CD28
84.1 9.1 42.2 56.1 41.5 56
CD25
47.9
CONTROL CD3/CD28+IL-2
50.789.4 6.8
85.4 10.6
IL-2
FIGURE 1
0
2000
4000
6000
8000
10000
12000
14000
16000
18000
20000
Co
ntr
ol
C
D3
/C
D2
8
IL-2
IL-2
+
CD
3/
CD
28
WT TG
Co
ntr
ol
C
D3
/C
D2
8
IL-2
IL-2
+
CD
3/
CD
28
Th
ym
idin
e U
pta
ke
(c
pm
)
FIGURE 2
0
0,5
1
1,5
2
2,5
0200
400
600
800
1000
1200
0
20
40
60
80
100
120
010203040506070
0
0,1
0,2
0,3
0,4
0,5
0,6 **
** **
IL-1
0 (n
g/m
L)
IL-4
(p
g/m
L)
IL-1
3 (p
g/m
L)
IFN
- (
ng
/mL
)IL
-2 (
ng
/mL
)
Unstimulated
Anti-CD3/CD28
FIGURE 3
WT TG WT TG
IL-2 + anti-CD3/28IL-2
**
0
10
20
30
40
50
60
anti-CD3/28Unstimulated
CD4+
CD8+
* *
0
10
20
30
40
50
60
70
70*
FIGURE 4
PE
RC
EN
TA
GE
OF
CE
LL
NU
MB
ER WT
WT
WT
WT
TG TG
TG TG
*
CD4+
CD8+
PE
RC
EN
TA
GE
OF
CE
LL
NU
MB
ER
FIGURE 5
0
10
20
30
40
50
60
70
T CD4 CD8
% C
EL
L N
UM
BE
R
*
0
20
40
60
80
100
120
140
CE
LL
NU
MB
ER
WTTG
WT TG
*