wp3
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CNR IAMC - Istituto per l’Ambiente Marino Costiero Messina, Italy Università degli Studi di Messina. WP3. State of the Art April 2003. Active DHABs bacteria WP3 Preliminary data. Phylogenetical Analysis. Desulfobulbus elongatus – 22%. Uncultured Clone Above Hydrate Ridge_SRB- 22%. 14%. - PowerPoint PPT PresentationTRANSCRIPT
WP3WP3WP3WP3
State of the Art April 2003State of the Art April 2003
CNRCNRIAMC - Istituto per l’Ambiente Marino IAMC - Istituto per l’Ambiente Marino
CostieroCostieroMessina, ItalyMessina, Italy
Università degli Studi di MessinaUniversità degli Studi di Messina
CNRCNRIAMC - Istituto per l’Ambiente Marino IAMC - Istituto per l’Ambiente Marino
CostieroCostieroMessina, ItalyMessina, Italy
Università degli Studi di MessinaUniversità degli Studi di Messina
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14% Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN Drine-Interface - 22% Uncultured Clone from SHABAN Drine-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 22%
Chryseobacterium_meningosepticum - 22% Flexibacter_flexilis -14%
Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteria WP3Active DHABs bacteria WP3Preliminary dataPreliminary data
22%
14%
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14%
Salt Marsh Clone – 14% - 22%Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN BRINE-Interface - 22%Uncultured Clone from SHABAN BRINE-Interface - 22% Uncultured Clone from SHABAN BRINE-Interface - 22%Uncultured Clone from SHABAN BRINE-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 32.5% Chryseobacterium_meningosepticum - 22%
Flexibacter_flexilis -14% Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14%Uncultured KB1 – 14%
Uncultured KB1 – 22% - 22%Uncultured KB1 – 22% - 22% OP11- 22%OP11- 22%
Sulfolobus_acidocaldarius Methanobrevibacter_sp-14% - 14%
Uncultured crenoarcheota-22 Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14%Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14%
Archeoglobus_fulgidus - 22% Methanosarcina_acetivorans - 22% - 21%
Uncultured Archeon GBa1r010 - 22%0.05
Deep (600-1000mt)Anoxic
Hypersaline Environments
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteriaActive DHABs bacteriaPreliminary dataPreliminary data
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14% Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN BRINE-Interface - 22% Uncultured Clone from SHABAN BRINE-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 32.5% Chryseobacterium_meningosepticum - 22%
Flexibacter_flexilis -14% Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
Methane enrichedenvironment
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteriaActive DHABs bacteriaPreliminary dataPreliminary data
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14% Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22%
Alkanivorax borkumensis - 22%Alkanivorax borkumensis - 22% Pseudomonas putida - 22% -14% -14% -14%Pseudomonas putida - 22% -14% -14% -14%
Acinetobacter calcoacetics – 22% Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN BRINE-Interface - 22% Uncultured Clone from SHABAN BRINE-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 32.5% Chryseobacterium_meningosepticum - 22%
Flexibacter_flexilis -14% Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteriaActive DHABs bacteriaPreliminary dataPreliminary data
Phylogenetical AnalysisPhylogenetical Analysis
Our Isolates
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14% Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN BRINE-Interface - 22% Uncultured Clone from SHABAN BRINE-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 22%
Chryseobacterium_meningosepticum - 22% Flexibacter_flexilis -14%
Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteria WP3Active DHABs bacteria WP3Preliminary dataPreliminary data
22%
14%
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14% Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN BRINE-Interface - 22% Uncultured Clone from SHABAN BRINE-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 22%
Chryseobacterium_meningosepticum - 22% Flexibacter_flexilis -14%
Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteria WP3Active DHABs bacteria WP3Preliminary dataPreliminary data
22%
14%
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14% Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22% Bradyrhizobium_japonicum-14%
Uncultured SB1-22% Uncultured Clone from SHABAN Drine-Interface - 22% Uncultured Clone from SHABAN Drine-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 22%
Chryseobacterium_meningosepticum - 22% Flexibacter_flexilis -14%
Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteria WP3Active DHABs bacteria WP3Preliminary dataPreliminary data
22%
14%
Desulfobulbus elongatus – 22% Uncultured Clone Above Hydrate Ridge_SRB- 22%
Uncultured Sheban Brine-interface Water - 22% Syntrophobacter_pfennigii- 22% Desulfobacterium_anilini-14%
Unculured_Delta_Seep_Methan- 22% Unc_Hydrocarbon_Seep_Bac- 22%
Pelobacter_acetylenicus-22% MERTZ 2CM 309- 22%
wb1 H02- 22% Caldithrix_abissy-14%Caldithrix_abissy-14%
Salt Marsh Clone – 14% - 22% Pseudomonas hibiscicola - 22%
Xanthomonas melonis - 22% - 22% Alkanivorax borkumensis - 22%
Pseudomonas putida - 22% -14% -14% -14% Acinetobacter calcoacetics – 22%
Vibrio mediterranei-22% Janthinobacterium_lividum-22%
Uncultured_Duganella_clone_CTHB-18-22%
Bradyrhizobium_japonicum-14%Bradyrhizobium_japonicum-14% Uncultured SB1-22%
Uncultured Clone from SHABAN Drine-Interface - 22% Uncultured Clone from SHABAN Drine-Interface - 22%
Deinococcus_radiodurans_DSM_20539 - 22% Chryseobacterium_meningosepticum_ATCC_13253 – 14% -14% - 22%
Chryseobacterium_meningosepticum - 22% Flexibacter_flexilis -14%
Leptolyngbya_foveolarum- 22% - 14% - 14% Dehalococcus etenogenes - 22%
Green non Sulfur Bacteria - 22% - 22% Uncultured KB1 – 14% Uncultured KB1 – 22% - 22%
OP11- 22% Sulfolobus_acidocaldarius
Methanobrevibacter_sp-14% - 14% Uncultured crenoarcheota-22
Uncultured_euryarcheota MERTZ 2CM136 – 14% - 14% Archeoglobus_fulgidus - 22%
Methanosarcina_acetivorans - 22% - 21% Uncultured Archeon GBa1r010 - 22%0.05
Phylogenetical AnalysisPhylogenetical Analysis
URA clones URA clones analysisanalysis(Bannock)(Bannock)
Active DHABs bacteria WP3Active DHABs bacteria WP3Preliminary dataPreliminary data
22%
14%